Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,222,6410GA50.0% 2.0 / 59.5 46G153S (GGC→AGC) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base G (13/10);  new base A (15/8);  total (28/18)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGC  >  NC_000913/2222505‑2222779
                                                                                                                                        |                                                                                                                                          
gCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATcagc                                                                                                                                          <  1:476276/139‑1 (MQ=255)
 cACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATcagcg                                                                                                                                         <  1:519601/139‑1 (MQ=255)
        ttGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTg                                                                                                                                  >  1:344138/1‑139 (MQ=255)
          ggCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTa                                                                                                                                <  2:475313/139‑1 (MQ=255)
                ggCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACt                                                                                                                          >  2:353715/1‑139 (MQ=255)
                ggCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACt                                                                                                                          >  2:531496/1‑139 (MQ=255)
                ggCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACt                                                                                                                          >  1:818185/1‑139 (MQ=255)
                  cGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATcggc                                                                                                                                          >  1:9021/1‑121 (MQ=255)
                  cGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATcggc                                                                                                                                          <  2:9021/121‑1 (MQ=255)
                    aaCAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCcgg                                                                                                                      >  1:101076/1‑139 (MQ=255)
                         gTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCt                                                                                                                 <  2:969903/139‑1 (MQ=255)
                          tGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTc                                                                                                                >  2:459910/1‑139 (MQ=255)
                           gCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCg                                                                                                               <  2:422465/139‑1 (MQ=255)
                            cTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGc                                                                                                              <  1:353715/139‑1 (MQ=255)
                              ggCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCt                                                                                                                 <  1:194112/134‑1 (MQ=255)
                              ggCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCt                                                                                                                 >  2:194112/1‑134 (MQ=255)
                                         gCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCt                                                                                                                 >  1:901380/1‑123 (MQ=255)
                                         gCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCt                                                                                                                 <  2:901380/123‑1 (MQ=255)
                                           accacGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGcc                                                                                               <  1:531496/139‑1 (MQ=255)
                                                gCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCg                                                                                          <  2:667145/139‑1 (MQ=255)
                                                         gCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGaa                                                                                 <  1:836429/139‑1 (MQ=255)
                                                          cTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGaaa                                                                                >  2:1043180/1‑139 (MQ=255)
                                                           tGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAAt                                                                               >  2:66508/1‑139 (MQ=255)
                                                            ggAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATg                                                                              >  1:616883/1‑139 (MQ=255)
                                                                       tCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGc                                                                                                <  1:248390/110‑1 (MQ=255)
                                                                       tCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGc                                                                                                >  2:248390/1‑110 (MQ=255)
                                                                       tCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTCTTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTcgc                                                                   <  2:616883/139‑1 (MQ=255)
                                                                        cAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTcgcg                                                                  <  1:262522/139‑1 (MQ=255)
                                                                          aaCCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTcgcgcg                                                                <  1:66508/139‑1 (MQ=255)
                                                                                              cACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAAc                                            >  1:63358/1‑139 (MQ=255)
                                                                                                cTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTg                                          >  2:840636/1‑139 (MQ=255)
                                                                                                           gATCATCGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGaa                               >  2:469492/1‑139 (MQ=255)
                                                                                                               atcGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCAt                           >  2:212995/1‑139 (MQ=255)
                                                                                                               atcGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCAt                           >  1:891679/1‑139 (MQ=255)
                                                                                                               atcGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGc                                                                                                >  1:939992/1‑70 (MQ=255)
                                                                                                               atcGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGc                                                                                                <  2:939992/70‑1 (MQ=255)
                                                                                                               atcGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAAc                              >  2:353710/1‑136 (MQ=255)
                                                                                                               atcGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAAc                              <  1:353710/136‑1 (MQ=255)
                                                                                                                 cGTGTATAGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCt                         >  1:980390/1‑139 (MQ=255)
                                                                                                                        aGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGc                                      <  1:211324/119‑1 (MQ=255)
                                                                                                                        aGGCGCATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGc                                      >  2:211324/1‑119 (MQ=255)
                                                                                                                          gcgcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGta                                                                                        >  2:15782/1‑67 (MQ=255)
                                                                                                                          gcgcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGta                                                                                        <  1:15782/67‑1 (MQ=255)
                                                                                                                          gcgcATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCg                                                                                                               <  2:496762/44‑1 (MQ=255)
                                                                                                                          gcgcATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCg                                                                                                               >  1:496762/1‑44 (MQ=255)
                                                                                                                          gcgcATCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGa                >  2:573177/1‑139 (MQ=255)
                                                                                                                            gcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATc              >  1:1033972/1‑139 (MQ=255)
                                                                                                                             cATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCt             >  2:759491/1‑139 (MQ=255)
                                                                                                                             cATCGGTCATAGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTCTTCGCGCGTATAAATGAAGACGGCCAACTGACGCTGGTGAACCATCTGGGGATTGATCt             >  2:662579/1‑139 (MQ=255)
                                                                                                                               tCGGTCATCAGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTgg           >  2:481306/1‑139 (MQ=255)
                                                                                                                                        ggcggTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGc  <  2:184845/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGC  >  NC_000913/2222505‑2222779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑