Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,763,987 Δ1 bp coding (23/1488 nt) sufB ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,9870G.98.2% 227.6 / ‑2.9 56coding (23/1488 nt)sufBcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (26/29);  minor base A (0/1);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTG  >  NC_000913/1763854‑1764120
                                                                                                                                      |                                                                                                                                      
cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCtt                                                                                                                                  <  1:1025784/139‑1 (MQ=255)
cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCtt                                                                                                                                  <  2:60610/139‑1 (MQ=255)
cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCtt                                                                                                                                  <  2:510023/139‑1 (MQ=255)
cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCtt                                                                                                                                  <  2:381888/139‑1 (MQ=255)
cTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCAAGGTTTTGACATCGTCATTGCtt                                                                                                                                  >  2:543011/1‑139 (MQ=255)
            cGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACgag                                                                                                                      >  2:953642/1‑139 (MQ=255)
               cGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACa                                                                                                                   <  2:82794/139‑1 (MQ=255)
                       cgcgcACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCg                                                                                                           >  1:252314/1‑139 (MQ=255)
                            accaccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAg                                                                                                                               >  2:996503/1‑114 (MQ=255)
                            accaccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAg                                                                                                                               <  1:996503/114‑1 (MQ=255)
                                        tttATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCtt                                                                                         <  2:583817/139‑1 (MQ=255)
                                          tATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTc                                                                                       <  2:99136/139‑1 (MQ=255)
                                              cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                                   <  1:11636/139‑1 (MQ=255)
                                                cTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccaca                                                                                 >  2:843917/1‑139 (MQ=255)
                                                 tttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑‑TGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                     >  2:986207/1‑133 (MQ=255)
                                                 tttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑‑TGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                     <  1:986207/133‑1 (MQ=255)
                                                  ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                               <  2:638093/139‑1 (MQ=255)
                                                  ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                               >  1:249817/1‑139 (MQ=255)
                                                  ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                               >  2:674528/1‑139 (MQ=255)
                                                       aGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                          <  2:607166/139‑1 (MQ=255)
                                                         cTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACTTAGTACCGCTTATACCCCAAAGCTTTCCCCACAGCCAcattc                                                                        >  1:1033096/1‑139 (MQ=255)
                                                            aTCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcattcatt                                                                     >  1:457226/1‑139 (MQ=255)
                                                              cGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCCGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                   <  2:633164/139‑1 (MQ=255)
                                                               ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                  <  1:843917/139‑1 (MQ=255)
                                                               ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                  <  2:252314/139‑1 (MQ=255)
                                                               cgttggtAAATGGGTGAAGGATTCCTTTTTTTTAATTCAGCGGGCGGCCGTTCCAGTTTTTGCCACCGCAATTCTTTAAGTTTTACAGAACATATTACCGCTTATACCCCAAAGCTTTCGCAACGGCACCATCCATTCt                                                                   <  2:821919/133‑1 (MQ=255)
                                                                gTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGGCTTTCGCCACAGCCACATTCATTCTg                                                                  <  2:604566/139‑1 (MQ=255)
                                                                   gCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                                   <  2:92837/118‑1 (MQ=255)
                                                                   gCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                                   >  1:92837/1‑118 (MQ=255)
                                                                   gCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCt                                                              >  2:384183/1‑139 (MQ=255)
                                                                           ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt                                                      >  1:595402/1‑139 (MQ=255)
                                                                                agaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGaa                                                 <  2:206136/139‑1 (MQ=255)
                                                                                       cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                        <  1:620664/63‑1 (MQ=255)
                                                                                       cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                        >  2:620664/1‑63 (MQ=255)
                                                                                         tcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                         <  1:42827/139‑1 (MQ=255)
                                                                                           ttttTAATTCCGCCGGCCGCCCGTCCAAGGTTTTACATCCTCATTTCTTCAGTATTACCAGACCTAGTACCCCTTATACCCCCAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGGTGTGAAATTTGAatat                                       <  1:543011/139‑1 (MQ=255)
                                                                                           tttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTAGGCTTTAGGGTTGTGAAATTTGAatat                                       <  1:674528/139‑1 (MQ=255)
                                                                                            ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                         >  1:1001100/1‑136 (MQ=255)
                                                                                            ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                         <  2:1001100/136‑1 (MQ=255)
                                                                                             tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                           >  2:803040/1‑133 (MQ=255)
                                                                                             tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                           <  1:803040/133‑1 (MQ=255)
                                                                                              ataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                   >  1:328815/1‑108 (MQ=255)
                                                                                              ataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                   <  2:328815/108‑1 (MQ=255)
                                                                                                    cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg                              >  1:291764/1‑139 (MQ=255)
                                                                                                    cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg                              <  2:756328/139‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                        >  2:884981/1‑47 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                                        <  1:884981/47‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAggg                                                        <  2:337168/110‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAggg                                                        >  1:337168/1‑110 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                           >  1:168222/1‑123 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                           <  2:168222/123‑1 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                           >  1:353045/1‑139 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                           >  1:838803/1‑139 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                           >  1:856333/1‑139 (MQ=255)
                                                                                                       cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                           >  2:129615/1‑139 (MQ=255)
                                                                                                                            gACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTc      >  2:142854/1‑139 (MQ=255)
                                                                                                                                tCGTCATTGATTCAGTATTACGATACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGCCTTCCGTg  >  2:924011/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CTCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTG  >  NC_000913/1763854‑1764120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: