| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,783,086 | G→A | G192G (GGC→GGT) | gpsA ← | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,783,086 | 0 | G | A | 91.4% | 186.2 / 8.8 | 70 | G192G (GGC→GGT) | gpsA | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (35/29); total (38/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-01 | |||||||||||
CAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGT > NC_000913/3782950‑3783212 | cAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGcacc > 2:536954/1‑139 (MQ=255) cccAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCa < 2:251961/139‑1 (MQ=255) cgccgcACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCa < 1:406329/139‑1 (MQ=255) cgcACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATg < 1:536954/139‑1 (MQ=255) aGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATGCCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCg > 2:1183117/1‑139 (MQ=255) aCGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGa < 1:1629248/139‑1 (MQ=255) tttCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTa < 2:805520/139‑1 (MQ=255) tCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTaaa > 1:1051399/1‑139 (MQ=255) cccACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaaa > 1:471945/1‑139 (MQ=255) cccACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaaa > 1:317440/1‑139 (MQ=255) aTCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTg > 1:1440006/1‑92 (MQ=255) aTCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTg < 2:1440006/92‑1 (MQ=255) aTCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACg > 1:118167/1‑96 (MQ=255) aTCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACg < 2:118167/96‑1 (MQ=255) cgcCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgc > 2:832455/1‑139 (MQ=255) ccGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcag > 2:597178/1‑139 (MQ=255) tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg < 2:389592/128‑1 (MQ=255) tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg > 1:389592/1‑128 (MQ=255) tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGcagcag > 1:510655/1‑139 (MQ=255) cATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAgctgc > 2:498535/1‑137 (MQ=255) cATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAgctgc < 1:498535/137‑1 (MQ=255) tCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGgag > 2:114222/1‑139 (MQ=255) tCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGgag < 1:114222/139‑1 (MQ=255) aCCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGatca > 2:1492495/1‑139 (MQ=255) aCCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCa < 2:1051399/139‑1 (MQ=255) tCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGAt < 2:317440/139‑1 (MQ=255) ggACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTg < 1:1117531/51‑1 (MQ=255) ggAAATCCCCGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTg > 2:1117531/1‑51 (MQ=38) ccccGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAg > 1:435356/1‑139 (MQ=255) ccccGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAg > 2:959036/1‑139 (MQ=255) cccGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAgg > 2:335168/1‑139 (MQ=255) ccGCACCAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGc < 1:832455/139‑1 (MQ=255) cACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAg < 1:871901/139‑1 (MQ=255) cAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg < 2:968273/83‑1 (MQ=255) cAATGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg > 1:968273/1‑83 (MQ=255) aTGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCgg < 1:183039/53‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCgg > 2:183039/1‑53 (MQ=255) aTGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGatca < 1:1486677/105‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGatca > 2:1486677/1‑105 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGc > 2:598883/1‑79 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGc < 1:598883/79‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg > 2:1029130/1‑81 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg < 1:1029130/81‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcagc > 1:341352/1‑90 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcagc < 2:341352/90‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGatcat > 2:1586732/1‑106 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGatcat < 1:1586732/106‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCa < 1:1522850/111‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCa > 2:1522850/1‑111 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGAt > 1:587662/1‑121 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGAt < 2:587662/121‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGAt > 2:81138/1‑121 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGAt < 1:81138/121‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCgg < 1:256343/124‑1 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCgg > 2:256343/1‑124 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGaaa > 1:1215298/1‑139 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGaaa > 2:1622072/1‑139 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGaaa > 1:1267623/1‑139 (MQ=255) aTGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGaaa > 1:828346/1‑139 (MQ=255) tGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGa < 1:1082324/53‑1 (MQ=255) tGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGa > 2:1082324/1‑53 (MQ=255) tGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAAt > 2:279105/1‑139 (MQ=255) tGGCAATAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAAt > 1:1272553/1‑139 (MQ=255) aaTAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGc > 2:884642/1‑77 (MQ=255) aaTAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGc < 1:884642/77‑1 (MQ=255) aaTAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCt < 2:510655/139‑1 (MQ=255) aaTAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCt < 1:597178/139‑1 (MQ=255) tAACGTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGt > 1:461068/1‑139 (MQ=255) gTTTTTCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGt < 2:435356/139‑1 (MQ=255) tttCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCa < 2:494299/97‑1 (MQ=255) tttCACCGCACCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCa > 1:494299/1‑97 (MQ=255) | CAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGT > NC_000913/3782950‑3783212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |