Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,811 C→T intergenic (‑43/+23) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8110CT100.0% 173.4 / NA 53intergenic (‑43/+23)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

GGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCC  >  NC_000913/3815675‑3815946
                                                                                                                                        |                                                                                                                                       
ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGa                                                                                                                                       >  1:180527/1‑139 (MQ=255)
    tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACtca                                                                                                                                   >  2:620646/1‑139 (MQ=255)
         gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGt                                                                                                                              >  1:487003/1‑139 (MQ=255)
         gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGt                                                                                                                              <  1:989718/139‑1 (MQ=255)
          cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTc                                                                                                                             >  2:1466724/1‑139 (MQ=255)
              gATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCt                                                                                                                         <  2:487003/139‑1 (MQ=255)
                                ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAg                                                                                                       >  2:350678/1‑139 (MQ=255)
                                    aCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGGTTGCCAGcgcc                                                                                                   >  1:1123354/1‑139 (MQ=255)
                                    aCTTTAACACCTGCTTGCTAAGCCCAAATTCAATAAACTGGCGCTGATATTGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                   <  1:1466724/139‑1 (MQ=255)
                                            acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                           <  2:235685/139‑1 (MQ=255)
                                            acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                           <  2:192545/139‑1 (MQ=255)
                                                        aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAAt                                                                               >  1:625100/1‑139 (MQ=255)
                                                           gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGga                                                                            >  1:1717956/1‑139 (MQ=255)
                                                             aaaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAATGTGTCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgatt                                                                          <  2:207053/139‑1 (MQ=255)
                                                                    aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGt                                                                                                                              >  1:1378664/1‑80 (MQ=255)
                                                                    aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGt                                                                                                                              <  2:1378664/80‑1 (MQ=255)
                                                                       aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                          <  1:1541029/97‑1 (MQ=255)
                                                                       aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                          >  2:1541029/1‑97 (MQ=255)
                                                                       aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                                     <  2:1347399/102‑1 (MQ=255)
                                                                       aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                                     >  1:1347399/1‑102 (MQ=255)
                                                                       aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                                <  2:1717956/139‑1 (MQ=255)
                                                                                   tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGcc                                                    <  2:180527/139‑1 (MQ=255)
                                                                                         ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                     >  1:866792/1‑116 (MQ=255)
                                                                                         ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                     <  2:866792/116‑1 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGt                                                                                                                              >  2:1629997/1‑56 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGt                                                                                                                              <  1:1629997/56‑1 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCg                                                                                                                            >  1:615793/1‑58 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCg                                                                                                                            <  2:615793/58‑1 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                          >  1:485115/1‑76 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                          <  2:485115/76‑1 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                           >  2:154800/1‑139 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACGTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgatt                                                                          >  1:1038262/1‑108 (MQ=255)
                                                                                            aTGCCTTCGCTCCTCATCTTACGTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgatt                                                                          <  2:1038262/108‑1 (MQ=255)
                                                                                             tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGa                                          >  1:1623197/1‑139 (MQ=255)
                                                                                                cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                                                                             <  2:1112470/85‑1 (MQ=255)
                                                                                                cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                                                                             >  1:1112470/1‑85 (MQ=255)
                                                                                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                   <  1:564172/78‑1 (MQ=255)
                                                                                                 ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                   >  2:564172/1‑78 (MQ=255)
                                                                                                   cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGACTAGct                                    >  1:352750/1‑139 (MQ=255)
                                                                                                              ttACTTTTCTACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                         <  1:691509/139‑1 (MQ=255)
                                                                                                                       tACAGACAAAAAAAAGGTGCCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCggg                                                            <  1:1043371/95‑1 (MQ=255)
                                                                                                                       tACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                   >  2:962770/1‑56 (MQ=255)
                                                                                                                       tACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                   <  1:962770/56‑1 (MQ=255)
                                                                                                                       tACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAAt                                                                               >  2:653401/1‑76 (MQ=255)
                                                                                                                       tACAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAAt                                                                               <  1:653401/76‑1 (MQ=255)
                                                                                                                       tACAGACAAAAAAAAGGTGACGCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCATCggg                                                            >  2:1043371/1‑95 (MQ=255)
                                                                                                                         cAGACAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc              >  1:538610/1‑139 (MQ=255)
                                                                                                                            aCAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                        >  1:759378/1‑62 (MQ=255)
                                                                                                                            aCAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                        <  2:759378/62‑1 (MQ=255)
                                                                                                                            aCAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                      >  1:1642433/1‑112 (MQ=255)
                                                                                                                            aCAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                      <  2:1642433/112‑1 (MQ=255)
                                                                                                                              aaaaaaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg         >  2:1008066/1‑139 (MQ=255)
                                                                                                                                aaaaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc       <  1:1008066/139‑1 (MQ=255)
                                                                                                                                     aGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc  <  1:620646/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCC  >  NC_000913/3815675‑3815946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑