Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 582,148 Δ1 bp intergenic (+51/+4) tfaD → / ← ybcY pseudogene, DLP12 prophage, tail fiber assembly protein family,Phage or Prophage Related/pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913582,1470G.96.7% 246.0 / 1.2 61intergenic (+50/+5)tfaD/ybcYpseudogene, DLP12 prophage, tail fiber assembly protein family,Phage or Prophage Related/pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (24/35);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

TGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTT  >  NC_000913/582011‑582280
                                                                                                                                        |                                                                                                                                     
tGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgat                                                                                                                                     >  1:252798/1‑137 (MQ=255)
  gAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCACAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                                   >  2:449354/1‑135 (MQ=255)
                    gCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                                <  2:529893/139‑1 (MQ=255)
                       gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             <  1:532785/139‑1 (MQ=255)
                       gAACCGTGTTGATACATCAACTGCAACTGATATTGAGTGGCCTACGAACCCTGTTAGGGAATAATCAATGGGATTATGCCGCAGCACGTCTTAAGCAAGAACCTGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             <  1:943160/139‑1 (MQ=255)
                       gAACCGTGCTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             <  1:626615/139‑1 (MQ=255)
                            gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        <  1:1024096/139‑1 (MQ=255)
                             tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        >  1:262420/1‑139 (MQ=255)
                             tgtTGATACATCAACTGCACCTGACATCGAGTGGCCTACGAACCCTGTCTGGGAGTAATCATTGGGCTTATGCCGCAGCATGCCTTAAGCAAGCACATGCAGCGGTCGATGCTATTTTTTTCCTGATTCGGAAAACAtt                                                                                                       >  1:168241/1‑139 (MQ=255)
                              gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                      <  2:151904/139‑1 (MQ=255)
                               ttGATACATCAACTGCACCTCATATTGAGTGTCCTACGAACCCTGTCAGCAAGTAATCATTGGGATTATGCCGCACCACGTCTTAAGCAAGAACATGCTCCGCTTGATCTTATTTTTTTCCTGAAGCGGAAAACATtac                                                                                                     <  2:168241/139‑1 (MQ=255)
                                 gATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                   <  1:721481/139‑1 (MQ=255)
                                   tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                 <  2:824736/139‑1 (MQ=255)
                                    aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAATAACATGCTTCTGTTGATGTTAGATTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                <  2:1033403/139‑1 (MQ=255)
                                    aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                 <  2:262420/139‑1 (MQ=255)
                                       tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTcc                                                                                                                       >  1:1022915/1‑113 (MQ=255)
                                       tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTcc                                                                                                                       <  2:1022915/113‑1 (MQ=255)
                                       tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                             >  1:910529/1‑139 (MQ=255)
                                           cTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTg                                                                                         >  1:703151/1‑139 (MQ=255)
                                               aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                     <  1:265046/139‑1 (MQ=255)
                                                ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             >  1:653337/1‑114 (MQ=255)
                                                ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             <  2:653337/114‑1 (MQ=255)
                                                 cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGTGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        <  1:778739/118‑1 (MQ=255)
                                                 cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        >  2:778739/1‑118 (MQ=255)
                                                        tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                                   <  1:574823/85‑5 (MQ=255)
                                                        tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                                   >  2:574823/1‑81 (MQ=255)
                                                        tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                            >  2:804345/1‑139 (MQ=255)
                                                           gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACa                                                                         >  2:114053/1‑139 (MQ=255)
                                                            tGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                        >  1:56470/1‑139 (MQ=255)
                                                             ggCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAtt                                                                       >  2:321412/1‑139 (MQ=255)
                                                              gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                      >  1:725737/1‑139 (MQ=255)
                                                              gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                      >  2:504658/1‑139 (MQ=255)
                                                                  aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                  <  2:725737/139‑1 (MQ=255)
                                                                   cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCACAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                 <  1:449354/139‑1 (MQ=255)
                                                                    gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                <  1:114053/139‑1 (MQ=255)
                                                                        ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                            <  2:973292/139‑1 (MQ=255)
                                                                         cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             >  1:235749/1‑89 (MQ=255)
                                                                         cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             <  2:235749/89‑1 (MQ=255)
                                                                         cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        <  1:404970/94‑1 (MQ=255)
                                                                         cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        >  2:404970/1‑94 (MQ=255)
                                                                          tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          <  1:216831/139‑1 (MQ=255)
                                                                          tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          <  2:56470/139‑1 (MQ=255)
                                                                           gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                                >  1:620362/1‑84 (MQ=255)
                                                                           gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGAAGTTATTTTTTTCCTGAAGCg                                                                                                                <  2:620362/84‑1 (MQ=255)
                                                                            tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                        <  2:910529/139‑1 (MQ=255)
                                                                               gggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGagag                                                     <  2:252798/139‑1 (MQ=255)
                                                                                      aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                <  1:600629/121‑1 (MQ=255)
                                                                                      aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                >  2:600629/1‑121 (MQ=255)
                                                                                         aTTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGtt                                           <  1:504658/139‑1 (MQ=255)
                                                                                                  aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTg                                                                                                                     <  1:350182/56‑1 (MQ=255)
                                                                                                  aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTg                                                                                                                     >  2:350182/1‑56 (MQ=255)
                                                                                                  aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                        >  1:411811/1‑117 (MQ=255)
                                                                                                  aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                        <  2:411811/117‑1 (MQ=255)
                                                                                                       gcagcaCGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGa                             <  1:917088/139‑1 (MQ=255)
                                                                                                             cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAAc                                                                                                          <  1:154814/56‑1 (MQ=255)
                                                                                                             cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAAc                                                                                                          >  2:154814/1‑56 (MQ=255)
                                                                                                                         gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                           >  1:856975/1‑43 (MQ=255)
                                                                                                                         gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                           <  2:856975/43‑1 (MQ=37)
                                                                                                                          aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                           <  1:1031125/74‑1 (MQ=255)
                                                                                                                          aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                           >  2:1031125/1‑74 (MQ=255)
                                                                                                                              tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                            >  1:821460/1‑85 (MQ=255)
                                                                                                                              tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                            <  2:821460/85‑1 (MQ=255)
                                                                                                                              tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc      >  2:67172/1‑139 (MQ=255)
                                                                                                                              tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc      >  2:47668/1‑139 (MQ=255)
                                                                                                                               gctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGcc     >  2:869053/1‑139 (MQ=255)
                                                                                                                                  gcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt  <  1:804345/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTT  >  NC_000913/582011‑582280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: