Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,763,987 | Δ1 bp | coding (23/1488 nt) | sufB ← | component of SufBCD Fe‑S cluster assembly scaffold |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,763,987 | 0 | G | . | 98.2% | 227.6 / ‑2.9 | 56 | coding (23/1488 nt) | sufB | component of SufBCD Fe‑S cluster assembly scaffold |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (26/29); minor base A (0/1); total (26/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTG > NC_000913/1763854‑1764120 | cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt < 1:1025784/139‑1 (MQ=255) cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt < 2:60610/139‑1 (MQ=255) cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt < 2:510023/139‑1 (MQ=255) cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCtt < 2:381888/139‑1 (MQ=255) cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCAAGGTTTTGACATCGTCA‑TTGCtt > 2:543011/1‑139 (MQ=255) cGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACgag > 2:953642/1‑139 (MQ=255) cGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACa < 2:82794/139‑1 (MQ=255) cgcgcACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCg > 1:252314/1‑139 (MQ=255) accaccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAg > 2:996503/1‑114 (MQ=255) accaccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAg < 1:996503/114‑1 (MQ=255) tttATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCtt < 2:583817/139‑1 (MQ=255) tATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTc < 2:99136/139‑1 (MQ=255) cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgcca < 1:11636/139‑1 (MQ=255) cTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccaca > 2:843917/1‑139 (MQ=255) tttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑‑TGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgc > 2:986207/1‑133 (MQ=255) tttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑‑TGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgc < 1:986207/133‑1 (MQ=255) ttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc < 2:638093/139‑1 (MQ=255) ttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc > 1:249817/1‑139 (MQ=255) ttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc > 2:674528/1‑139 (MQ=255) aGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCAcat < 2:607166/139‑1 (MQ=255) cTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACTTAGTACCGCTTATACCCCAAA‑GCTTTCCCCACAGCCAcattc > 1:1033096/1‑139 (MQ=255) aTCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCAcattcatt > 1:457226/1‑139 (MQ=255) cGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCCGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCt < 2:633164/139‑1 (MQ=255) ggTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTg < 1:843917/139‑1 (MQ=255) ggTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTg < 2:252314/139‑1 (MQ=255) cgttggtAAATGGGTGAAGGATTCCTTTTTTTTAATTCAGCGGGCGGCCGTTCCAGTTTTTGCCACCG‑CAATTCTTTAAGTTTTACAGAACATATTACCGCTTATACCCCAAA‑GCTTTCGCAACGGCACCATCCATTCt < 2:821919/133‑1 (MQ=255) gTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGGCTTTCGCCACAGCCACATTCATTCTg < 2:604566/139‑1 (MQ=255) gCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgcca < 2:92837/118‑1 (MQ=255) gCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgcca > 1:92837/1‑118 (MQ=255) gCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCt > 2:384183/1‑139 (MQ=255) ggTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt > 1:595402/1‑139 (MQ=255) a‑gaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGaa < 2:206136/139‑1 (MQ=255) cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg < 1:620664/63‑1 (MQ=255) cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg > 2:620664/1‑63 (MQ=255) tcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat < 1:42827/139‑1 (MQ=255) ttttTAATTCCGCCGGCCGCCCGTCCAAGGTTTTACATCCTCA‑TTTCTTCAGTATTACCAGACCTAGTACCCCTTATACCCCCAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGGTGTGAAATTTGAatat < 1:543011/139‑1 (MQ=255) tttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTAGGCTTTAGGGTTGTGAAATTTGAatat < 1:674528/139‑1 (MQ=255) ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat > 1:1001100/1‑136 (MQ=255) ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat < 2:1001100/136‑1 (MQ=255) tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa > 2:803040/1‑133 (MQ=255) tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa < 1:803040/133‑1 (MQ=255) ataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCt > 1:328815/1‑108 (MQ=255) ataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCt < 2:328815/108‑1 (MQ=255) cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg > 1:291764/1‑139 (MQ=255) cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg < 2:756328/139‑1 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg > 2:884981/1‑47 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACg < 1:884981/47‑1 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAggg < 2:337168/110‑1 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAggg > 1:337168/1‑110 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa > 1:168222/1‑123 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa < 2:168222/123‑1 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc > 1:353045/1‑139 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc > 1:838803/1‑139 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc > 1:856333/1‑139 (MQ=255) cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc > 2:129615/1‑139 (MQ=255) gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTc > 2:142854/1‑139 (MQ=255) tCGTCA‑TTGATTCAGTATTACGATACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGCCTTCCGTg > 2:924011/1‑139 (MQ=255) | CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTG > NC_000913/1763854‑1764120 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |