Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,238,562 (T)7→6 intergenic (+265/‑18) ygjR → / → alx putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,238,5560T.98.5% 269.5 / ‑2.5 65intergenic (+259/‑24)ygjR/alxputative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (29/35);  minor base G (1/0);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCAT  >  NC_000913/3238418‑3238686
                                                                                                                                          |                                                                                                                                  
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                    >  1:729480/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                    >  2:450586/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                    >  2:913654/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                    >  2:571123/1‑139 (MQ=255)
  tgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttt                                                                                                                                  >  1:687927/1‑139 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                               <  1:698814/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                               <  2:432173/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                               <  1:450586/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                               <  1:260171/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                               <  1:313486/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                               <  1:41205/139‑1 (MQ=255)
       aaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                             <  1:1008412/139‑3 (MQ=255)
          cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTAtgtg                                                                                                                         >  2:707571/1‑139 (MQ=255)
            caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                       >  2:173610/1‑139 (MQ=255)
                 ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                  <  2:836658/139‑1 (MQ=255)
                     aCCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTc                                                                                                              <  1:571123/139‑1 (MQ=255)
                       cGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTa                                                                                                            <  1:639440/139‑1 (MQ=255)
                         ggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATg                                                                                                          >  2:880704/1‑139 (MQ=255)
                                      gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGTGAGGTCTATGCATAAAAAGCAGCTGCTGACGTCTTCCGATGTTGGCCGTTCTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                             <  1:671194/139‑1 (MQ=255)
                                      gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                             <  2:578892/139‑1 (MQ=255)
                                        aTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACg                                                                                           >  1:800913/1‑139 (MQ=255)
                                           cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                             >  1:335962/1‑134 (MQ=255)
                                           cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                             <  2:335962/134‑1 (MQ=255)
                                              aGACGTATTCCTTTTTTGTTGTAAGTGAGTCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTg                                                                                     <  2:681276/139‑1 (MQ=255)
                                               gACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGc                                                                                    >  2:283166/1‑139 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                                   >  2:924209/1‑139 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                                   >  1:64593/1‑139 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                                   >  2:942979/1‑139 (MQ=255)
                                                 cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCt                                                                                                             >  2:426286/1‑112 (MQ=255)
                                                 cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCt                                                                                                             <  1:426286/112‑1 (MQ=255)
                                                 cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa                                                                                  <  1:173610/139‑1 (MQ=255)
                                                     ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATggg                                                                              >  2:925922/1‑139 (MQ=255)
                                                     ttCCTTTTTTGATGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTGCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATggg                                                                              >  2:26291/1‑139 (MQ=255)
                                                         ttttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCgg                                                                          <  2:100775/139‑1 (MQ=255)
                                                          tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                         >  2:161552/1‑139 (MQ=255)
                                                            tttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCGGTTGCTATGGGGCGGAtt                                                                       >  1:255766/1‑139 (MQ=255)
                                                              tgttgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCg                                                                     <  1:925922/139‑1 (MQ=255)
                                                                 tgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtg                                                                  <  2:687927/139‑1 (MQ=255)
                                                                  gtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgt                                                                 >  2:338333/1‑139 (MQ=255)
                                                                  gtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgt                                                                 >  1:499463/1‑139 (MQ=255)
                                                                     aGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgt                                                              >  2:883103/1‑139 (MQ=255)
                                                                      gTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgtt                                                             <  1:913654/139‑1 (MQ=255)
                                                                          gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTc                                                                      >  1:232476/1‑126 (MQ=255)
                                                                          gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTc                                                                      <  2:232476/126‑1 (MQ=255)
                                                                             cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                      <  2:800913/139‑1 (MQ=255)
                                                                             cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                      <  1:338333/139‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCGGTTTTTTATGTGTa                                                                                                                       <  2:450619/72‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                  >  2:884291/1‑77 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                  <  1:884291/77‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCGGTTTTTTATGTGTa                                                                                                                       >  1:450619/1‑72 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                    <  1:75065/59‑1 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                    >  2:75065/1‑59 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTa                                                   >  1:897921/1‑139 (MQ=255)
                                                                                  cGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATg                                                 <  1:988027/139‑1 (MQ=255)
                                                                                         cGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTa                                          <  1:707571/139‑1 (MQ=255)
                                                                                             gTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                                      <  1:942979/139‑1 (MQ=255)
                                                                                              tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                     <  2:897921/139‑1 (MQ=255)
                                                                                              tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                     <  2:161350/139‑1 (MQ=255)
                                                                                                  tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                             >  1:19687/1‑79 (MQ=255)
                                                                                                  tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                             <  2:19687/79‑1 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                      >  1:412038/1‑113 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                      <  2:412038/113‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                  >  2:390246/1‑52 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                  <  1:390246/52‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                     <  2:713523/129‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                     >  1:713523/1‑129 (MQ=255)
                                                                                                             cagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                                      <  1:726181/123‑1 (MQ=255)
                                                                                                             cagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                                      >  2:726181/1‑123 (MQ=255)
                                                                                                             cagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAgggg                      <  2:499463/139‑1 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGc                     >  1:556457/1‑139 (MQ=255)
                                                                                                                 ggCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                >  2:235139/1‑45 (MQ=255)
                                                                                                                 ggCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                <  1:235139/45‑1 (MQ=38)
                                                                                                                    tGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTg               <  2:500667/139‑1 (MQ=255)
                                                                                                                      aCGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTggg             >  1:287475/1‑139 (MQ=255)
                                                                                                                       cGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTgggg            <  1:795332/139‑1 (MQ=255)
                                                                                                                        gTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGc           <  1:883103/139‑1 (MQ=255)
                                                                                                                         tCTTTCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAGTACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca          <  2:255766/139‑1 (MQ=255)
                                                                                                                          cTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcac         <  1:161552/139‑1 (MQ=255)
                                                                                                                                aCGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                          <  2:571732/52‑1 (MQ=255)
                                                                                                                                aCGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                          >  1:571732/1‑52 (MQ=255)
                                                                                                                                 cGTTGGCCGTTTTTTATTTGTATGGATCTTCTATGAATATTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat  <  2:729480/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                  
TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCAT  >  NC_000913/3238418‑3238686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: