Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 540,515 C→T intergenic (+7/‑50) allB → / → ybbY allantoinase/putative uracil/xanthine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913540,5150CT100.0% 145.8 / NA 45intergenic (+7/‑50)allB/ybbYallantoinase/putative uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/20);  total (25/20)

TGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCC  >  NC_000913/540388‑540650
                                                                                                                               |                                                                                                                                       
tGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTGCGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                              <  1:57671/139‑1 (MQ=255)
                 cgcgTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGTCCGTCCTTGCGGCGGGCa                                                                                                             >  1:235278/1‑139 (MQ=255)
                      aTCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtctc                                                                                                        <  2:722847/139‑1 (MQ=255)
                              aaCCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAg                                                                                                <  2:515994/139‑1 (MQ=255)
                                           ggtgATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGc                                                                                                                                    <  2:833609/90‑1 (MQ=255)
                                           ggtgATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGc                                                                                                                                    >  1:833609/1‑90 (MQ=255)
                                               atgtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                              >  2:587413/1‑92 (MQ=255)
                                               atgtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGcc                                                                                                                              <  1:587413/92‑1 (MQ=255)
                                                    atTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtgtgt                                                                                          <  2:968349/123‑1 (MQ=255)
                                                    atTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtgtgt                                                                                          >  1:968349/1‑123 (MQ=255)
                                                              ttGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCcgc                                                                >  2:768110/1‑139 (MQ=255)
                                                               tGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGTCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCcgcg                                                               <  2:235278/139‑1 (MQ=255)
                                                               tGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCa                                                                                                             >  1:505215/1‑93 (MQ=255)
                                                               tGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCa                                                                                                             <  2:505215/93‑1 (MQ=255)
                                                                    aGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGc                                                          <  2:964046/139‑1 (MQ=255)
                                                                        cTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGt                                                      >  1:489273/1‑139 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCa                                                                                                             <  1:500385/81‑1 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCa                                                                                                             >  2:500385/1‑81 (MQ=255)
                                                                           cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTAt                                                   >  2:377882/1‑139 (MQ=255)
                                                                                ttGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGa                                              >  1:249612/1‑139 (MQ=255)
                                                                                ttGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGa                                              >  2:136680/1‑139 (MQ=255)
                                                                                ttGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGa                                              >  2:180864/1‑139 (MQ=255)
                                                                                          aGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGt                                    >  2:79859/1‑139 (MQ=255)
                                                                                                      ccTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTAttt                        >  1:349128/1‑139 (MQ=255)
                                                                                                       cTTAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTAtttt                       >  2:121113/1‑139 (MQ=255)
                                                                                                         tAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCt                                                        >  1:1019350/1‑104 (MQ=255)
                                                                                                         tAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTAtttttt                     >  1:1053176/1‑139 (MQ=255)
                                                                                                         tAAACATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTGTGCAGTCAGCCGCGAAAGCCt                                                        <  2:1019350/104‑1 (MQ=255)
                                                                                                           aaCATCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGc                   >  2:934160/1‑139 (MQ=255)
                                                                                                               tCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAaca               >  2:213839/1‑139 (MQ=255)
                                                                                                               tCAGCAGTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAaca               <  2:249612/139‑1 (MQ=255)
                                                                                                                cagcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtc                                                                                                          >  2:1016279/1‑47 (MQ=255)
                                                                                                                cagcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATtc                                                                                                          <  1:1016279/47‑1 (MQ=255)
                                                                                                                cagcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTa                                                                                                  >  2:331692/1‑55 (MQ=255)
                                                                                                                cagcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTa                                                                                                  <  1:331692/55‑1 (MQ=255)
                                                                                                                cagcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac              <  1:768110/139‑1 (MQ=255)
                                                                                                                cagcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac              <  1:180864/139‑1 (MQ=255)
                                                                                                                  gcagTAATCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACgg            >  1:147922/1‑139 (MQ=255)
                                                                                                                       aaTCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCcgcg                                                               <  1:845870/83‑1 (MQ=255)
                                                                                                                       aaTCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCcgcg                                                               >  2:845870/1‑83 (MQ=255)
                                                                                                                        aTCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTgg      >  2:769972/1‑139 (MQ=255)
                                                                                                                         tCTGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGt     <  1:79859/139‑1 (MQ=255)
                                                                                                                           tGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCaa                 >  1:167545/1‑125 (MQ=255)
                                                                                                                           tGGCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCaa                 <  2:167545/125‑1 (MQ=255)
                                                                                                                            ggCTCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTtcc  <  2:349128/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                       
TGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCC  >  NC_000913/540388‑540650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: