Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,852,890	C→T	*33* (TAG→TAA)	ivbL ←	ilvB operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,852,890	0	C	T	100.0%	108.8 / NA	34	*33* (TAG→TAA)	ivbL	ilvB operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

TGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATAT  >  NC_000913/3852767‑3853021                                                                                                                            |                                                                                                                                    tgtgCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcg                                                                                                                      <  2:1419149/139‑1 (MQ=255) tgtgCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcg                                                                                                                      <  1:398102/139‑1 (MQ=255)  gtgCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcga                                                                                                                     <  1:1716006/139‑1 (MQ=255)   tgCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgac                                                                                                                    >  2:1048148/1‑139 (MQ=255)     cccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgacga                                                                                                                  >  2:390532/1‑139 (MQ=255)     cccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgacga                                                                                                                  >  1:610156/1‑139 (MQ=255)          aCTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcg                                                                                                                      >  1:1375035/1‑130 (MQ=255)          aCTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcg                                                                                                                      <  2:1375035/130‑1 (MQ=255)                  tGCTCCAGACCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGAGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCAcacg                                                                                                     <  1:1028919/139‑1 (MQ=255)                   gCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCAcacgc                                                                                                    <  2:610156/139‑1 (MQ=255)                         gTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCacgacg                                                                                              >  1:830069/1‑139 (MQ=255)                          tCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTAAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCAcgacga                                                                                             <  1:1048148/139‑1 (MQ=255)                                      gggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCa                                                                                          <  1:1497750/130‑1 (MQ=255)                                      gggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCa                                                                                          >  2:1497750/1‑130 (MQ=255)                                       ggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGTGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGa                                                                                >  2:422157/1‑139 (MQ=255)                                                      aGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCgacgac                                                                                                                 >  2:90358/1‑91 (MQ=255)                                                      aGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCgacgac                                                                                                                 <  1:90358/91‑1 (MQ=255)                                                            ttAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcg                                                                                                                      >  2:58315/1‑80 (MQ=255)                                                            ttAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcg                                                                                                                      <  1:58315/80‑1 (MQ=255)                                                                aCGAAAAACCCCGCCCGGTTTGTGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCg                                                       <  1:422157/139‑1 (MQ=255)                                                                aCGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCg                                                       <  2:830069/139‑1 (MQ=255)                                                                  gAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCacgac                                                                                               >  2:1361789/1‑97 (MQ=255)                                                                  gAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCacgac                                                                                               <  1:1361789/97‑1 (MQ=255)                                                                    aaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCa                                                                                          <  1:788131/100‑1 (MQ=255)                                                                    aaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCa                                                                                          >  2:788131/1‑100 (MQ=255)                                                                                            cggGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTa                                                                                                                                   <  1:324016/35‑1 (MQ=255)                                                                                             ggggTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCt                          <  1:574762/139‑1 (MQ=255)                                                                                                gTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttgt                       <  1:451386/139‑1 (MQ=255)                                                                                                     ggAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGtt                  <  1:390532/139‑1 (MQ=255)                                                                                                          cGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagt                                         <  1:596640/111‑1 (MQ=255)                                                                                                          cGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagt                                         >  2:596640/1‑111 (MQ=255)                                                                                                                   ttCCAGTCCTTACGGCGCATTGCCGACGACCACCACCacac                                                                                                      >  2:1659844/1‑41 (MQ=255)                                                                                                                   ttCCAGTCCTTACGGCGCATTGCCGACGACCACCACCacac                                                                                                      <  1:1659844/41‑1 (MQ=255)                                                                                                                     ccAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAatat  >  2:1734565/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                    TGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATAT  >  NC_000913/3852767‑3853021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑