Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,549,037 | T→C | V77A (GTC→GCC) | fimH → | minor component of type 1 fimbriae |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,549,037 | 0 | T | C | 96.4% | 189.1 / ‑2.6 | 56 | V77A (GTC→GCC) | fimH | minor component of type 1 fimbriae |
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (27/27); total (28/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 |
CTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGAT > NC_000913/4548905‑4549170 | cTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACt < 2:992963/139‑1 (MQ=255) gTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAgg > 2:1211172/1‑139 (MQ=255) gTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAgg > 1:475153/1‑139 (MQ=255) gTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATggcg < 1:1211172/139‑1 (MQ=255) aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGc < 2:1713099/116‑1 (MQ=255) aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGc > 1:1713099/1‑116 (MQ=255) aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATggcg < 2:232986/129‑1 (MQ=255) aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATggcg > 1:232986/1‑129 (MQ=255) tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAt < 1:1012472/139‑1 (MQ=255) gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAtt > 1:207133/1‑139 (MQ=255) gtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa < 2:207133/139‑1 (MQ=255) ggggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa < 2:309293/131‑1 (MQ=255) ggggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa > 1:309293/1‑131 (MQ=255) gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa > 2:124184/1‑130 (MQ=255) gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa < 1:124184/130‑1 (MQ=255) ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTa > 1:1591230/1‑100 (MQ=255) ggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTa < 2:1591230/100‑1 (MQ=255) ggTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGt > 1:772226/1‑139 (MQ=255) tGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTa > 2:959411/1‑139 (MQ=255) ggATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa < 2:75135/113‑1 (MQ=255) ggATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa > 1:75135/1‑113 (MQ=255) cTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAt < 2:772226/139‑1 (MQ=255) ttCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAAtata > 2:171994/1‑119 (MQ=255) ttCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAAtata < 1:171994/119‑1 (MQ=255) ttCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAttt > 2:325440/1‑139 (MQ=255) ttCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAttt > 1:265176/1‑139 (MQ=255) gACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGc < 2:1520890/67‑1 (MQ=255) gACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGc > 1:1520890/1‑67 (MQ=255) gACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt < 2:475153/139‑1 (MQ=255) gACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt > 1:188756/1‑139 (MQ=255) gACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt < 1:870237/139‑1 (MQ=255) aaaTCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTaccac < 2:265176/139‑1 (MQ=255) aTCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg > 2:1700580/1‑73 (MQ=255) aTCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg < 1:1700580/73‑1 (MQ=255) aaCGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAAcgccgc > 2:1505556/1‑139 (MQ=255) aCGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcg < 1:959411/139‑1 (MQ=255) gATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgcg < 1:1505556/139‑1 (MQ=255) gATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgcg < 1:325440/139‑1 (MQ=255) tATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCgttg > 2:847902/1‑139 (MQ=255) aTCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCgttgt > 1:814345/1‑139 (MQ=255) tCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgcg < 2:853360/134‑1 (MQ=255) tCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgcg > 1:853360/1‑134 (MQ=255) ccGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGttt > 2:682726/1‑139 (MQ=255) ggAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt < 1:1344259/109‑1 (MQ=255) ggAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt > 2:1344259/1‑109 (MQ=255) aaaCCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat > 1:1013593/1‑81 (MQ=255) aaaCCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATaa > 2:928713/1‑139 (MQ=255) aaaCCATTACAGAATATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat < 2:1013593/81‑1 (MQ=255) aCCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGt > 2:1478094/1‑90 (MQ=255) aCCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGt < 1:1478094/90‑1 (MQ=255) ttACAGACTATGCCACACTGCAACGAGGCTCGGCTTATggcg < 2:1452189/42‑1 (MQ=255) ttACAGACTATGCCACACTGCAACGAGGCTCGGCTTATggcg > 1:1452189/1‑42 (MQ=255) aCAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGaa < 1:682726/139‑1 (MQ=255) aCTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTgg > 2:1248162/1‑76 (MQ=255) aCTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTgg < 1:1248162/76‑1 (MQ=255) cTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGAt < 2:188756/139‑1 (MQ=255) | CTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGAT > NC_000913/4548905‑4549170 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |