Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,965,088 C→T W181* (TGG→TGA)  rhlB ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,965,0880CT83.6% 141.3 / 23.3 67W181* (TGG→TGA) rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base T (27/29);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

TTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATG  >  NC_000913/3964953‑3965223
                                                                                                                                       |                                                                                                                                       
tttCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagtcag                                                                                                                                      >  1:1546386/1‑139 (MQ=255)
    tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGa                                                                                                                                  <  1:467075/139‑1 (MQ=255)
     gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGat                                                                                                                                 <  2:1353119/139‑1 (MQ=255)
     gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                                                                                                 <  2:1100525/139‑1 (MQ=255)
      ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGata                                                                                                                                >  2:408687/1‑139 (MQ=255)
              ccACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTtaat                                                                                                                        <  1:1446641/139‑1 (MQ=255)
               cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTtaata                                                                                                                       <  2:1546386/139‑1 (MQ=255)
                       tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTtaa                                                                                                                         <  1:679681/129‑1 (MQ=255)
                       tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTtaa                                                                                                                         >  2:679681/1‑129 (MQ=255)
                        atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGa                                                                                                              <  1:212/139‑1 (MQ=255)
                        atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                                                                                                              <  1:647404/139‑1 (MQ=255)
                            tCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGa                                                                                                              >  1:1388456/1‑135 (MQ=255)
                            tCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGa                                                                                                              <  2:1388456/135‑1 (MQ=255)
                            tCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGt                                                                                                          >  1:1204177/1‑139 (MQ=255)
                              ggCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTtaat                                                                                                                        >  1:23935/1‑123 (MQ=255)
                              ggCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTtaat                                                                                                                        <  2:23935/123‑1 (MQ=255)
                               gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGAt                                                                                                             >  1:900593/1‑133 (MQ=255)
                               gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGAt                                                                                                             <  2:900593/133‑1 (MQ=255)
                                     ttCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                                                                                                            <  1:1030506/128‑1 (MQ=255)
                                     ttCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                                                                                                            >  2:1030506/1‑128 (MQ=255)
                                          cTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt                                                                                            <  2:349815/139‑1 (MQ=255)
                                             cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg                                                                                         >  2:308532/1‑139 (MQ=255)
                                             cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg                                                                                         >  2:8432/1‑139 (MQ=255)
                                               cGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTc                                                                                       >  2:166053/1‑139 (MQ=255)
                                               cGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTc                                                                                       >  1:1211104/1‑139 (MQ=255)
                                               cGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTc                                                                                       >  1:222291/1‑139 (MQ=255)
                                                  aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCgg                                                                                                                                   <  2:1441957/92‑1 (MQ=255)
                                                  aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCgg                                                                                                                                   >  1:1441957/1‑92 (MQ=255)
                                                       aGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGccc                                                                                                                 <  1:595042/105‑1 (MQ=255)
                                                       aGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGccc                                                                                                                 >  2:595042/1‑105 (MQ=255)
                                                              gTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt                                                                                            >  2:861100/1‑119 (MQ=255)
                                                              gTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt                                                                                            <  1:861100/119‑1 (MQ=255)
                                                              gTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTg                                                                        >  2:1306380/1‑139 (MQ=255)
                                                                  ccGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATg                                                                    <  1:166053/139‑1 (MQ=255)
                                                                   cGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                                                                                                 <  2:567022/77‑1 (MQ=255)
                                                                   cGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                                                                                                 >  1:567022/1‑77 (MQ=255)
                                                                      tACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGat                                                                                                                                 >  2:730463/1‑74 (MQ=255)
                                                                      tACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGat                                                                                                                                 <  1:730463/74‑1 (MQ=255)
                                                                      tACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGcc                                                                                                                  <  1:1102806/89‑1 (MQ=255)
                                                                      tACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGcc                                                                                                                  >  2:1102806/1‑89 (MQ=255)
                                                                      tACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCa                                                                                                                <  1:1594834/91‑1 (MQ=255)
                                                                      tACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCa                                                                                                                >  2:1594834/1‑91 (MQ=255)
                                                                        cGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCg                                                              >  2:1712435/1‑139 (MQ=255)
                                                                          aaaGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAg                                                            >  1:310779/1‑139 (MQ=255)
                                                                           aaGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGaa                                                                      <  1:560191/128‑1 (MQ=255)
                                                                           aaGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGaa                                                                      >  2:560191/1‑128 (MQ=255)
                                                                           aaGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAgg                                                           <  1:408687/139‑1 (MQ=255)
                                                                                 ggCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAAtg                                                     <  1:308532/139‑1 (MQ=255)
                                                                                             cATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGa                                                                                                                                  >  1:1200263/1‑50 (MQ=255)
                                                                                             cATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGa                                                                                                                                  <  2:1200263/50‑1 (MQ=255)
                                                                                               tGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGc                                       <  1:469048/139‑1 (MQ=255)
                                                                                               tGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGc                                       >  2:874960/1‑139 (MQ=255)
                                                                                                    aGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCAcc                                                               <  1:179846/110‑1 (MQ=255)
                                                                                                    aGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCAcc                                                               >  2:179846/1‑110 (MQ=255)
                                                                                                       cgcTGGTTTGCAGGCGGCATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCaa                               >  1:857097/1‑139 (MQ=255)
                                                                                                          tGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTa                            >  2:1057949/1‑139 (MQ=255)
                                                                                                                   ggcggcATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCg                   <  1:8432/139‑1 (MQ=255)
                                                                                                                   ggcggcATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCg                   <  1:1306380/139‑1 (MQ=255)
                                                                                                                    gcggcATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGtt                                                <  2:464508/109‑1 (MQ=255)
                                                                                                                    gcggcATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGtt                                                >  1:464508/1‑109 (MQ=255)
                                                                                                                       gcATACGGCGGAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtggt               <  2:857097/139‑1 (MQ=255)
                                                                                                                       gcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgtggt               <  2:222291/139‑1 (MQ=255)
                                                                                                                              gcggAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGGGATCGGCTTCGTCCAATACCACCACCTGAATGGCACCGAGGTTaa                                                       <  2:999541/92‑1 (MQ=255)
                                                                                                                              gcggAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGaa                                                                      <  2:862738/77‑1 (MQ=255)
                                                                                                                              gcggAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGaa                                                                      >  1:862738/1‑77 (MQ=255)
                                                                                                                              gcggAACAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTaa                                                       >  1:999541/1‑92 (MQ=255)
                                                                                                                                  aaCAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGt                                                          <  2:367551/85‑1 (MQ=255)
                                                                                                                                  aaCAGTCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGt                                                          >  1:367551/1‑85 (MQ=255)
                                                                                                                                    cagccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATg  >  2:163343/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATG  >  NC_000913/3964953‑3965223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑