Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	475,667	T→C	S97P (TCA→CCA)	ybaY →	outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	475,667	0	T	C	100.0%	133.2 / NA	37	S97P (TCA→CCA)	ybaY	outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/20);  total (17/20)

GTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCC  >  NC_000913/475533‑475788                                                                                                                                       |                                                                                                                          gTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCAcc                                                                                                                       >  2:1712117/1‑139 (MQ=255)              ccGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCa                                                                                                                         >  1:679501/1‑124 (MQ=255)               cGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTc                                                                                                         <  1:652625/139‑1 (MQ=255)                                    ccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                         <  2:824412/134‑1 (MQ=255)                                    ccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                         >  1:824412/1‑134 (MQ=255)                                              tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa                                                                          <  2:128615/139‑1 (MQ=255)                                                   aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                     <  2:719540/139‑1 (MQ=255)                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCACAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                     >  1:1050509/1‑134 (MQ=255)                                                        gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCACAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                     <  2:1050509/134‑1 (MQ=255)                                                             tttCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGtt                                                           <  2:1655709/139‑1 (MQ=255)                                                                           ttAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTa                                             <  1:566561/139‑1 (MQ=255)                                                                                   tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCt                                                                                                        <  1:60794/72‑1 (MQ=255)                                                                                   tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCt                                                                                                        >  2:60794/1‑72 (MQ=255)                                                                                   tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTa                                                                  <  1:423540/110‑1 (MQ=255)                                                                                   tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTa                                                                  >  2:423540/1‑110 (MQ=255)                                                                                   tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTa                                             >  2:734211/1‑131 (MQ=255)                                                                                   tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTa                                             <  1:734211/131‑1 (MQ=255)                                                                                             aaaGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                    >  1:784047/1‑50 (MQ=255)                                                                                             aaaGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                    <  2:784047/50‑1 (MQ=255)                                                                                               aGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                     <  2:719973/95‑1 (MQ=255)                                                                                               aGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                     >  1:719973/1‑95 (MQ=255)                                                                                                          aGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGa              <  2:1501714/139‑1 (MQ=255)                                                                                                           gAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                    <  2:1511641/36‑1 (MQ=255)                                                                                                           gAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                    >  1:1511641/1‑36 (MQ=38)                                                                                                            aaaGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                     >  2:1283143/1‑82 (MQ=255)                                                                                                            aaaGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                     <  1:1283143/82‑1 (MQ=255)                                                                                                                   tGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCa     >  2:554476/1‑139 (MQ=255)                                                                                                                    gCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCt                                                                                                        >  1:1060549/1‑39 (MQ=255)                                                                                                                    gCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCt                                                                                                        <  2:1060549/39‑1 (MQ=255)                                                                                                                     cGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGc   >  2:946499/1‑139 (MQ=255)                                                                                                                      gTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                         >  2:575673/1‑52 (MQ=255)                                                                                                                      gTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                         <  1:575673/52‑1 (MQ=255)                                                                                                                      gTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGcc  <  1:1218452/139‑1 (MQ=255)                                                                                                                             aGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaa                                <  1:805197/102‑1 (MQ=255)                                                                                                                             aGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaa                                >  2:805197/1‑102 (MQ=255)                                                                                                                                 aaaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGtt                                                           <  2:251082/71‑1 (MQ=255)                                                                                                                                 aaaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGtt                                                           >  1:251082/1‑71 (MQ=255)                                                                                                                                       |                                                                                                                          GTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCC  >  NC_000913/475533‑475788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑