Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	518,721	C→A	M143I (ATG→ATT)	ybbO ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	518,721	0	C	A	100.0%	112.4 / NA	35	M143I (ATG→ATT)	ybbO	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/18);  total (17/18)

AGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATAC  >  NC_000913/518593‑518853                                                                                                                                 |                                                                                                                                     aGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATAc                                                                                                                            <  1:744579/139‑1 (MQ=255)       aCTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCtt                                                                                                                      >  1:714119/1‑139 (MQ=255)             aTTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGt                                                                                                                <  1:150180/139‑1 (MQ=255)                 cGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATcaca                                                                                                                                <  2:13139/119‑1 (MQ=255)                 cGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATcaca                                                                                                                                >  1:13139/1‑119 (MQ=255)                   cTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTa                                                                                                          <  2:441688/139‑1 (MQ=255)                                  aTGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa                                                                                           <  1:591965/139‑1 (MQ=255)                                          tGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATg                                                                                   >  2:836584/1‑139 (MQ=255)                                               cTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAg                                                                              >  2:646694/1‑139 (MQ=255)                                                      cGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgtgt                                                                       >  1:108950/1‑139 (MQ=255)                                                                 cATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGaaaaa                                                            >  1:787064/1‑139 (MQ=255)                                                                            gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCGCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                    <  2:151065/88‑1 (MQ=255)                                                                            gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCGCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATcg                                                                                                    >  1:151065/1‑88 (MQ=255)                                                                              cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCg                                                                                                                 <  1:622808/73‑1 (MQ=255)                                                                              cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCg                                                                                                                 >  2:622808/1‑73 (MQ=255)                                                                              cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAc                                               >  1:253118/1‑139 (MQ=255)                                                                               gcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGgc                                                                                        >  1:117316/1‑97 (MQ=255)                                                                               gcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGgc                                                                                        <  2:117316/97‑1 (MQ=255)                                                                               gcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAct                                              <  1:968806/139‑1 (MQ=255)                                                                               gcgTAAGCGCCACGAACCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAAct                                              <  2:758900/139‑1 (MQ=255)                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                             >  1:187390/1‑139 (MQ=255)                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                             >  1:305432/1‑139 (MQ=255)                                                                                cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                             >  2:42197/1‑139 (MQ=255)                                                                                 gTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActgc                                            <  2:108950/139‑1 (MQ=255)                                                                                                cGGCGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCa                             <  2:648023/139‑1 (MQ=255)                                                                                                   cGTGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACgg                          >  1:554832/1‑139 (MQ=255)                                                                                                     tGGAGATTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCt                        <  2:187390/139‑1 (MQ=255)                                                                                                       gagaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGa                      <  1:238559/139‑1 (MQ=255)                                                                                                       gagaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGa                      >  1:222663/1‑139 (MQ=255)                                                                                                        agaTTAATCCCATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGAt                     >  2:112921/1‑139 (MQ=255)                                                                                                                cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa             <  2:305432/139‑1 (MQ=255)                                                                                                                cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa             <  1:836584/139‑1 (MQ=255)                                                                                                                cccATCACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGaaa             <  2:554832/139‑1 (MQ=255)                                                                                                                     cACCGATGATGTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAggggg        <  2:222663/139‑1 (MQ=255)                                                                                                                           tgatgTAATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATAc  >  1:938957/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                     AGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATAC  >  NC_000913/518593‑518853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑