Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,077	G→A	*349* (TAG→TAA)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,077	0	G	A	100.0%	166.2 / NA	51	*349* (TAG→TAA)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/26);  total (25/26)

ATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTG  >  NC_000913/3969944‑3970201                                                                                                                                        |                                                                                                                               aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                             <  2:241039/139‑1 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                             <  1:1402114/139‑1 (MQ=255)   cGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                                                                           <  1:980476/139‑1 (MQ=255)                           ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGgcgc                                                                                                   >  2:1172229/1‑139 (MQ=255)                           ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGgcgc                                                                                                   >  2:436956/1‑139 (MQ=255)                                    cgcgATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgcct                                                                                          >  1:1521363/1‑137 (MQ=255)                                    cgcgATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                                                                  >  2:1301617/1‑130 (MQ=255)                                    cgcgATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                                                                  <  1:1301617/130‑1 (MQ=255)                                        aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTc                                                                                      >  2:194422/1‑139 (MQ=255)                                                 gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGTTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAAt                                                                           <  1:1097/139‑1 (MQ=255)                                                 gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCCTATTCGA‑‑AGAGAAt                                                                           <  2:1521363/139‑1 (MQ=255)                                                    gtGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGa                                                                        <  2:411588/139‑1 (MQ=255)                                                     tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                             >  2:574112/1‑119 (MQ=255)                                                     tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                             <  1:574112/119‑1 (MQ=255)                                                     tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGAt                                                                       >  1:1563627/1‑139 (MQ=255)                                                      gCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                             <  2:800636/86‑1 (MQ=255)                                                      gCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                             >  1:800636/1‑86 (MQ=255)                                                                  atTATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTactga                                                          <  1:194422/139‑1 (MQ=255)                                                                   ttATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTactgac                                                         <  1:1172229/139‑1 (MQ=255)                                                                    tATGCGGGGCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTactgact                                                        <  1:436956/139‑1 (MQ=255)                                                                         ggggCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCTGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑aga                                                                               >  2:1243445/1‑111 (MQ=255)                                                                         ggggCATTGTCGGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑aga                                                                               <  1:1243445/111‑1 (MQ=255)                                                                              attgtcggggggggCGGATCGGTGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                              <  2:1563627/131‑1 (MQ=255)                                                                                     gggggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGccc                                       >  1:633483/2‑139 (MQ=255)                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                                                                               <  1:822639/80‑1 (MQ=255)                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                                                                               >  2:822639/1‑80 (MQ=255)                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa                                   >  1:612784/1‑138 (MQ=255)                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa                                   <  2:612784/138‑1 (MQ=255)                                                                                           gCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                             >  2:955746/1‑49 (MQ=255)                                                                                           gCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                             <  1:955746/49‑1 (MQ=255)                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                                                                               >  1:628539/1‑78 (MQ=255)                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                                                                               <  2:628539/78‑1 (MQ=255)                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                                                                                     <  1:940849/88‑1 (MQ=255)                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg                                                                                     >  2:940849/1‑88 (MQ=255)                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGcc                                        <  1:1240407/131‑1 (MQ=255)                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGcc                                        >  2:1240407/1‑131 (MQ=255)                                                                                              gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCgg                                     >  1:659667/1‑132 (MQ=255)                                                                                              gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCgg                                     <  2:659667/132‑1 (MQ=255)                                                                                                     gCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATg                       >  1:857172/1‑139 (MQ=255)                                                                                                      cTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATgg                      <  2:633483/139‑1 (MQ=255)                                                                                                         gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa                                    <  2:1421175/122‑1 (MQ=255)                                                                                                         gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa                                    >  1:1421175/1‑122 (MQ=255)                                                                                                              gCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTactga                                                          <  2:281830/95‑1 (MQ=255)                                                                                                              gCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTactga                                                          >  1:281830/1‑95 (MQ=255)                                                                                                                   aaCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCa         >  1:294687/1‑139 (MQ=255)                                                                                                                     cccGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGa                                                                   >  1:1318123/1‑79 (MQ=255)                                                                                                                     cccGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGa                                                                   <  2:1318123/79‑1 (MQ=255)                                                                                                                          cgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGATGAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa                                    <  2:44237/107‑1 (MQ=255)                                                                                                                          cgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGATGAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa                                    >  1:44237/1‑107 (MQ=255)                                                                                                                          cgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTg  >  1:1048164/1‑139 (MQ=255)                                                                                                                               cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTactgact                                                        <  1:1709363/80‑1 (MQ=255)                                                                                                                               cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTactgact                                                        >  2:1709363/1‑80 (MQ=255)                                                                                                                                        |                                                                                                                               ATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGA‑‑AGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTG  >  NC_000913/3969944‑3970201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑