Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,077,469	G→A	L7L (TTG→TTA)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,077,469	0	G	A	100.0%	215.3 / NA	66	L7L (TTG→TTA)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/30);  total (36/30)

TATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAAGGTTCGGAACGC  >  NC_000913/4077336‑4077604                                                                                                                                      |                                                                                                                                        tATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                    <  1:452443/139‑1 (MQ=255) tATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                    >  2:452443/1‑139 (MQ=255) tATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                    <  1:524006/139‑1 (MQ=255)                     tCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCg                                                                                                                <  1:400327/139‑1 (MQ=255)                      cAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               <  1:1278743/139‑1 (MQ=255)                      cAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               <  2:1339376/139‑1 (MQ=255)                         cAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTAt                                                                                                                  >  2:178658/1‑133 (MQ=255)                         cAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTAt                                                                                                                  <  1:178658/133‑1 (MQ=255)                               aTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCa                                                                                                      >  1:1386859/1‑139 (MQ=255)                               aTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCa                                                                                                      >  2:103531/1‑139 (MQ=255)                                 gAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               <  2:844918/128‑1 (MQ=255)                                 gAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               >  1:844918/1‑128 (MQ=255)                                  aaCACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCt                                                                                                   >  2:1307757/1‑139 (MQ=255)                                       gCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCggg                                                                                              >  1:299888/1‑139 (MQ=255)                                        cAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGt                                                                                             <  1:1236061/139‑1 (MQ=255)                                        cAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGt                                                                                             >  1:523180/1‑139 (MQ=255)                                                 cGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCgg                                                                                               <  1:1215991/128‑1 (MQ=255)                                                 cGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCgg                                                                                               >  2:1215991/1‑128 (MQ=255)                                                 cGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAAc                                                                                    >  1:92005/1‑139 (MQ=255)                                                      cGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                                               <  2:523180/139‑1 (MQ=255)                                                        cTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaagaa                                                                             <  2:1386859/139‑1 (MQ=255)                                                             gATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAAtt                                                                        >  2:767485/1‑139 (MQ=255)                                                              atatGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCg                                                                                                                <  2:573257/98‑1 (MQ=255)                                                              atatGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCg                                                                                                                >  1:573257/1‑98 (MQ=255)                                                                     ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCgg                                                                                               <  1:1492932/108‑1 (MQ=255)                                                                     ggTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCgg                                                                                               >  2:1492932/1‑108 (MQ=255)                                                                        aTCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACt                                                             >  1:291583/1‑139 (MQ=255)                                                                           gCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGa                                                                                                                                 <  1:1579281/68‑1 (MQ=255)                                                                           gCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGa                                                                                                                                 >  2:1579281/1‑68 (MQ=255)                                                                           gCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               <  1:1515855/86‑1 (MQ=255)                                                                           gCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               >  2:1515855/1‑86 (MQ=255)                                                                                    tgatgGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCt                                                 >  2:698020/1‑139 (MQ=255)                                                                                       tgGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                    >  2:1092461/1‑53 (MQ=255)                                                                                       tgGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                    <  1:1092461/53‑1 (MQ=255)                                                                                         gCCGCGTGACATTCTGGTTGCAGCTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGaa                                            >  2:318656/1‑139 (MQ=255)                                                                                          ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                    >  1:538817/1‑50 (MQ=255)                                                                                          ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                                    <  2:538817/50‑1 (MQ=255)                                                                                          ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               >  1:556583/1‑71 (MQ=255)                                                                                          ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                                               <  2:556583/71‑1 (MQ=255)                                                                                          ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACt                                                             >  1:656299/1‑121 (MQ=255)                                                                                          ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACt                                                             <  2:656299/121‑1 (MQ=255)                                                                                             cGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGt                                        <  2:291583/139‑1 (MQ=255)                                                                                              gTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAaga                                                                                                                          <  2:1047423/56‑1 (MQ=255)                                                                                              gTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAaga                                                                                                                          >  1:1047423/1‑56 (MQ=255)                                                                                                   aTTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                                  <  1:698020/139‑1 (MQ=255)                                                                                                     tCTGGTTGCAGGTATGAGCCAGCTTCTAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACa                                                                                   <  1:902294/88‑1 (MQ=255)                                                                                                     tCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACa                                                                                   >  2:902294/1‑88 (MQ=255)                                                                                                     tCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGc                                                  <  1:939350/121‑1 (MQ=255)                                                                                                     tCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGc                                                  >  2:939350/1‑121 (MQ=255)                                                                                                         gTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGcccc                            >  2:422720/1‑139 (MQ=255)                                                                                                         gTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATG‑TGCGTAAGCCCCt                           >  2:327740/1‑139 (MQ=255)                                                                                                             cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCt                                                                                                              >  2:164157/1‑53 (MQ=255)                                                                                                             cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCt                                                                                                              <  1:164157/53‑1 (MQ=255)                                                                                                             cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                                               <  2:312331/84‑1 (MQ=255)                                                                                                             cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaag                                                                               >  1:312331/1‑84 (MQ=255)                                                                                                             cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCa                        >  2:1423710/1‑139 (MQ=255)                                                                                                             cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCa                        >  2:1039927/1‑139 (MQ=255)                                                                                                             cAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCa                        >  2:941315/1‑139 (MQ=255)                                                                                                               ggTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATc                      <  1:1307757/139‑1 (MQ=255)                                                                                                                   tGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAAcc                  >  1:88570/1‑139 (MQ=255)                                                                                                                     aGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCaa                >  1:982965/1‑139 (MQ=255)                                                                                                                     aGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCaa                <  1:1039927/139‑1 (MQ=255)                                                                                                                          gCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTa                                                                       <  2:461977/79‑1 (MQ=255)                                                                                                                          gCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTa                                                                       >  1:461977/1‑79 (MQ=255)                                                                                                                               cAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAAAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAGGGTTCGGa      >  1:1030600/1‑139 (MQ=255)                                                                                                                                   gTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAAGGTTCGGAacgc  <  1:318656/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACCAAAAGGTTCGGAACGC  >  NC_000913/4077336‑4077604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑