Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,850	(C)6→7	coding (260/552 nt)	btuE ←	glutathione peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,844	1	.	C	100.0%	127.2 / NA	36	V89G (GTG→GGG)	btuE	glutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/19);  total (17/19)

GGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑CCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAA  >  NC_000913/1793707‑1793974                                                                                                                                           |                                                                                                                                   ggCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑c                                                                                                                                   >  1:793638/1‑139 (MQ=255)  gCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑cc                                                                                                                                  <  1:76585/139‑1 (MQ=255)        gCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑ccccccca                                                                                                                            <  2:793638/139‑3 (MQ=255)        gCTGACCAGACGGGCACAGAACCCGCTCTCTCCCGGCGCGACTGGGGTCGGCGCTGCGGCAATCAAGTTTTGATACCGCAGATGGCGTCCTTCGCCACTAACTTCAATCATACTGAACATCGGGAACGTCA‑ccccccca                                                                                                                            <  2:385696/139‑3 (MQ=255)          tGACCATACAGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑cccccccatg                                                                                                                          <  1:675409/139‑5 (MQ=255)             ccATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATgtgg                                                                                                                        <  1:514697/139‑1 (MQ=255)                 aCGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATgtggt                                                                                                                       <  1:202129/136‑1 (MQ=255)                 aCGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATgtggt                                                                                                                       >  2:202129/1‑136 (MQ=255)                 aCGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTggtggt                                                                                                                    >  2:355537/1‑139 (MQ=255)                  cGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTa                                                                                                                   <  1:256012/139‑1 (MQ=255)                           aaTCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGtt                                                                                                          >  2:181718/1‑139 (MQ=255)                                gCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                     <  2:351348/139‑1 (MQ=255)                                gCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                     <  1:1010292/139‑1 (MQ=255)                                 ctctctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGtggtgg                                                                                                                     >  2:858379/1‑122 (MQ=255)                                 ctctctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGtggtgg                                                                                                                     <  1:858379/122‑1 (MQ=255)                                 ctctctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAtc                                                                                                    >  1:383629/1‑139 (MQ=255)                                     ctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCtt                                                                                                >  1:279260/1‑139 (MQ=255)                                                aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATctct                                                                                                 >  1:434781/1‑127 (MQ=255)                                                aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATctct                                                                                                 <  2:434781/127‑1 (MQ=255)                                                                cGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGaa                                                                     >  2:699157/1‑139 (MQ=255)                                                                          tttttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGCACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCa                                                           >  2:751107/1‑139 (MQ=255)                                                                              tGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACggg                                                       <  1:653022/139‑1 (MQ=255)                                                                              tGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACggg                                                       <  1:355537/139‑1 (MQ=255)                                                                              tGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACggg                                                       <  2:279260/139‑1 (MQ=255)                                                                                        gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCa                                             >  1:766110/1‑139 (MQ=255)                                                                                        gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCa                                             <  2:766110/139‑1 (MQ=255)                                                                                        gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCa                                             <  2:383629/139‑1 (MQ=255)                                                                                                ccTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa                                     >  2:456776/1‑139 (MQ=255)                                                                                                     gCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcg                                >  2:445561/1‑139 (MQ=255)                                                                                                      ccATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcga                               <  1:991563/139‑1 (MQ=255)                                                                                                       cATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                              <  1:1013948/139‑1 (MQ=255)                                                                                                          tAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGtt                                                                                                          <  2:27112/60‑1 (MQ=255)                                                                                                          tAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGtt                                                                                                          >  1:27112/1‑60 (MQ=255)                                                                                                                        cTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAAt                                                    >  2:135969/1‑100 (MQ=255)                                                                                                                        cTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAAt                                                    <  1:135969/100‑1 (MQ=255)                                                                                                                                cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                              <  1:570603/114‑1 (MQ=255)                                                                                                                                cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                              >  2:570603/1‑114 (MQ=255)                                                                                                                                cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtctc     >  2:379381/1‑139 (MQ=255)                                                                                                                                cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtctc     >  2:347242/1‑139 (MQ=255)                                                                                                                                 gggAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc    <  1:379381/139‑1 (MQ=255)                                                                                                                                   gAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa  <  1:262095/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                   GGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑CCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAA  >  NC_000913/1793707‑1793974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑