| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,499,640 | C→T | pseudogene (895/942 nt) | yjgX ← | pseudogene fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,499,640 | 0 | C | T | 91.3% | 185.2 / 8.0 | 69 | pseudogene (895/942 nt) | yjgX | pseudogene fragment |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (34/29); total (37/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGA > NC_000913/4499537‑4499774 | ttttATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAg > 2:1155670/1‑139 (MQ=255) tttATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAgg < 1:1155670/139‑1 (MQ=255) tataCAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgtt < 2:634305/139‑1 (MQ=255) aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATAtt < 1:1129503/103‑1 (MQ=255) aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATAtt > 2:1129503/1‑103 (MQ=255) aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATc > 2:1454414/1‑139 (MQ=255) tACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGt < 1:1009437/96‑1 (MQ=255) tACTGTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAACTAGCTGAGGTTTCAAACTGTCTGTCGGt > 2:1009437/1‑96 (MQ=255) ttCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGt < 1:1178570/91‑1 (MQ=255) ttCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGt > 2:1178570/1‑91 (MQ=255) tACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCg > 2:1388023/1‑84 (MQ=255) tACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCg < 1:1388023/84‑1 (MQ=255) tACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCctctct < 1:188627/98‑1 (MQ=255) tACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCctctct > 2:188627/1‑98 (MQ=255) tACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAg > 2:508706/1‑139 (MQ=255) tATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATgg < 1:363869/139‑1 (MQ=255) aTTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGc < 1:508706/139‑1 (MQ=255) tCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTa < 1:1395086/84‑1 (MQ=255) tCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTa > 2:1395086/1‑84 (MQ=255) tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCctct < 2:1209133/74‑1 (MQ=255) tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCctct > 1:1209133/1‑74 (MQ=255) tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGaa > 2:895181/1‑126 (MQ=255) tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGaa < 1:895181/126‑1 (MQ=255) tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATAttt > 2:1418239/1‑139 (MQ=255) tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATAttt > 2:528252/1‑139 (MQ=255) gCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATAtttt < 1:1454414/139‑1 (MQ=255) ccTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATAc > 2:819039/1‑137 (MQ=255) ccTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATAc < 1:819039/137‑1 (MQ=255) cTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTa < 1:1063860/66‑1 (MQ=255) cTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTa > 2:1063860/1‑66 (MQ=255) tgtgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGt > 1:304540/1‑139 (MQ=255) tgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgttag < 1:864326/81‑1 (MQ=255) tgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgttag > 2:864326/1‑81 (MQ=255) gATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATg < 2:793351/108‑1 (MQ=255) gATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATg > 1:793351/1‑108 (MQ=255) ggAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATAtt > 1:206342/1‑44 (MQ=255) ggAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATAtt < 2:206342/44‑1 (MQ=255) gaagaaTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgt > 2:1505613/1‑71 (MQ=255) gaagaaTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgt < 1:1505613/71‑1 (MQ=255) gaagaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa > 2:1360183/1‑66 (MQ=255) gaagaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa < 1:1360183/66‑1 (MQ=255) gaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa > 1:858217/1‑63 (MQ=255) gaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa < 2:858217/63‑1 (MQ=255) aaTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGtat < 2:50666/105‑1 (MQ=255) aaTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGtat > 1:50666/1‑105 (MQ=255) aTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTc < 2:717076/84‑1 (MQ=255) aTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTc > 1:717076/1‑84 (MQ=255) aTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATg < 1:1387463/97‑1 (MQ=255) aTAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATg > 2:1387463/1‑97 (MQ=255) tAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCtt > 2:1249692/1‑75 (MQ=255) tAAGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCtt < 1:1249692/75‑1 (MQ=255) aGTGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATAc > 1:217144/1‑139 (MQ=255) tGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCa > 2:1534379/1‑80 (MQ=255) tGGCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCa < 1:1534379/80‑1 (MQ=255) ggCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATaa > 2:1579429/1‑55 (MQ=255) ggCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATaa < 1:1579429/55‑1 (MQ=255) ggCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAGCAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATAc < 2:1189711/136‑1 (MQ=255) ggCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAGCAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATAc > 1:1189711/1‑136 (MQ=255) gCTGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAgg > 2:1063311/1‑58 (MQ=255) gCTGAGATTTCAAACTCTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAga < 1:1063311/58‑2 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCttg > 2:1066849/1‑41 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCttg < 1:1066849/42‑2 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATTTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGa > 1:1212540/1‑139 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGaa < 2:1145018/86‑1 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGaa > 1:1145018/1‑86 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGtat < 2:69758/95‑1 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGtat > 1:69758/1‑95 (MQ=255) tGAGGTTTCAAACTGTCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGa > 1:912923/1‑139 (MQ=255) tCTGTCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGa > 1:553500/1‑139 (MQ=255) | TTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGA > NC_000913/4499537‑4499774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |