Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	437,107	G→A	*173* (TAG→TAA)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	437,107	0	G	A	100.0%	186.8 / NA	57	*173* (TAG→TAA)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/30);  total (27/30)

CGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCA  >  NC_000913/436971‑437240                                                                                                                                         |                                                                                                                                      cGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAAtt                                                                                                                                     >  1:81055/1‑139 (MQ=255)    ggCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgtg                                                                                                                                  >  1:1358389/1‑139 (MQ=255)                        cGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACg                                                                                                              <  1:523413/139‑1 (MQ=255)                           aaTGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGa                                                                                                                            >  2:235399/1‑122 (MQ=255)                           aaTGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGa                                                                                                                            <  1:235399/122‑1 (MQ=255)                           aaTGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCtt                                                                                                           >  1:104720/1‑139 (MQ=255)                                gCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCAc                                                                                                      <  2:104720/139‑1 (MQ=255)                                       ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               <  1:674152/139‑1 (MQ=255)                                            ggggATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGc                                                                                          <  2:1358389/139‑1 (MQ=255)                                             gggATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGcc                                                                                         <  1:859748/139‑1 (MQ=255)                                                                ttGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATcc                                                                      <  2:1107256/139‑1 (MQ=255)                                                                 tGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               >  1:1051253/1‑113 (MQ=255)                                                                 tGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               <  2:1051253/113‑1 (MQ=255)                                                                     gCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACg                                                                                                     >  2:955515/1‑103 (MQ=255)                                                                     gCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACg                                                                                                     <  1:955515/103‑1 (MQ=255)                                                                               tttCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCgg                                                       <  2:1105906/139‑1 (MQ=255)                                                                                          aTCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATc                                                                       >  2:900259/1‑112 (MQ=255)                                                                                          aTCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATc                                                                       <  1:900259/112‑1 (MQ=255)                                                                                             cTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACg                                                                                                     >  2:804933/1‑79 (MQ=255)                                                                                             cTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACg                                                                                                     <  1:804933/79‑1 (MQ=255)                                                                                                tATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGc                                                                                          >  2:199150/1‑87 (MQ=255)                                                                                                tATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGc                                                                                          <  1:199150/87‑1 (MQ=255)                                                                                                    ttATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                    <  2:818328/73‑1 (MQ=255)                                                                                                    ttATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                    >  1:818328/1‑73 (MQ=255)                                                                                                     tATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCg                                 >  1:375572/1‑139 (MQ=255)                                                                                                     tATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCg                                 <  2:81055/139‑1 (MQ=255)                                                                                                     tATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGACCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTc                                        <  1:436427/132‑1 (MQ=255)                                                                                                     tATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGACCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTc                                        >  2:436427/1‑132 (MQ=255)                                                                                                      aTCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCgg                                >  1:283489/1‑139 (MQ=255)                                                                                                       tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCAcc                                                                                                                              <  1:614015/44‑1 (MQ=255)                                                                                                       tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCAcc                                                                                                                              >  2:614015/1‑44 (MQ=255)                                                                                                       tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                           <  1:1503096/95‑1 (MQ=255)                                                                                                       tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                           >  2:1503096/1‑95 (MQ=255)                                                                                                          gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGa                                                            <  1:1441649/107‑1 (MQ=255)                                                                                                          gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGa                                                            >  2:1441649/1‑107 (MQ=255)                                                                                                          gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATAc                                                <  2:56366/119‑1 (MQ=255)                                                                                                          gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATAc                                                >  1:56366/1‑119 (MQ=255)                                                                                                            catcaCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGAt                                                           >  1:1457270/1‑106 (MQ=255)                                                                                                            catcaCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGAt                                                           <  2:1457270/106‑1 (MQ=255)                                                                                                                aCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGt                                                                                                         <  1:772430/56‑1 (MQ=255)                                                                                                                aCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGt                                                                                                         >  2:772430/1‑56 (MQ=255)                                                                                                                     ccGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCt                 >  2:639773/1‑139 (MQ=255)                                                                                                                       gCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTc                      <  1:1351621/132‑1 (MQ=255)                                                                                                                       gCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTc                      >  2:1351621/1‑132 (MQ=255)                                                                                                                         tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                    >  2:452950/1‑52 (MQ=255)                                                                                                                         tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                    >  2:185856/1‑52 (MQ=255)                                                                                                                         tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                    <  1:452950/52‑1 (MQ=255)                                                                                                                         tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                    <  1:185856/52‑1 (MQ=255)                                                                                                                         tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                                                                                   >  2:679749/1‑69 (MQ=255)                                                                                                                         tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                                                                                   <  1:679749/69‑1 (MQ=255)                                                                                                                                    tCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCa  <  2:375572/139‑1 (MQ=255)                                                                                                                                     cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               >  1:1253790/1‑45 (MQ=255)                                                                                                                                     cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               <  2:1253790/45‑1 (MQ=255)                                                                                                                                     cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                           <  2:1179559/65‑1 (MQ=255)                                                                                                                                     cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                           >  1:1179559/1‑65 (MQ=255)                                                                                                                                     cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGAt                                                           <  2:690717/81‑1 (MQ=255)                                                                                                                                     cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGAt                                                           >  1:690717/1‑81 (MQ=255)                                                                                                                                         |                                                                                                                                      CGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCA  >  NC_000913/436971‑437240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑