Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,923,070	G→A	A204V (GCA→GTA)	rsmG ←	16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,923,070	0	G	A	100.0%	149.1 / NA	46	A204V (GCA→GTA)	rsmG	16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

ACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCA  >  NC_000913/3922935‑3923190                                                                                                                                        |                                                                                                                         aCGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACtt                                                                                                                       <  2:800019/139‑1 (MQ=255)     tATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAAt                                                                                                                   <  1:744477/139‑1 (MQ=255)                 gCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACtt                                                                                                                       >  1:1001765/1‑123 (MQ=255)                 gCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACtt                                                                                                                       <  2:1001765/123‑1 (MQ=255)                            aaCGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCAt                                                                                            >  2:511483/1‑139 (MQ=255)                                      tAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATcac                                                                                                                <  1:1234444/109‑1 (MQ=255)                                      tAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATcac                                                                                                                >  2:1234444/1‑109 (MQ=255)                                               ccAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAg                                                                         >  1:1533336/1‑139 (MQ=255)                                                           gTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGa                                                             >  2:1270568/1‑139 (MQ=255)                                                            tacaaaaCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTCTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTgg                                                                                  <  1:157279/114‑1 (MQ=255)                                                            tAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            <  1:1270568/139‑1 (MQ=255)                                                            tAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTAAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTgg                                                                                  >  2:157279/1‑117 (MQ=255)                                                                    cTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTg                                                    >  1:322607/1‑139 (MQ=255)                                                                      ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             >  2:852915/1‑112 (MQ=255)                                                                      ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             <  1:852915/112‑1 (MQ=255)                                                                      ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            <  1:707472/129‑1 (MQ=255)                                                                      ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            >  2:707472/1‑129 (MQ=255)                                                                      ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGat                                                  >  1:1558084/1‑139 (MQ=255)                                                                                aaCAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            <  2:896301/119‑1 (MQ=255)                                                                                aaCAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            >  1:896301/1‑119 (MQ=255)                                                                                     tCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                <  1:330966/78‑1 (MQ=255)                                                                                     tCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                >  2:330966/1‑78 (MQ=255)                                                                                     tCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGcaaca                                   >  1:1579248/1‑139 (MQ=255)                                                                                      cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCa                                                                                         <  1:1692994/84‑1 (MQ=255)                                                                                      cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCa                                                                                         >  2:1692994/1‑84 (MQ=255)                                                                                       tAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACaaa                                 <  1:511483/139‑1 (MQ=255)                                                                                       tAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACaaa                                 <  2:1533336/139‑1 (MQ=255)                                                                                        aaCCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                <  1:1494687/75‑1 (MQ=255)                                                                                        aaCCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                >  2:1494687/1‑75 (MQ=255)                                                                                        aaCCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAg                                <  1:1486622/139‑1 (MQ=255)                                                                                             gTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             <  1:1065201/89‑1 (MQ=255)                                                                                             gTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             >  2:1065201/1‑89 (MQ=255)                                                                                               cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGttt                                                                      <  1:959696/94‑1 (MQ=255)                                                                                               cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGttt                                                                      >  2:959696/1‑94 (MQ=255)                                                                                               cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCa                                                                 >  1:996766/1‑99 (MQ=255)                                                                                               cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCa                                                                 <  2:996766/99‑1 (MQ=255)                                                                                               cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTc                         >  1:500355/1‑139 (MQ=255)                                                                                               cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTc                         >  2:889358/1‑139 (MQ=255)                                                                                                aaTTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                >  2:651544/1‑67 (MQ=255)                                                                                                aaTTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                <  1:651544/67‑1 (MQ=255)                                                                                                            tttttttGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCAtt            <  2:1558084/139‑1 (MQ=255)                                                                                                              tttttGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTg          <  2:1579248/139‑1 (MQ=255)                                                                                                               ttttGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACATGATATTCTTCGGGCAACAAAGCGATTTCATATTCCGGCATTTGc         >  2:948333/1‑139 (MQ=255)                                                                                                                      aaaaaTTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCa  <  2:500355/139‑1 (MQ=255)                                                                                                                                     tttACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                       <  2:1097506/71‑1 (MQ=255)                                                                                                                                     tttACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                       >  1:1097506/1‑71 (MQ=255)                                                                                                                                        |                                                                                                                         ACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCA  >  NC_000913/3922935‑3923190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑