Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,658,893 G→A *176* (TAG→TAA gadE → gad regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,658,8930GA100.0% 374.6 / NA 114*176* (TAG→TAAgadEgad regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (57/57);  total (57/57)

Only 100 of 114 total aligned reads displayed.

ACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGT  >  NC_000913/3658780‑3659022
                                                                                                                 |                                                                                                                                 
aCGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGa                                                                                                          >  2:915886/1‑139 (MQ=255)
  gCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCAGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtg                                                                                                        >  2:1428672/1‑139 (MQ=255)
   cTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       >  2:1482823/1‑139 (MQ=255)
   cTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       <  1:1428672/139‑1 (MQ=255)
     cAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggct                                                                                                     <  1:915886/139‑1 (MQ=255)
      aaTATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctg                                                                                                    <  1:156981/139‑1 (MQ=255)
             cGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       >  2:1495753/1‑129 (MQ=255)
             cGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       <  1:1495753/129‑1 (MQ=255)
                aacaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGTACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             >  2:177926/1‑136 (MQ=255)
                aacaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             <  1:177926/136‑1 (MQ=255)
                aacaaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAg                                                                                          <  1:1546111/139‑1 (MQ=255)
                   aaacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGa                                                                                       <  2:403628/139‑1 (MQ=255)
                   aaacaTTAAAGAATCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAATCAACTGAAATTGATGGCTGGCATGACGAGGGa                                                                                       <  2:1031641/139‑1 (MQ=255)
                                tCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAt                                                                          <  2:15891/139‑1 (MQ=255)
                                 cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATc                                                                                                                                 >  1:809661/1‑83 (MQ=255)
                                 cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                           <  1:84199/137‑1 (MQ=255)
                                 cAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                           >  2:84199/1‑137 (MQ=255)
                                                 tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       >  2:360461/1‑93 (MQ=255)
                                                 tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       <  1:360461/93‑1 (MQ=255)
                                                 tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGa                                                         >  2:1372155/1‑139 (MQ=255)
                                                  gTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCATGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                        <  2:1093114/139‑1 (MQ=255)
                                                    aCAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTa                                                      >  1:18813/1‑139 (MQ=255)
                                                      aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGa                                                                                                                <  2:1512096/79‑1 (MQ=255)
                                                      aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATg                                                                                     <  1:993166/106‑1 (MQ=255)
                                                      aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCCGATGCTGATTTCAGTACCCCCGGTGATTACt                                                    >  1:1065673/1‑139 (MQ=255)
                                                      aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAg                                                                                  <  2:1182841/109‑1 (MQ=255)
                                                      aaGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAg                                                                                  >  1:1182841/1‑109 (MQ=255)
                                                       aGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTa                                                   >  1:1416717/1‑139 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCa                                                                                                                     <  1:289454/72‑1 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCa                                                                                                                     >  2:289454/1‑72 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc                                                               >  2:422350/1‑126 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAcccc                                                               <  1:422350/126‑1 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa                                                  >  2:1399349/1‑139 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa                                                  >  2:1600960/1‑139 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa                                                  >  2:1683611/1‑139 (MQ=255)
                                                        gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTaa                                                  >  2:1154778/1‑139 (MQ=255)
                                                           gAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             >  2:627448/1‑93 (MQ=255)
                                                           gAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             <  1:627448/93‑1 (MQ=255)
                                                            aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTg                                                                            >  1:1169223/1‑109 (MQ=255)
                                                            aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTg                                                                            <  2:1169223/109‑1 (MQ=255)
                                                            aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga                                              <  1:1372155/139‑1 (MQ=255)
                                                               cTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGagag                                           <  1:1482823/139‑1 (MQ=255)
                                                                  aGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAgg                                                                                         >  2:271883/1‑90 (MQ=255)
                                                                  aGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAgg                                                                                         <  1:271883/90‑1 (MQ=255)
                                                                      gTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAg                                                                                          >  2:1153220/1‑85 (MQ=255)
                                                                      gTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAg                                                                                          <  1:1153220/85‑1 (MQ=255)
                                                                        aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGt                                                           <  1:561924/114‑1 (MQ=255)
                                                                        aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGt                                                           >  2:561924/1‑114 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             <  1:863192/79‑1 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             >  2:863192/1‑79 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTg                                                          >  2:956907/1‑114 (MQ=255)
                                                                         tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGa                                 >  2:211346/1‑139 (MQ=255)
                                                                           aGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACt                               >  1:1310852/1‑139 (MQ=255)
                                                                              gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                            <  1:1399349/139‑1 (MQ=255)
                                                                              gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                            <  1:211346/139‑1 (MQ=255)
                                                                              gACATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                            <  1:914003/139‑1 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                  <  1:587121/67‑1 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                  >  2:587121/1‑67 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGc                                                                                    >  1:1656411/1‑81 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGc                                                                                    <  2:1656411/81‑1 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCa                                                                                   >  2:1032475/1‑82 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCa                                                                                   <  1:1032475/82‑1 (MQ=255)
                                                                                cATCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACACCCGGTGATTAATAAAGGAGAGGCTAAAACGACTTTAtt                          >  2:1429626/1‑139 (MQ=255)
                                                                                 aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                  >  1:778259/1‑66 (MQ=255)
                                                                                 aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                  <  2:778259/66‑1 (MQ=255)
                                                                                 aTCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTc                         >  2:292155/1‑139 (MQ=255)
                                                                                  tCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                  <  2:1252095/65‑1 (MQ=255)
                                                                                  tCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                  >  1:1252095/1‑65 (MQ=255)
                                                                                  tCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                        <  1:1498465/107‑1 (MQ=255)
                                                                                  tCGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcc                        <  1:636622/139‑1 (MQ=255)
                                                                                   cGTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc                       <  1:1273602/139‑1 (MQ=255)
                                                                                    gTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc                      <  2:1093364/139‑1 (MQ=255)
                                                                                    gTCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc                      <  1:1600960/139‑1 (MQ=255)
                                                                                     tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtggctggc                                                                                                  >  1:1372531/1‑62 (MQ=255)
                                                                                     tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAccc                                                                >  1:1369963/1‑96 (MQ=255)
                                                                                     tCACCCTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTAccc                                                                <  2:1369963/96‑1 (MQ=255)
                                                                                          cTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt                                                                     >  2:1546030/1‑86 (MQ=255)
                                                                                          cTGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt                                                                     <  1:1546030/86‑1 (MQ=255)
                                                                                           tGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga                                              <  1:1370974/108‑1 (MQ=255)
                                                                                           tGGGTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGga                                              >  2:1370974/1‑108 (MQ=255)
                                                                                            gggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt                                                                     >  1:1032123/1‑84 (MQ=255)
                                                                                            gggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt                                                                     <  2:1032123/84‑1 (MQ=255)
                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             >  1:1212155/1‑59 (MQ=255)
                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             <  2:1212155/59‑1 (MQ=255)
                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt                                                                     <  2:383391/83‑1 (MQ=255)
                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                        <  2:673341/96‑1 (MQ=255)
                                                                                             ggTATCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAtgtg             >  2:455054/1‑139 (MQ=255)
                                                                                                aTCACATCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTAt          <  1:292155/139‑1 (MQ=255)
                                                                                                  cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       <  1:880249/44‑1 (MQ=255)
                                                                                                  cacaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGAtgg                                                                                                       >  2:880249/1‑44 (MQ=255)
                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                           <  2:32065/71‑1 (MQ=255)
                                                                                                   acaTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGa                                                                           >  1:32065/1‑71 (MQ=255)
                                                                                                    caTCTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATccac      <  1:1154778/139‑1 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGGCGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGAt                                                        >  2:137885/1‑86 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                            >  1:408352/1‑114 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTa                            <  2:408352/114‑1 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc                      <  1:1422942/120‑1 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTcccc                      >  2:1422942/1‑120 (MQ=255)
                                                                                                       cTTATTTTTAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg   >  1:1581715/1‑139 (MQ=255)
                                                                                                           tttttAATCAGGACATAAGCAACTGAAATTGATGGCTGGCATGAc                                                                                             <  1:399141/45‑1 (MQ=255)
                                                                                                                 |                                                                                                                                 
ACGCTCAATATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCATTACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGT  >  NC_000913/3658780‑3659022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: