Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	847,220	A→G	intergenic (‑101/+38)	glnP ← / ← glnH	glutamine transporter subunit/glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	847,220	0	A	G	100.0%	155.7 / NA	44	intergenic (‑101/+38)	glnP/glnH	glutamine transporter subunit/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/20);  total (24/20)

TGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGT  >  NC_000913/847098‑847346                                                                                                                           |                                                                                                                               tGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTg                                                                                                                <  2:515939/139‑1 (MQ=255)        ccAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTa                                                                                                         <  1:807236/139‑1 (MQ=255)          aGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACa                                                                                                       >  1:794662/1‑139 (MQ=255)                  tGCAAATGTTGTTCCTGTTACCTTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCGCTCCCCATAGGGGGGAGGGAATTGCCAGTGCGTTTTACAGGTGTAGc                                                                                               <  2:268169/139‑1 (MQ=255)                    cATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGt                                                                                             <  2:845726/139‑1 (MQ=255)                            gttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                     <  1:217753/139‑1 (MQ=255)                              tCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCg                                                                                   >  1:820906/1‑139 (MQ=255)                               ccTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCgg                                                                                  >  2:249542/1‑139 (MQ=255)                                 tGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                <  2:549833/139‑1 (MQ=255)                                  gTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTc                                                                               >  1:369718/1‑139 (MQ=255)                                   ttACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTTCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGgtgt                                                                                                  >  2:668104/1‑119 (MQ=255)                                   ttACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGgtgt                                                                                                  <  1:668104/119‑1 (MQ=255)                                       cGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGGTCAGTAc                                                                          >  2:619559/1‑139 (MQ=255)                                          ggtgTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTCATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGa                                                                       >  1:1028767/1‑139 (MQ=255)                                               aaaTAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAt                                                                  <  1:249542/139‑1 (MQ=255)                                                     tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                     <  2:98786/114‑1 (MQ=255)                                                     tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                     >  1:98786/1‑114 (MQ=255)                                                             aGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTc                                                    <  2:427532/139‑1 (MQ=255)                                                               ggTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                  >  2:396921/1‑139 (MQ=255)                                                                    ttaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                     <  1:677285/99‑1 (MQ=255)                                                                    ttaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                     >  2:677285/1‑99 (MQ=255)                                                                          gCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCg                                       >  2:585604/1‑139 (MQ=255)                                                                           ccccGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGt                                      >  2:477810/1‑139 (MQ=255)                                                                                           cgcCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGt                                               >  1:723851/1‑114 (MQ=255)                                                                                           cgcCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGt                                               <  2:723851/114‑1 (MQ=255)                                                                                           cgcCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCa                             >  1:355016/1‑132 (MQ=255)                                                                                           cgcCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCa                             <  2:355016/132‑1 (MQ=255)                                                                                                cTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAcg                 <  1:585604/139‑1 (MQ=255)                                                                                                     ccTAATCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCg            <  1:477810/139‑1 (MQ=255)                                                                                                        aaTCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                     <  2:552141/63‑1 (MQ=255)                                                                                                        aaTCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                     >  1:552141/1‑63 (MQ=255)                                                                                                        aaTCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                >  1:1029864/1‑68 (MQ=255)                                                                                                        aaTCCTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                <  2:1029864/68‑1 (MQ=255)                                                                                                           ccTCTCCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACt      >  2:185211/1‑139 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttat                                                                                          <  2:859254/51‑1 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttat                                                                                          >  1:859254/1‑51 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTg                                                >  1:1021740/1‑93 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTg                                                <  2:1021740/93‑1 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGt  >  1:193560/1‑139 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGt  >  1:436156/1‑139 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGt  >  2:363994/1‑139 (MQ=255)                                                                                                               tcCCCATAGGGGGGAGGGAACTACCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGt  >  2:611986/1‑139 (MQ=255)                                                                                                                      aGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                <  1:369631/54‑1 (MQ=255)                                                                                                                      aGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                >  2:369631/1‑54 (MQ=255)                                                                                                                           |                                                                                                                               TGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGT  >  NC_000913/847098‑847346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑