Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,763	2 bp→TC	coding (2017‑2018/2145 nt)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,763	0	C	T	100.0%	127.2 / NA	40	L673S (CTG→TCG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/19);  total (21/19)
*	NC_000913	2,416,764	0	T	C	100.0%	142.2 / NA	40	L673S (CTG→TCG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

TGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAA  >  NC_000913/2416645‑2416891                                                                                                                       ||                                                                                                                                tGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtg                                                                                                              <  2:406787/139‑1 (MQ=255)    cGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctg                                                                                                           >  2:887732/1‑139 (MQ=255)        gCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAgg                                                                                                       >  2:605432/1‑139 (MQ=255)                   aGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGT‑TGCGCAAGc                                                                                           <  1:1087000/139‑1 (MQ=255)                        cGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCg                                                                                                                 >  2:520686/1‑113 (MQ=255)                        cGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCg                                                                                                                 <  1:520686/113‑1 (MQ=255)                        cGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGt                                                                                       >  1:1147156/1‑139 (MQ=255)                                  ggTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctgc                                                                                                          <  2:487654/110‑1 (MQ=255)                                  ggTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctgc                                                                                                          >  1:487654/1‑110 (MQ=255)                                      gCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCTCCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGATGCAGGGTATGCGCAAGc                                                                                           >  1:1090007/1‑121 (MQ=255)                                      gCAGGTCGCGCTACCGTGTTCAACTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGc                                                                                           <  2:1090007/121‑1 (MQ=255)                                       cAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTACAGGGTATGCGCAAGCCGGTTAACGACCTGCCCCg                                                                        <  2:791429/139‑1 (MQ=255)                                         ggTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTg                                                                      <  1:1467361/139‑1 (MQ=255)                                                           aTCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                    <  2:1147156/139‑1 (MQ=255)                                                           aTCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                    <  1:887732/139‑1 (MQ=255)                                                                         gAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATc                                      >  1:970113/1‑139 (MQ=255)                                                                          aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGAtc                                                                                                                             >  2:1731998/1‑51 (MQ=38)                                                                          aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCaa                                                                                             <  1:354068/83‑1 (MQ=255)                                                                          aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCaa                                                                                             >  2:354068/1‑83 (MQ=255)                                                                          aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                    >  1:559389/1‑124 (MQ=255)                                                                          aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                    <  2:559389/124‑1 (MQ=255)                                                                          aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATcg                                     >  1:546397/1‑139 (MQ=255)                                                                          aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATcg                                     >  2:594729/1‑139 (MQ=255)                                                                              ccGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCt                                 <  1:594729/139‑1 (MQ=255)                                                                              ccGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCt                                 <  2:359641/139‑1 (MQ=255)                                                                               cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCa                                                                                              <  1:810434/77‑1 (MQ=255)                                                                               cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCa                                                                                              >  2:810434/1‑77 (MQ=255)                                                                               cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTg                                >  1:1096187/1‑139 (MQ=255)                                                                                 gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctgc                                                                                                          <  1:565092/63‑1 (MQ=255)                                                                                 gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAtgctgc                                                                                                          >  2:565092/1‑63 (MQ=255)                                                                                 gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGat                                                     >  2:260656/1‑116 (MQ=255)                                                                                 gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGat                                                     <  1:260656/116‑1 (MQ=255)                                                                                 gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGAc                              >  2:70366/1‑139 (MQ=255)                                                                                 gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGAc                              >  1:527643/1‑139 (MQ=255)                                                                                         accTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATcgcg                                   <  2:1119704/126‑1 (MQ=255)                                                                                         accTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTATACACCATcgcg                                   >  1:1119704/1‑126 (MQ=255)                                                                                                      tACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCAcagca         >  1:634142/1‑139 (MQ=255)                                                                                                           cGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGt                                                                       >  1:30701/1‑72 (MQ=255)                                                                                                           cGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGt                                                                       <  2:30701/72‑1 (MQ=255)                                                                                                             ttCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTaa  <  2:634142/139‑1 (MQ=255)                                                                                                                       ||                                                                                                                                TGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAA  >  NC_000913/2416645‑2416891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑