Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,855,960 C→T S4N (AGC→AAC) 
*116* (TAG→TAA) 		 yidG ←
yidH ←		 inner membrane protein
DUF202 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,855,9600CT100.0% 224.9 / NA 68S4N (AGC→AAC) 
*116* (TAG→TAA) 		yidG
yidH		inner membrane protein
DUF202 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/36);  total (32/36)

AACATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGC  >  NC_000913/3855825‑3856090
                                                                                                                                       |                                                                                                                                  
aaCATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTAt                                                                                                                                 >  1:161213/1‑139 (MQ=255)
 aCATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                <  2:1534873/139‑1 (MQ=255)
   aTGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCg                                                                                                                              <  2:637659/139‑1 (MQ=255)
    tGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                             <  2:1569831/139‑1 (MQ=255)
         gCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATac                                                                                                                        <  1:284627/139‑1 (MQ=255)
          ccTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATaca                                                                                                                       >  1:714343/1‑139 (MQ=255)
          catGGTGCCAGTTGTGTTTGAAAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATaca                                                                                                                       >  2:1042441/3‑139 (MQ=255)
           cTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATacaa                                                                                                                      <  2:108993/139‑1 (MQ=255)
              gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   >  2:1530480/1‑139 (MQ=255)
              gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   <  2:161213/139‑1 (MQ=255)
               tGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACAcc                                                                                                                  >  2:469043/1‑139 (MQ=255)
                        tgtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                <  2:488389/116‑1 (MQ=255)
                        tgtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                >  1:488389/1‑116 (MQ=255)
                        tgtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATa                                                                                                         >  2:708476/1‑139 (MQ=255)
                         gtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   >  2:912165/1‑128 (MQ=255)
                         gtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   <  1:912165/128‑1 (MQ=255)
                           ttGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                             >  1:305816/1‑116 (MQ=255)
                           ttGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                             <  2:305816/116‑1 (MQ=255)
                            tGATAGCCAACGCCATCAACGCGCCGTAG‑CCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGAt                                                                                                    <  1:582593/139‑1 (MQ=255)
                              aTAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATc                                                                                                   <  1:708476/139‑1 (MQ=255)
                                  ccAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   >  2:1326973/1‑119 (MQ=255)
                                  ccAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   <  1:1326973/119‑1 (MQ=255)
                                      cGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATATAACACCAGTCCCATAACGATCACCGCCa                                                                                            >  1:1173862/1‑138 (MQ=255)
                                      cGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCa                                                                                            <  2:1173862/138‑1 (MQ=255)
                                            cAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                     <  1:1530480/139‑1 (MQ=255)
                                                   ccGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGcc                                                                                             >  2:1734024/1‑124 (MQ=255)
                                                   ccGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGcc                                                                                             <  1:1734024/124‑1 (MQ=255)
                                                       aGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                     >  1:473343/1‑128 (MQ=255)
                                                       aGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                     <  2:473343/128‑1 (MQ=255)
                                                                   tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   >  1:575177/1‑86 (MQ=255)
                                                                   tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   <  2:575177/86‑1 (MQ=255)
                                                                   tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATtaata                                                              >  2:1723627/1‑139 (MQ=255)
                                                                    aCGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATtaataa                                                             <  2:856517/139‑1 (MQ=255)
                                                                        aaCCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTg                                                         <  1:1723627/139‑1 (MQ=255)
                                                                         aCCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTCATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                        <  1:1042441/139‑1 (MQ=255)
                                                                                        tgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATtaataa                                                             <  1:1519615/119‑1 (MQ=255)
                                                                                        tgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATtaataa                                                             >  2:1519615/1‑119 (MQ=255)
                                                                                        tgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                        <  1:1016526/124‑1 (MQ=255)
                                                                                        tgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                        >  2:1016526/1‑124 (MQ=255)
                                                                                        tgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGt                                         >  2:524329/1‑139 (MQ=255)
                                                                                         gcgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                >  2:1171099/1‑51 (MQ=255)
                                                                                         gcgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                <  1:1171099/51‑1 (MQ=255)
                                                                                         gcgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTc                                        <  1:524329/139‑1 (MQ=255)
                                                                                          cgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCg                                                                                                                              >  1:125192/1‑52 (MQ=255)
                                                                                          cgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCg                                                                                                                              <  2:125192/52‑1 (MQ=255)
                                                                                          cgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                          >  1:322344/1‑88 (MQ=255)
                                                                                          cgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                          <  2:322344/88‑1 (MQ=255)
                                                                                            cTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGAt                                                                                                    >  1:1269951/1‑76 (MQ=255)
                                                                                            cTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGAt                                                                                                    <  2:1269951/76‑1 (MQ=255)
                                                                                             tCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATaa                                                                                                        >  2:478884/1‑71 (MQ=255)
                                                                                             tCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATaa                                                                                                        <  1:478884/71‑1 (MQ=255)
                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   >  1:477050/1‑58 (MQ=255)
                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   <  2:477050/58‑1 (MQ=255)
                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATa                                                                                                         <  1:724484/68‑1 (MQ=255)
                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATa                                                                                                         >  2:724484/1‑68 (MQ=255)
                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                          >  1:1676840/1‑83 (MQ=255)
                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                          <  2:1676840/83‑1 (MQ=255)
                                                                                                            gggTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCt                     >  1:1662410/1‑139 (MQ=255)
                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   <  1:1694346/39‑1 (MQ=255)
                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                   >  2:1694346/1‑39 (MQ=255)
                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAg                                                                                                                <  1:915402/42‑1 (MQ=255)
                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAg                                                                                                                >  2:915402/1‑42 (MQ=255)
                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCa                                                                                                           <  1:1533666/47‑1 (MQ=255)
                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCa                                                                                                           >  2:1533666/1‑47 (MQ=255)
                                                                                                                    aaTGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCAtt             >  2:409705/1‑139 (MQ=255)
                                                                                                                       gCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                     >  1:882374/1‑64 (MQ=255)
                                                                                                                       gCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                     <  2:882374/64‑1 (MQ=255)
                                                                                                                               cTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAgc  <  1:469043/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
AACATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGC  >  NC_000913/3855825‑3856090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑