Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,965,418 (GA)4→5 coding (213/1266 nt) rhlB ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,965,4101.G95.7% 75.2 / ‑3.2 23coding (221/1266 nt)rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (14/8);  total (15/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
*NC_0009133,965,4102.A95.7% 68.3 / ‑3.3 23coding (221/1266 nt)rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (14/8);  total (15/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑GAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCG  >  NC_000913/3965273‑3965533
                                                                                                                                          ||                                                                                                                             
cGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑g                                                                                                                              <  2:408453/139‑1 (MQ=255)
       cAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑gagaga‑‑ga                                                                                                                     <  1:136846/139‑1 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc                                                         >  1:1053896/1‑139 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc                                                         >  1:787758/1‑139 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc                                                         >  2:17697/1‑139 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑gagaga‑‑gag                                                                                                                    >  1:139076/1‑77 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑gagaga‑‑gag                                                                                                                    <  2:139076/78‑2 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑gagaga‑‑g                                                                                                                      <  2:635597/76‑1 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑gagaga‑‑g                                                                                                                      >  1:635597/1‑76 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑gag                                                                                                                            <  1:368362/72‑1 (MQ=255)
                                                                     cAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑gag                                                                                                                            >  2:368362/1‑72 (MQ=255)
                                                                               cgTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGt                                               >  2:383739/1‑139 (MQ=255)
                                                                                                  gCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATTGTTTTCCCGGTACCGGTTTGCGCCTGcc                                                        <  1:123132/111‑1 (MQ=255)
                                                                                                  gCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATTGTTTTCCCGGTACCGGTTTGCGCCTGcc                                                        >  2:123132/1‑111 (MQ=255)
                                                                                                   cACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTACCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCACCAGCGTCCGc                           <  2:612616/139‑1 (MQ=255)
                                                                                                    aCGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCg                          >  2:540084/1‑139 (MQ=255)
                                                                                                     cgcgGCTGATTCACCTTGCGATCGGCAATCGCAGGGT‑‑GAGAGAGCGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCCGCTACGTCACGACCCGCCAGCGTCCGCgg                         >  2:491/1‑139 (MQ=255)
                                                                                                     cgcgGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCgg                         >  1:38735/1‑139 (MQ=255)
                                                                                                     cgcgGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCgg                         >  2:465292/1‑139 (MQ=255)
                                                                                                      gcgGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGa                        <  1:465292/139‑1 (MQ=255)
                                                                                                       cgGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGaa                       >  2:669796/1‑139 (MQ=255)
                                                                                                       cgGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGaa                       >  1:585150/1‑139 (MQ=255)
                                                                                                        ggCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCCGCGTCAGCGGTAg                      >  2:699335/1‑139 (MQ=255)
                                                                                                           tGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGc                   <  2:86064/139‑1 (MQ=255)
                                                                                                           tGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGc                   <  2:1053896/139‑1 (MQ=255)
                                                                                                                       cGATCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAAt       >  1:604171/1‑139 (MQ=255)
                                                                                                                         aTCGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATgg     >  1:86753/1‑139 (MQ=255)
                                                                                                                           cGGCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGc   >  2:657057/1‑139 (MQ=255)
                                                                                                                            ggCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCg  <  2:38735/139‑1 (MQ=255)
                                                                                                                            ggCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCg  <  2:277157/139‑1 (MQ=255)
                                                                                                                            ggCAATCGCAGGATGAGAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCg  <  2:765640/139‑1 (MQ=255)
                                                                                                                                          ||                                                                                                                             
CGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGAT‑‑GAGAGA‑‑GAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCG  >  NC_000913/3965273‑3965533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑