Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	594,382	C→T	G130D (GGC→GAC)	cusS ←	copper‑sensing histidine kinase in two‑component regulatory system with CusR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	594,382	0	C	T	100.0%	146.6 / NA	46	G130D (GGC→GAC)	cusS	copper‑sensing histidine kinase in two‑component regulatory system with CusR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/22);  total (24/22)

AATCGATCGAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGCCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACAT  >  NC_000913/594248‑594517                                                                                                                                       |                                                                                                                                        aaTCGATCGAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGa                                                                                                                                     >  2:160649/1‑139 (MQ=255)  atcgatcgAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGaa                                                                                                                                    <  1:67264/139‑1 (MQ=255)      atcgaAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGga                                                                                                                                >  1:102829/1‑139 (MQ=255)                  aTGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGc                                                                                                                    <  1:93935/139‑1 (MQ=255)                             ataAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCg                                                                                                         >  2:872120/1‑139 (MQ=255)                             ataAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCg                                                                                                         >  2:317980/1‑139 (MQ=255)                             ataAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCg                                                                                                         >  1:866798/1‑139 (MQ=255)                                  tCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCttt                                                                                                    >  1:1003813/1‑139 (MQ=255)                                    ggTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTAt                                                                                                  >  2:977946/1‑139 (MQ=255)                                     gTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATc                                                                                                 <  1:977946/139‑1 (MQ=255)                                      tttGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATa                                                                                                                            <  2:964875/111‑1 (MQ=255)                                      tttGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATa                                                                                                                            >  1:964875/1‑111 (MQ=255)                                      tttGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCg                                                                                                >  2:185630/1‑139 (MQ=255)                                                       ggCCAACCGGCAAGTTAATCATCCGCCAGTCGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCt                                                                                                      <  1:1010157/116‑1 (MQ=255)                                                       ggCCAACCGGCAAGTTAATCATCCGCCAGTCGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCt                                                                                                      >  2:1010157/1‑116 (MQ=255)                                                             ccGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTAACGCGTAAACTc                                                                         >  2:630751/1‑139 (MQ=255)                                                                       aaTCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTAt                                                                                                  >  1:839107/1‑104 (MQ=255)                                                                       aaTCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTAt                                                                                                  <  2:839107/104‑1 (MQ=255)                                                                         tcatcCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCgg                                                             <  1:795652/139‑1 (MQ=255)                                                                             ccGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCa                                                         <  1:317980/139‑1 (MQ=255)                                                                             ccGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCa                                                         >  1:308097/1‑139 (MQ=255)                                                                              cGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCAc                                                        <  1:738045/139‑1 (MQ=255)                                                                                 cAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCgg                                                     <  2:102829/139‑1 (MQ=255)                                                                                             tCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATaca                                         <  2:185728/139‑1 (MQ=255)                                                                                                 tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTcg                                                                        <  1:873790/104‑1 (MQ=255)                                                                                                 tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTcg                                                                        >  2:873790/1‑104 (MQ=255)                                                                                                 tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATc                                                                <  2:123583/112‑1 (MQ=255)                                                                                                 tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATc                                                                >  1:123583/1‑112 (MQ=255)                                                                                                       cGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTAcc                               <  1:433129/139‑1 (MQ=255)                                                                                                        gTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTAccc                              <  1:872120/139‑1 (MQ=255)                                                                                                         tGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCt                             >  1:721147/1‑139 (MQ=255)                                                                                                         tGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCt                             >  2:247006/1‑139 (MQ=255)                                                                                                         tGACCGTGGCCTGGCAGCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGCGTGATACACCGTTTTACCCt                             <  1:185630/139‑1 (MQ=255)                                                                                                          gACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTg                            >  1:393362/1‑139 (MQ=255)                                                                                                           aCCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGa                           >  1:51995/1‑139 (MQ=255)                                                                                                             cGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACt                         >  1:700960/1‑139 (MQ=255)                                                                                                                ggCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATc                      <  2:916400/139‑1 (MQ=255)                                                                                                                    tGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGcc                  >  1:564707/1‑139 (MQ=255)                                                                                                                       catcatcatCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAgg               <  2:393362/139‑1 (MQ=255)                                                                                                                           atcatcGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGaaa           >  2:880441/1‑139 (MQ=255)                                                                                                                            tcatcGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAAt          >  1:425541/1‑139 (MQ=255)                                                                                                                              atcGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCa        <  1:160649/139‑1 (MQ=255)                                                                                                                               tcgtcgGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCaa       <  1:360929/139‑1 (MQ=255)                                                                                                                                cgtcgGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAac      >  1:762990/1‑139 (MQ=255)                                                                                                                                   cgGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAAcaca   <  2:700960/139‑1 (MQ=255)                                                                                                                                    gggTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACAt  <  2:308097/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AATCGATCGAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGCCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACAT  >  NC_000913/594248‑594517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑