Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	499,014	C→T	R320R (CGC→CGT)  *320* (TAG→TAA)	hemH → aes ←	ferrochelatase acetyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,014	0	C	T	100.0%	91.3 / NA	29	R320R (CGC→CGT)  *320* (TAG→TAA)	hemH aes	ferrochelatase acetyl esterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)

CTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGC  >  NC_000913/498883‑499145                                                                                                                                    |                                                                                                                                    cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                              >  1:251606/1‑139 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                              >  2:922631/1‑139 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                              >  2:815163/1‑139 (MQ=255) cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCt                                                                                                                              <  2:113804/139‑1 (MQ=255) cTGGAAGAGACTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCAGGCGGGAAAAAATATTTATATATGTCGGCGCTTAATGCCACGCGGGAACATATCGAAATGATGGCTAACCTTGTTGCAGCCTATCGTTaacggc                                                                                                                              >  2:481941/1‑135 (MQ=255)    gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                           <  2:93949/139‑1 (MQ=255)    gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAg                                                                                                                           <  2:885440/139‑1 (MQ=255)           ttGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTaaa                                                                                                                    <  1:589675/139‑1 (MQ=255)              ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGaa                                                                                                                 >  1:27463/1‑139 (MQ=255)                   cAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAg                                                                                                            <  1:815163/139‑1 (MQ=255)                                              ggcggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcggcgg                                                                                 >  2:647488/1‑139 (MQ=255)                                               gcggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGt                                                                                >  2:999848/1‑139 (MQ=255)                                                     aaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                          <  2:251606/139‑1 (MQ=255)                                                                  tatTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaataa                                                             >  1:383589/1‑139 (MQ=255)                                                                     tCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAgcg                                                                                                          <  2:504106/91‑1 (MQ=255)                                                                     tCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAgcg                                                                                                          >  1:504106/1‑91 (MQ=255)                                                                     tCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGc                                                          >  2:77813/1‑139 (MQ=255)                                                                              tAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCg                                                 <  2:27463/139‑1 (MQ=255)                                                                                     aCGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCg                                          <  2:817735/139‑1 (MQ=255)                                                                                       gCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcatca                                                                        <  2:492543/107‑1 (MQ=255)                                                                                       gCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcatca                                                                        >  1:492543/1‑107 (MQ=255)                                                                                                    gAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGtt                           <  2:866307/139‑1 (MQ=255)                                                                                                         gatgGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGTGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAAc                      >  2:422317/1‑139 (MQ=255)                                                                                                          atgGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACt                     >  1:1024626/1‑139 (MQ=255)                                                                                                            ggCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGTGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                   <  1:422317/139‑1 (MQ=255)                                                                                                             gCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTcac                  <  2:1024626/139‑1 (MQ=255)                                                                                                                    ttgttgCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGgctg           >  2:569771/1‑139 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGt                                                                   >  1:541176/1‑74 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGt                                                                   <  2:541176/74‑1 (MQ=255)                                                                                                                             cgTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATgcgc  >  1:923786/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                    CTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGC  >  NC_000913/498883‑499145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑