Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 47,776 | G→A | *177* (TAG→TAA) S3N (AGC→AAC) |
kefF → kefC → |
potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 47,776 | 0 | G | A | 100.0% | 53.2 / NA | 18 | *177* (TAG→TAA) S3N (AGC→AAC) | kefF kefC | potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/8); total (10/8) |
GGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTG > NC_000913/47652‑47884 | ggCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTc > 1:419637/1‑139 (MQ=255) tGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAgg > 2:260190/1‑139 (MQ=255) tGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAgg > 1:303818/1‑139 (MQ=255) cTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGt > 2:1579045/1‑139 (MQ=255) cgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACg > 1:807504/1‑139 (MQ=255) aaCCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt < 2:353975/139‑1 (MQ=255) aaCCCTCGAAGGGCAGGCGCGTAACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt < 2:419637/139‑1 (MQ=255) cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgc < 1:1696638/54‑1 (MQ=255) cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgc > 2:1696638/1‑54 (MQ=255) cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc < 1:1579045/139‑1 (MQ=255) gCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTg < 2:1234229/130‑1 (MQ=255) gCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATGTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTg > 1:1234229/1‑130 (MQ=255) aggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGc < 2:1222834/56‑1 (MQ=255) aggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGc > 1:1222834/1‑56 (MQ=255) catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc < 2:1380612/56‑1 (MQ=255) catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc < 2:1279687/56‑1 (MQ=255) catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc > 1:1380612/1‑56 (MQ=255) catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc > 1:1279687/1‑56 (MQ=255) | GGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTG > NC_000913/47652‑47884 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |