Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,991	G→A	*272* (TAG→TAA)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,991	0	G	A	100.0%	296.1 / NA	89	*272* (TAG→TAA)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/44);  total (45/44)

TCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344863‑345125                                                                                                                                 |                                                                                                                                       tctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAgg                                                                                                                              >  2:1205611/1‑139 (MQ=255)   tGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTGAATGAATGGATATCTAATAGAGTGCACGAATTAGGTGACAGATATTATCCTGTAAATATGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGt                                                                                                                            <  1:1054327/139‑1 (MQ=255)       cTGGACGTAAAAACATACAGACATGATGCGCGATTAAATGAATGGA‑ATCTAATAGAGTGCAGGAATTAGCTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCAc                                                                                                                       <  2:1008514/139‑1 (MQ=255)               aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAg                                                                                                                >  1:1248028/1‑139 (MQ=255)                aaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgt                                                                                                               <  1:455746/139‑1 (MQ=255)                 aaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                              >  1:312678/1‑139 (MQ=255)                   aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                            <  2:178907/139‑1 (MQ=255)                     aTACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                                          <  2:312678/139‑1 (MQ=255)                       aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        <  1:1205611/139‑1 (MQ=255)                             atgatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCt                                                                                                  >  2:1612746/1‑139 (MQ=255)                                atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgag                                                                                               >  2:227372/1‑139 (MQ=255)                                    gcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:1258892/139‑1 (MQ=255)                                     cgATTAAATGAATGTATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGa                                                                                          >  1:1256866/1‑139 (MQ=255)                                       aTTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        >  2:115823/1‑139 (MQ=255)                                           aatgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                                                    >  1:936579/1‑139 (MQ=255)                                           aatgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                                                    <  2:936579/139‑1 (MQ=255)                                            atgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   <  1:227372/139‑1 (MQ=255)                                                    atatCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                           <  1:1427547/139‑1 (MQ=255)                                                              gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                       >  2:1004323/1‑101 (MQ=255)                                                              gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                       <  1:1004323/101‑1 (MQ=255)                                                                gTGCAGGAATTAGGTGACAGATATTGTCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        <  1:1098201/114‑1 (MQ=255)                                                                gTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        >  2:1098201/1‑114 (MQ=255)                                                                gTGCAGGAATTAGGTGAAAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatct                                                               >  1:1084861/1‑139 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCa                                                                                                                        <  1:1308601/81‑1 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCa                                                                                                                        >  2:1308601/1‑81 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                      <  2:123025/115‑1 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                      >  1:123025/1‑115 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATg                                                                                 <  2:578173/120‑1 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATg                                                                                 >  1:578173/1‑120 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                              >  2:1075356/1‑139 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                              >  1:1162456/1‑139 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                              >  2:666630/1‑139 (MQ=255)                                                                 tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                              <  1:1075356/139‑1 (MQ=255)                                                                  gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   <  1:1184815/117‑1 (MQ=255)                                                                  gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   >  2:1184815/1‑117 (MQ=255)                                                                  gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                             >  2:946079/1‑139 (MQ=255)                                                                   cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            <  1:946079/139‑1 (MQ=255)                                                                   cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            <  1:1612746/139‑1 (MQ=255)                                                                                aCAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                                                     >  2:935217/1‑101 (MQ=255)                                                                                aCAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                                                     <  1:935217/101‑1 (MQ=255)                                                                                aCAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa                                               <  2:1256866/139‑1 (MQ=255)                                                                                 cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGc                                                                                >  2:247373/1‑105 (MQ=255)                                                                                 cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGc                                                                                <  1:247373/105‑1 (MQ=255)                                                                                  aGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAc                                             <  2:1084861/139‑1 (MQ=255)                                                                                    atatTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAttt                                                                    <  1:1148736/114‑1 (MQ=255)                                                                                    atatTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAttt                                                                    >  2:1148736/1‑114 (MQ=255)                                                                                      attatCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGc                                         >  1:357501/1‑139 (MQ=255)                                                                                       ttatCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCTCCTGAGAGTGAATCGCAATGCAGGTa                                                                           <  1:1419839/104‑1 (MQ=255)                                                                                       ttatCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa                                                                           >  2:1419839/1‑104 (MQ=255)                                                                                          tCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                           >  2:1520306/1‑53 (MQ=255)                                                                                          tCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                           <  1:1520306/53‑1 (MQ=255)                                                                                          tCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        <  1:1011833/88‑1 (MQ=255)                                                                                          tCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        >  2:1011833/1‑88 (MQ=255)                                                                                          tCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTc                                     <  2:520314/139‑1 (MQ=255)                                                                                             tGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                                  >  1:1492119/1‑43 (MQ=255)                                                                                             tGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGa                                                                                                                                  <  2:1492119/43‑1 (MQ=255)                                                                                              gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  2:1296250/105‑1 (MQ=255)                                                                                              gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  1:1296250/1‑105 (MQ=255)                                                                                               tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                                >  1:877593/1‑139 (MQ=255)                                                                                               tAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                                >  2:497014/1‑139 (MQ=255)                                                                                                aaaTAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtctc                               >  1:1672017/1‑139 (MQ=255)                                                                                                    aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAg                                                                              >  2:1646215/1‑88 (MQ=255)                                                                                                    aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAg                                                                              <  1:1646215/88‑1 (MQ=255)                                                                                                    aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           <  1:475127/107‑1 (MQ=255)                                                                                                    aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           >  2:475127/1‑107 (MQ=255)                                                                                                    aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                                >  2:1702826/1‑118 (MQ=255)                                                                                                    aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                                <  1:1702826/118‑1 (MQ=255)                                                                                                    aaGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttt                           >  1:1207756/1‑139 (MQ=255)                                                                                                      gCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttttt                         <  2:1248028/139‑1 (MQ=255)                                                                                                         cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCaa                                                                                                                 >  1:1214883/1‑48 (MQ=255)                                                                                                         cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCaa                                                                                                                 <  2:1214883/48‑1 (MQ=255)                                                                                                         cGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGc                      >  1:1593817/1‑139 (MQ=255)                                                                                                           ttAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCg                    <  1:115823/139‑1 (MQ=255)                                                                                                           ttAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCg                    <  2:357501/139‑1 (MQ=255)                                                                                                           ttAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCg                    >  1:1418532/1‑139 (MQ=255)                                                                                                           ttAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCg                    <  2:1418532/139‑1 (MQ=255)                                                                                                                        cacTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  1:1510320/1‑55 (MQ=255)                                                                                                                        cacTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  2:1510320/55‑1 (MQ=255)                                                                                                                        cacTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  2:939192/55‑1 (MQ=255)                                                                                                                        cacTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  1:939192/1‑55 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                       >  2:58922/1‑38 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTc                                                                                                       <  1:58922/38‑1 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:1415033/50‑1 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  2:1415033/1‑50 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                      >  1:14878/1‑55 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                      <  2:14878/55‑1 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           <  2:840214/82‑1 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           >  1:840214/1‑82 (MQ=255)                                                                                                                             tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCgg  >  2:524490/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       TCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344863‑345125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑