Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,059	C→T	*186* (TAG→TAA)	yaiS ←	putative PIG‑L family deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,059	0	C	T	100.0%	134.6 / NA	42	*186* (TAG→TAA)	yaiS	putative PIG‑L family deacetylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

TTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGG  >  NC_000913/383931‑384191                                                                                                                                 |                                                                                                                                     ttCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTAt                                                                                                                            >  2:635902/1‑139 (MQ=255)   cATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGt                                                                                                                          <  2:255289/139‑1 (MQ=255)           ttGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTaa                                                                                                                  <  1:635902/139‑1 (MQ=255)               tCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                              <  1:286278/139‑1 (MQ=255)                                      cAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                       <  2:536779/139‑1 (MQ=255)                                              tCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGa                                                                                                    >  2:1535629/1‑118 (MQ=255)                                              tCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGa                                                                                                    <  1:1535629/118‑1 (MQ=255)                                                     gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                            <  1:1470907/135‑1 (MQ=255)                                                     gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                            >  2:1470907/1‑135 (MQ=255)                                                         gCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCaaaa                                                                    >  2:889421/1‑139 (MQ=255)                                                             ttGACCTGTTGTCCGCTAAATTGTGCAACGGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTTTTTAACGGATTCAAAAGCCt                                                                <  1:1178261/139‑1 (MQ=255)                                                                aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTCTTaa                                                                                                                  >  1:708532/1‑86 (MQ=255)                                                                aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTCTTaa                                                                                                                  <  2:708532/86‑1 (MQ=255)                                                                     ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                              <  1:1293521/85‑1 (MQ=255)                                                                     ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                              >  2:1293521/1‑85 (MQ=255)                                                                     ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAata                                                                                          <  1:577997/105‑1 (MQ=255)                                                                     ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAata                                                                                          >  2:577997/1‑105 (MQ=255)                                                                     ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                       >  1:1443217/1‑108 (MQ=255)                                                                     ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                       <  2:1443217/108‑1 (MQ=255)                                                                     ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATa                                                        >  2:71153/1‑139 (MQ=255)                                                                          cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATg                                                    >  1:846658/1‑138 (MQ=255)                                                                          cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATg                                                    >  1:248273/1‑138 (MQ=255)                                                                          cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATg                                                    <  2:846658/138‑1 (MQ=255)                                                                          cgcgAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATg                                                    <  2:248273/138‑1 (MQ=255)                                                                               aaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgcagc                                                                                                            <  2:586221/77‑1 (MQ=255)                                                                               aaTTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgcagc                                                                                                            >  1:586221/1‑77 (MQ=255)                                                                                              cGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGa                                                                                                    >  2:1517634/1‑70 (MQ=255)                                                                                              cGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGa                                                                                                    <  1:1517634/70‑1 (MQ=255)                                                                                                cAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCg                             <  1:71153/139‑1 (MQ=255)                                                                                                           gACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTACTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAAt                  <  1:889421/139‑1 (MQ=255)                                                                                                             cGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAAttt                >  1:1193124/1‑139 (MQ=255)                                                                                                                atatAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                              >  2:1106547/1‑42 (MQ=255)                                                                                                                atatAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                              <  1:1106547/42‑1 (MQ=255)                                                                                                                 tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTaa                                                                                                                  >  1:1174254/1‑37 (MQ=255)                                                                                                                 tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTaa                                                                                                                  <  2:1174254/37‑1 (MQ=255)                                                                                                                 tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTACTGCAGCAAGTTGGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCgg            >  2:1064382/1‑139 (MQ=255)                                                                                                                 tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCTAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCgg            >  2:709117/1‑139 (MQ=255)                                                                                                                 tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCgt            >  2:368687/1‑138 (MQ=255)                                                                                                                 tataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCgg            >  1:1404455/1‑139 (MQ=255)                                                                                                                  ataATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGa           >  1:927660/1‑139 (MQ=255)                                                                                                                         cgtcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTc    <  1:709117/139‑1 (MQ=255)                                                                                                                           tcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCgg  >  1:164890/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                     TTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGG  >  NC_000913/383931‑384191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑