| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,920,950 | C→T | *80* (TAG→TAA) | atpE ← | F0 sector of membrane‑bound ATP synthase, subunit c |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,920,950 | 0 | C | T | 98.5% | 212.2 / NA | 66 | *80* (TAG→TAA) | atpE | F0 sector of membrane‑bound ATP synthase, subunit c |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (33/32); minor base G (1/0); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCCAG > NC_000913/3920823‑3921084 | cATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGc > 1:774398/1‑139 (MQ=255)cATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGc > 2:1714468/1‑139 (MQ=255) tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg > 1:1232856/1‑130 (MQ=255) tGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg < 2:1232856/130‑1 (MQ=255) acgaacAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc < 2:774398/139‑1 (MQ=255) aCAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacccaga > 1:1083640/1‑139 (MQ=255) aTGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg < 2:145618/101‑1 (MQ=255) aTGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcg > 1:145618/1‑101 (MQ=255) ctggccGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatcat < 1:1714468/139‑1 (MQ=255) cGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa < 1:1493213/139‑1 (MQ=255) ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAg > 2:1063300/1‑139 (MQ=255) aTTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTCTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa < 1:1063300/139‑1 (MQ=255) ttgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATcca > 2:215307/1‑139 (MQ=255) ttAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGa < 1:215307/139‑1 (MQ=255) caATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAg > 2:1427527/1‑139 (MQ=255) tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGAcc < 2:482108/123‑1 (MQ=255) tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGAcc > 1:482108/1‑123 (MQ=255) tAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc > 1:1469889/1‑69 (MQ=255) tAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc < 2:1469889/69‑1 (MQ=255) aGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATaaa > 1:852091/1‑124 (MQ=255) aGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATaaa < 2:852091/124‑1 (MQ=255) ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATa > 2:1624307/1‑96 (MQ=255) ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATa < 1:1624307/96‑1 (MQ=255) ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCAt < 1:1530779/100‑1 (MQ=255) ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCAt > 2:1530779/1‑100 (MQ=255) gTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGa > 1:360404/1‑139 (MQ=255) gTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGa > 2:59262/1‑139 (MQ=255) gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacca < 2:1210436/90‑1 (MQ=255) gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacca > 1:1210436/1‑90 (MQ=255) gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc < 1:494082/89‑1 (MQ=255) gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc > 2:494082/1‑89 (MQ=255) gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGcagcag < 1:1723062/124‑1 (MQ=255) gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGcagcag > 2:1723062/1‑124 (MQ=255) gCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAgg > 2:758684/1‑139 (MQ=255) ctctTTAAATAAAAGCAACGCTTATTACGCGACAGc < 1:574877/36‑1 (MQ=255) ctctTTAAATAAAAGCAACGCTTATTACGCGACAGc > 2:574877/1‑36 (MQ=255) ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCTAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGa < 1:1173126/107‑1 (MQ=255) ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGa > 1:1182004/1‑107 (MQ=255) ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACACCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGa < 2:1182004/107‑1 (MQ=255) ctctTTAAATAAAAGCAACGCTTAGTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGa > 2:1173126/1‑107 (MQ=255) ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc > 2:565360/1‑50 (MQ=255) ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc < 1:565360/50‑1 (MQ=255) ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc > 1:741865/1‑45 (MQ=255) ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc < 2:741865/45‑1 (MQ=255) taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc > 2:499894/1‑49 (MQ=255) taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc < 1:499894/49‑1 (MQ=255) taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGAcg > 1:354488/1‑139 (MQ=255) aaGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg < 1:1427527/139‑1 (MQ=255) aaGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg < 1:758684/139‑1 (MQ=255) aGCAACGCTTATTACGCGACAGCGAACATCACGTa > 1:1267241/1‑35 (MQ=255) aGCAACGCTTATTACGCGACAGCGAACATCACGTa < 2:1267241/35‑1 (MQ=255) aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc > 2:812398/1‑75 (MQ=255) aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc < 1:812398/75‑1 (MQ=255) aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCg < 2:1720576/50‑1 (MQ=255) aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCg > 1:1720576/1‑50 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTAcagacc < 1:330923/36‑1 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTAcagacc > 2:330923/1‑36 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTATAGACCCAGACCTACa < 2:1155655/46‑1 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACa > 1:1155655/1‑46 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc < 1:1286453/70‑1 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc > 2:1286453/1‑70 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt > 1:1214263/1‑92 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt < 2:1214263/92‑1 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg > 1:142224/1‑133 (MQ=255) cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg < 2:142224/133‑1 (MQ=255) cTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCCAg < 2:354488/139‑1 (MQ=255) | CATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCCAG > NC_000913/3920823‑3921084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |