Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 247,466 Δ1 bp intergenic (+5/‑171) yafL → / → rayT putative lipoprotein and C40 family peptidase/RAYT REP element‑mobilizing transposase, TnpA(REP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913247,4650G.100.0% 144.7 / NA 36intergenic (+4/‑172)yafL/rayTputative lipoprotein and C40 family peptidase/RAYT REP element‑mobilizing transposase, TnpA(REP)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/23);  total (13/23)

TATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247327‑247594
                                                                                                                                          |                                                                                                                                 
tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACg                                                                                                                                   >  1:150671/1‑139 (MQ=255)
          aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGtt                                                                                                                        >  1:1047995/1‑139 (MQ=255)
                     aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATc                                                                                                             <  1:719017/139‑1 (MQ=255)
                           tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                       <  2:891894/139‑1 (MQ=255)
                           tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                       <  2:30194/139‑1 (MQ=255)
                            cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  2:376932/135‑1 (MQ=255)
                            cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                                          >  2:815386/1‑135 (MQ=255)
                            cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                                          >  1:376932/1‑135 (MQ=255)
                            cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:815386/135‑1 (MQ=255)
                                     ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                             <  1:689783/139‑1 (MQ=255)
                                          gAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                        <  1:862716/139‑1 (MQ=255)
                                               cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                   <  1:610905/139‑1 (MQ=255)
                                                  tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                >  1:982234/1‑139 (MQ=255)
                                                        aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                          <  1:221103/139‑1 (MQ=255)
                                                         aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:7012/139‑1 (MQ=255)
                                                         aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:322716/139‑1 (MQ=255)
                                                                 aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                 <  2:982234/139‑1 (MQ=255)
                                                                    cGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                              <  2:150671/139‑1 (MQ=255)
                                                                           ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                       >  1:721236/1‑139 (MQ=255)
                                                                             cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                     <  1:661099/139‑1 (MQ=255)
                                                                                 cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                 <  1:988272/139‑1 (MQ=255)
                                                                                     aCCATATTTTGGGCGCGCGCAGGTTTTTGACGAAAGGGACGATTTTGACAGACGATAAGACGTTTACGCCACATCCAGCAGTTGTACGCTGGTGCCTGATGCGAGGCTGGCGCGGATGATGAGGACGACGAACCCGCGa                                             >  2:101921/1‑139 (MQ=16)
                                                                                                 gcgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGc                                 >  2:624892/1‑139 (MQ=255)
                                                                                                   gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAGGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                   <  1:258700/87‑1 (MQ=37)
                                                                                                   gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCg                               <  2:627336/139‑1 (MQ=255)
                                                                                                   gcgcgcAGGATTTTGACGGAAGAGACGAATTTGTAAGACGATAAGGCGTTTACGCCGCATCAGGCAGTTGTACGCAGGTGCCTGATg                                                                                   >  2:258700/1‑87 (MQ=25)
                                                                                                         aGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:1007462/139‑1 (MQ=255)
                                                                                                         aGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:76252/139‑1 (MQ=255)
                                                                                                              tttGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGata                    <  1:1029470/139‑1 (MQ=255)
                                                                                                                       agagACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaac           >  2:307601/1‑139 (MQ=255)
                                                                                                                         agaCGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt         <  1:307601/139‑1 (MQ=255)
                                                                                                                           aCGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:1016924/83‑1 (MQ=255)
                                                                                                                           aCGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:736851/83‑1 (MQ=255)
                                                                                                                           aCGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               >  1:1016924/1‑83 (MQ=255)
                                                                                                                           aCGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               >  1:736851/1‑83 (MQ=18)
                                                                                                                              aTTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTg    >  1:979503/1‑139 (MQ=255)
                                                                                                                                tttGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  >  1:215964/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                 
TATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247327‑247594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: