Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,728,118 (C)8→9 coding (1254/1455 nt) xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,728,1101.C95.6% 230.1 / 2.1 68coding (1262/1455 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (23/42);  total (24/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA  >  NC_000913/3727977‑3728236
                                                                                                                                        |                                                                                                                                
aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccc                                                                                                                            <  2:250557/139‑1 (MQ=255)
aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccc                                                                                                                            <  2:952374/139‑1 (MQ=255)
   aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGTGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑cccccccc                                                                                                                         <  1:33020/139‑1 (MQ=255)
        cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑cccccccccgtac                                                                                                                    <  1:187676/139‑6 (MQ=255)
        cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑CCCCCCCCGTACg                                                                                                                    >  2:779348/1‑139 (MQ=255)
             cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTaa                                                                                                                <  1:658164/139‑1 (MQ=255)
               cTGCGCATCTGGTAGTTGCGACTGTTCTAACGGTAGTTGCGGCAACCATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGTCCCAGC‑CA‑‑CCCCCCCCGTACgggactc                                                                                                              <  2:248740/139‑6 (MQ=255)
                  cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccccccc                                                                                                                        <  1:61034/125‑2 (MQ=255)
                  cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccccccc                                                                                                                        >  2:61034/1‑124 (MQ=255)
                      tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAgc‑tgc                                                                                                      >  1:28097/1‑139 (MQ=255)
                      tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAgc‑tgc                                                                                                      >  1:423541/1‑139 (MQ=255)
                       cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAgc‑tgct                                                                                                     <  1:769943/139‑1 (MQ=255)
                       cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGCACGGTAATCGAgc‑tgct                                                                                                     >  2:547851/1‑139 (MQ=255)
                         ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGa                                                                                                   <  2:201014/139‑1 (MQ=255)
                         ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGa                                                                                                   <  2:982765/139‑1 (MQ=255)
                          gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGAc                                                                                                  <  2:856665/139‑1 (MQ=255)
                          gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGAc                                                                                                  <  2:859429/139‑1 (MQ=255)
                          gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGAc                                                                                                  <  1:565250/139‑1 (MQ=255)
                              aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCt                                                                                              >  1:460975/1‑139 (MQ=255)
                                 cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGat                                                                                           >  1:428567/1‑139 (MQ=255)
                                 cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGat                                                                                           <  2:84152/139‑1 (MQ=255)
                                       ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGc                                                                                     >  1:317434/1‑139 (MQ=255)
                                        tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCAAGC‑TGCTGACCGCGGCGATCCGcc                                                                                    >  2:238788/1‑139 (MQ=255)
                                          tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAg                                                                                  <  1:362058/139‑1 (MQ=255)
                                               gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCt                                                                             >  1:471425/1‑139 (MQ=255)
                                               gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCt                                                                             <  1:89552/139‑1 (MQ=255)
                                               gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCt                                                                             <  1:922260/139‑1 (MQ=255)
                                               gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccc                                                                                                                            >  2:320789/1‑92 (MQ=255)
                                               gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccc                                                                                                                            <  1:320789/92‑1 (MQ=255)
                                                 aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           >  2:44636/1‑139 (MQ=255)
                                                 aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           <  2:859840/139‑1 (MQ=255)
                                                 aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCCCGC‑TGCTGACCGCTGATATCCCCCAACATCTGa                                                                           >  2:121174/1‑139 (MQ=255)
                                                 aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccccc                                                                                                                          <  2:121292/92‑1 (MQ=255)
                                                 aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccccc                                                                                                                          >  1:121292/1‑92 (MQ=255)
                                                   ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                         <  2:471425/139‑1 (MQ=255)
                                                   ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                         >  1:934672/1‑139 (MQ=255)
                                                     gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                       <  2:68165/139‑1 (MQ=255)
                                                      cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                      >  2:126883/1‑139 (MQ=255)
                                                      cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                      <  2:57426/139‑1 (MQ=255)
                                                                tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                            <  1:423519/139‑1 (MQ=255)
                                                                      aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGAAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                      >  2:942031/1‑139 (MQ=255)
                                                                       gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccccc                                                                                                                          >  1:892300/1‑70 (MQ=255)
                                                                       gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑ccccccc                                                                                                                          <  2:892300/70‑1 (MQ=255)
                                                                       gCGATTTCTCTGGATTCGCCGCAATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                     <  1:172050/139‑1 (MQ=255)
                                                                        cGATTTCTCTGGATTCGCCGCGTTCTGCGCCAGCCCTGCTGCGCCCAGATCTGGCCCCA‑C‑ATCCCCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCATCATCTGACGCCAGTACTCACTACGcgccc                                                     <  2:984012/139‑1 (MQ=255)
                                                                         gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                         >  1:103835/1‑117 (MQ=255)
                                                                         gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                         <  2:103835/117‑1 (MQ=255)
                                                                               tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑A‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                             <  1:44636/139‑1 (MQ=255)
                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑CAAC‑‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           <  1:703453/139‑1 (MQ=255)
                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           <  1:126883/139‑1 (MQ=255)
                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           <  2:460975/139‑1 (MQ=255)
                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           <  2:934672/139‑1 (MQ=255)
                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           <  1:760476/139‑1 (MQ=255)
                                                                                      tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                      <  1:942031/139‑1 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATCCCCCCCCCCCTACGGTAATC‑AGCGTGCTGACCGCTGTGATCCGCCAGCATTTGACGCCAGTACTCACCACGCGCCACACCCCCAATCaccct                                     >  1:921164/1‑135 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                    >  1:886642/1‑139 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           >  2:980343/1‑132 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                           <  1:980343/132‑1 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGtc                                    >  1:338264/1‑138 (MQ=255)
                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                            <  2:428567/139‑1 (MQ=255)
                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                            <  1:381697/139‑1 (MQ=255)
                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                            <  2:306361/139‑1 (MQ=255)
                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                           <  2:423541/139‑1 (MQ=255)
                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                           <  2:28097/139‑1 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTaa                                                                                                                >  1:820077/1‑47 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTaa                                                                                                                <  2:820077/47‑1 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                  >  2:1046471/1‑92 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                  <  1:1046471/92‑1 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑cccccccccgtt                                                                                                                     >  2:775522/1‑37 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑cccccccccgta                                                                                                                     <  1:775522/41‑5 (MQ=255)
                                                                                                           ttGCTGCGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                 >  1:720995/1‑139 (MQ=255)
                                                                                                             gctgcGCCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg               <  2:720995/139‑1 (MQ=255)
                                                                                                                gcgcCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg            <  1:694030/139‑1 (MQ=255)
                                                                                                                  gcCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc          <  1:859218/139‑1 (MQ=255)
                                                                                                                  gcCCAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTACGGTTTAATACCGCAGGc          <  2:584958/139‑1 (MQ=255)
                                                                                                                   cccAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGa                                                                                                   <  1:879384/49‑1 (MQ=255)
                                                                                                                   cccAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGa                                                                                                   >  2:879384/1‑49 (MQ=255)
                                                                                                                   cccAGTGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa         <  1:786561/139‑1 (MQ=255)
                                                                                                                        tGCTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc    <  1:515676/139‑1 (MQ=255)
                                                                                                                          cTGGCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa  <  1:169823/139‑1 (MQ=255)
                                                                                                                             gCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                    <  1:538335/118‑1 (MQ=255)
                                                                                                                             gCCCCA‑C‑ATC‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                    >  2:538335/1‑118 (MQ=255)
                                                                                                                                        |                                                                                                                                
ATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCA‑C‑AT‑‑CCCCCCCCGTACGGTAATCGAGC‑TGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA  >  NC_000913/3727977‑3728236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑