Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,759,680 (C)5→6 coding (844/1221 nt) sufS ← cysteine desulfurase, stimulated by SufE, selenocysteine lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,759,6751.C100.0% 166.0 / NA 47G283G (GGC→GGGsufScysteine desulfurase, stimulated by SufE, selenocysteine lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/23);  total (24/23)

GGGCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCC  >  NC_000913/1759541‑1759792
                                                                                                                                       |                                                                                                                     
gggCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGcccc                                                                                                                   <  1:747768/139‑1 (MQ=255)
gggCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGcccc                                                                                                                   <  1:644379/139‑1 (MQ=255)
 ggCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGccccc                                                                                                                  <  1:367862/139‑1 (MQ=255)
    cATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGccccccgg                                                                                                               >  2:74565/1‑136 (MQ=255)
        gagagTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTAtt                                                                                                            <  2:880390/139‑1 (MQ=255)
        gagagTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTAtt                                                                                                            <  2:956878/139‑1 (MQ=255)
                     ggTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCt                                                                                               <  2:140999/139‑1 (MQ=255)
                     ggTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCt                                                                                               >  1:810218/1‑139 (MQ=255)
                      gTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCtt                                                                                              <  1:107881/139‑1 (MQ=255)
                       tACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTc                                                                                             >  2:300766/1‑139 (MQ=255)
                           gATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAAc                                                                                         <  2:810218/139‑1 (MQ=255)
                            aTTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAAcc                                                                                        >  1:746533/1‑139 (MQ=255)
                              tCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGc                                                                                      >  2:830335/1‑139 (MQ=255)
                                      tgATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGc                                                                              >  1:86287/1‑139 (MQ=255)
                                       gATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                             <  1:29500/139‑1 (MQ=255)
                                       gATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                             <  2:356866/139‑1 (MQ=255)
                                       gATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                             <  1:123998/139‑1 (MQ=255)
                                             gcATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTc                                                                       >  1:566062/1‑139 (MQ=255)
                                                    gCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAg                                                                <  2:989421/139‑1 (MQ=255)
                                                        cAGATTCTGTTCATCCTCGACTATGTTATTAAGCCCCAGCGCCGAAACATACTCTAGCACCGCGCCAAGACCAATGATGCCCCCCGGCATTGGGTGTACCGACTTCAAACCGCCATGGCGCTTTTGTCAAGGTAGTGcc                                                            >  2:364537/1‑139 (MQ=255)
                                                             tCTGTTCATACTCGGTTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTCTTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCAc                                                       >  1:562320/1‑139 (MQ=255)
                                                                 ttCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAg                                                   >  1:602018/1‑139 (MQ=255)
                                                                  tCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAgg                                                  >  1:165681/1‑139 (MQ=255)
                                                                    aTACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCt                                                >  2:911486/1‑139 (MQ=255)
                                                                     tACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTg                                               >  1:419770/1‑139 (MQ=255)
                                                                      aCTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGa                                              >  1:679643/1‑139 (MQ=255)
                                                                      aCTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGa                                              >  1:148095/1‑139 (MQ=255)
                                                                        tCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACg                                            <  2:165681/139‑1 (MQ=255)
                                                                           gCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTg                                         >  1:919339/1‑139 (MQ=255)
                                                                             tATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                       <  1:300766/139‑1 (MQ=255)
                                                                             tATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                       <  2:746533/139‑1 (MQ=255)
                                                                             tATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                       <  2:679643/139‑1 (MQ=255)
                                                                                         ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                       <  1:841836/127‑1 (MQ=255)
                                                                                         ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                       >  2:841836/1‑127 (MQ=255)
                                                                                         ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGaa                             >  2:456159/1‑137 (MQ=255)
                                                                                         ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGaa                             <  1:456159/137‑1 (MQ=255)
                                                                                         ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAAcc                           >  1:513068/1‑139 (MQ=255)
                                                                                         ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACCGTGGCGATCATAGAAcc                           >  1:917235/1‑139 (MQ=255)
                                                                                          cccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                             <  1:211283/88‑1 (MQ=255)
                                                                                          cccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                             >  2:211283/1‑88 (MQ=255)
                                                                                           ccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGc                         >  1:913118/1‑139 (MQ=255)
                                                                                           ccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGACTGACGGTGGCGATCATAGAACCGc                         <  2:562320/139‑1 (MQ=255)
                                                                                            cAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGcc                        >  2:953998/1‑139 (MQ=255)
                                                                                                  cGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGt                                                                  >  1:988789/1‑91 (MQ=255)
                                                                                                  cGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGt                                                                  <  2:988789/91‑1 (MQ=255)
                                                                                                            tCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACggcggc        <  2:148095/139‑1 (MQ=255)
                                                                                                            tCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACggcggc        <  2:651922/139‑1 (MQ=255)
                                                                                                            tCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACggcggc        <  2:602018/139‑1 (MQ=255)
                                                                                                                  gccgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTcc  <  2:1041336/139‑1 (MQ=255)
                                                                                                                      cgccAAGACCAATGATGCCCCCCGGTATTGGGTGTAc                                                                                                    >  2:286260/1‑37 (MQ=255)
                                                                                                                      cgccAAGACCAATGATGCCCCCCGGTATTGGGTGTAc                                                                                                    <  1:286260/37‑1 (MQ=255)
                                                                                                                                       |                                                                                                                     
GGGCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCC  >  NC_000913/1759541‑1759792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: