Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,604	C→T	S13N (AGT→AAT)  *304* (TAG→TAA)	yajL ← panE ←	oxidative‑stress‑resistance chaperone 2‑dehydropantoate reductase, NADPH‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,604	0	C	T	100.0%	201.6 / NA	62	S13N (AGT→AAT)  *304* (TAG→TAA)	yajL panE	oxidative‑stress‑resistance chaperone 2‑dehydropantoate reductase, NADPH‑specific
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/33);  total (29/33)

CTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAT  >  NC_000913/443486‑443734                                                                                                                       |                                                                                                                                   cTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAAcc                                                                                                                >  2:658046/1‑139 (MQ=255)          gcgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGAt                                                                                                       <  2:683800/139‑1 (MQ=255)                         cGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCAct                                                                                        <  2:64239/139‑1 (MQ=255)                              ggTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCct                                                                                   >  2:418996/1‑139 (MQ=255)                               gTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                      >  2:557193/1‑119 (MQ=255)                               gTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                      <  1:557193/119‑1 (MQ=255)                                     aTCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCtttt                                                                            <  1:951494/139‑1 (MQ=255)                                         cTGGCGACGCTGGCAGTGGTGCCTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                                                                                  <  1:1034799/113‑1 (MQ=255)                                         cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                      >  2:150662/1‑109 (MQ=255)                                         cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                      <  1:150662/109‑1 (MQ=255)                                         cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCt                                                                                                  >  2:1034799/1‑113 (MQ=255)                                                     gCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAgg                                                            <  2:1296413/139‑1 (MQ=255)                                                         tggtgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                                                           <  1:1563295/136‑1 (MQ=255)                                                         tggtgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                                                           >  2:1563295/1‑136 (MQ=255)                                                           gtgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGgtg                                                      >  2:524738/1‑139 (MQ=255)                                                           gtgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGgtg                                                      >  1:890563/1‑139 (MQ=255)                                                            tgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCa                                                                                                                    <  2:1515683/76‑1 (MQ=255)                                                            tgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCa                                                                                                                    >  1:1515683/1‑76 (MQ=255)                                                                    aTACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGt                                             >  1:7333/1‑139 (MQ=255)                                                                     tACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTc                                                                                           <  1:1471893/92‑1 (MQ=255)                                                                     tACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTc                                                                                           >  2:1471893/1‑92 (MQ=255)                                                                         gccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGc                                        <  1:418996/139‑1 (MQ=255)                                                                         gccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGc                                        <  2:781782/139‑1 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                                           >  1:1584050/1‑55 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAgggg                                                                                                                           <  2:1584050/55‑1 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCa                                                                                          <  2:63433/88‑1 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCa                                                                                          >  1:63433/1‑88 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTa                                                                          <  2:1630904/104‑1 (MQ=255)                                                                          ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTa                                                                          >  1:1630904/1‑104 (MQ=255)                                                                                          cGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                      >  1:1163030/1‑60 (MQ=255)                                                                                          cGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                                                                      <  2:1163030/60‑1 (MQ=255)                                                                                            aTAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg                     <  2:7333/139‑1 (MQ=255)                                                                                            aTAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg                     <  1:658046/139‑1 (MQ=255)                                                                                               gtggtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcgcgg                  <  2:890563/139‑1 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAgg                                                                                                                      <  2:429590/36‑1 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAgg                                                                                                                      >  1:429590/1‑36 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTa                                                                          <  1:1004442/80‑1 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                                                           <  2:440080/95‑1 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                                                           >  1:440080/1‑95 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGACAATCCCATGCGCGCGGGCGCGGCGt               >  1:149253/1‑139 (MQ=255)                                                                                                  gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCCCATTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt               >  1:1320527/1‑139 (MQ=255)                                                                                                  gtaaCGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTa                                                                          >  2:1004442/4‑80 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGAt                                                                                                       >  1:448263/1‑46 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGAt                                                                                                       <  2:448263/46‑1 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCaaa                                                                >  1:588291/1‑85 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCaaa                                                                <  2:588291/85‑1 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGttt                                                   >  2:1609630/1‑98 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGttt                                                   <  1:1609630/98‑1 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGttt                                                   <  2:80193/98‑1 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGttt                                                   >  1:80193/1‑98 (MQ=255)                                                                                                       ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAagag          >  1:1325643/1‑139 (MQ=255)                                                                                                        gCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAgaga         <  1:524738/139‑1 (MQ=255)                                                                                                         cTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGACAATCCCATGCGCGCGGGCGCGGCGTAAGAGaa        <  2:149253/139‑1 (MQ=255)                                                                                                          ttCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGGGCTCATATTCa                                                                                          >  1:1294365/1‑56 (MQ=255)                                                                                                          ttCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCa                                                                                          <  2:1294365/56‑1 (MQ=255)                                                                                                            cAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTa                                                                          <  1:1379636/70‑1 (MQ=255)                                                                                                            cAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTa                                                                          >  2:1379636/1‑70 (MQ=255)                                                                                                               tctcTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAt  <  2:1325643/139‑1 (MQ=255)                                                                                                               tctcTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTC‑CTCTCCTTTCTTTTTACCATTTCAAACAGGCGGgtg                                                      <  2:1530004/86‑1 (MQ=255)                                                                                                               tctcTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTC‑CTCTCCTTTCTTTTTACCATTTCAAACAGGCGGgtg                                                      >  1:1530004/1‑86 (MQ=255)                                                                                                                   ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAgg                                                            >  1:1314940/1‑77 (MQ=255)                                                                                                                   ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAgg                                                            <  2:1314940/77‑1 (MQ=255)                                                                                                                       |                                                                                                                                   CTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAT  >  NC_000913/443486‑443734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑