Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,908,514	A→G	intergenic (‑148/+35)	pstB ← / ← pstA	phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,908,514	0	A	G	100.0%	173.2 / NA	48	intergenic (‑148/+35)	pstB/pstA	phosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

CTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAA  >  NC_000913/3908379‑3908641                                                                                                                                        |                                                                                                                                cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCAT‑CGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGt                                                                                                                             <  2:56287/139‑1 (MQ=255)      cGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAgg                                                                                                                         >  2:184132/1‑139 (MQ=255)                        cTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAATTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgtgt                                                                                                       <  2:1388665/139‑1 (MQ=255)                        cTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTa                                                                                                                           >  2:1311365/1‑119 (MQ=255)                        cTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTa                                                                                                                           <  1:1311365/119‑1 (MQ=255)                        cTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgtgt                                                                                                       >  1:1388665/1‑139 (MQ=255)                                gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAata                                                                                               >  2:137745/1‑139 (MQ=255)                                     ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgtgt                                                                                                       >  1:1517081/1‑126 (MQ=255)                                     ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgtgt                                                                                                       <  2:1517081/126‑1 (MQ=255)                                     ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                          <  2:1319731/139‑1 (MQ=255)                                          ggCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgt                                                                                                         >  1:134187/1‑119 (MQ=255)                                          ggCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgt                                                                                                         <  2:134187/119‑1 (MQ=255)                                             cGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAg                                                                                                    <  2:923885/121‑1 (MQ=255)                                             cGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAg                                                                                                    >  1:923885/1‑121 (MQ=255)                                               gATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGATTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGt                                                                                                                       <  1:1171022/100‑1 (MQ=255)                                               gATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGt                                                                                                                       >  2:1171022/1‑100 (MQ=255)                                               gATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAtt                                                                                >  2:1343935/1‑139 (MQ=255)                                                     gCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGc                                                                          >  1:1538555/1‑139 (MQ=255)                                                          cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaaa                                                                     >  1:1719916/1‑139 (MQ=255)                                                                      ggCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCa                                                         <  1:1343935/139‑1 (MQ=255)                                                                                 aaCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCACAAACAACGCGCGCCAGACTGTTcagc                                              >  2:1054549/1‑139 (MQ=255)                                                                                       gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTa                                                                                                                           <  1:281029/56‑1 (MQ=255)                                                                                       gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTa                                                                                                                           >  2:281029/1‑56 (MQ=255)                                                                                       gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGt                                        >  2:402113/1‑139 (MQ=255)                                                                                             agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                   <  2:1252689/74‑1 (MQ=255)                                                                                             agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                   >  1:1138261/1‑74 (MQ=255)                                                                                             agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                   <  2:1138261/74‑1 (MQ=255)                                                                                             agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                   >  1:1252689/1‑74 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAgg                                                                                                                         >  1:708176/1‑51 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAgg                                                                                                                         <  2:708176/51‑1 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGaaa                                                                                                              >  1:905801/1‑62 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGaaa                                                                                                              <  2:905801/62‑1 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                          <  1:1688704/82‑1 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                          >  2:1688704/1‑82 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca                                 <  1:137745/139‑1 (MQ=255)                                                                                               tCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                          <  2:980679/81‑1 (MQ=255)                                                                                               tCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                          >  1:980679/1‑81 (MQ=255)                                                                                                         gcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                                  <  2:337260/111‑1 (MQ=255)                                                                                                         gcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                                  >  1:337260/1‑111 (MQ=255)                                                                                                               tcggtcCCCTCGCCCCTCTGGGAAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAcc                <  2:1685906/139‑1 (MQ=255)                                                                                                                  gtcCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCg             >  1:122074/1‑139 (MQ=255)                                                                                                                     cccTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                               >  2:726211/1‑70 (MQ=255)                                                                                                                     cccTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                               <  1:726211/70‑1 (MQ=255)                                                                                                                         cGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttctt                                                                             >  2:697909/1‑68 (MQ=255)                                                                                                                         cGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttctt                                                                             <  1:697909/68‑1 (MQ=255)                                                                                                                          gCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc     <  1:184132/139‑1 (MQ=255)                                                                                                                          gCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTCTCAGCAATATCAACCGTGTTTATTCTACGCAAAAACAACGCGCGCCAGAATGTTCAGCGGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc     <  1:1054549/139‑1 (MQ=255)                                                                                                                             ccTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCaa  >  2:1462811/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                CTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAA  >  NC_000913/3908379‑3908641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑