Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 852,869 C→T *43* (TAG→TAA)  mntS ← Mn(2)‑response protein, MntR‑repressed

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913852,8690CT100.0% 138.4 / NA 44*43* (TAG→TAA) mntSMn(2)‑response protein, MntR‑repressed
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/20);  total (24/20)

CGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGACCT  >  NC_000913/852734‑853004
                                                                                                                                       |                                                                                                                                       
cgccgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTAt                                                                                                                                      >  1:308787/1‑139 (MQ=255)
       cAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCgg                                                                                                                               <  1:839093/139‑1 (MQ=255)
               ctactGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTa                                                                                                                       >  1:240466/1‑139 (MQ=255)
                 actGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATc                                                                                                                     <  2:867292/139‑1 (MQ=255)
                    gACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTtg                                                                                                                  >  1:59748/1‑139 (MQ=255)
                       gAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTtgctg                                                                                                               <  2:259276/139‑1 (MQ=255)
                        aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTtgctgc                                                                                                              >  2:940935/1‑139 (MQ=255)
                             aTCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGttt                                                                                                         >  1:536774/1‑139 (MQ=255)
                                   tGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgtt                                                                                                   >  2:12224/1‑139 (MQ=255)
                                            gATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATc                                                                                          <  1:595229/139‑1 (MQ=255)
                                              ttGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGc                                                                                        <  2:1014895/139‑1 (MQ=255)
                                                    aTCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCacaaca                                                                                  >  2:413629/1‑139 (MQ=255)
                                                    aTCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCacaaca                                                                                  >  1:731859/1‑139 (MQ=255)
                                                         aCAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGGACAACATagag                                                                             >  1:689192/1‑139 (MQ=255)
                                                         aCAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagag                                                                             >  2:198893/1‑139 (MQ=255)
                                                          cAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagaga                                                                            <  1:878698/139‑1 (MQ=255)
                                                                gTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAgcagca                                                                      <  2:308787/139‑1 (MQ=255)
                                                                    ggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTaa                                                                  <  2:224147/139‑1 (MQ=255)
                                                                        tttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATCGAGAGCAGCATTAACCGt                                                              <  1:695218/139‑1 (MQ=255)
                                                                        tttCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGt                                                              <  2:1035800/139‑1 (MQ=255)
                                                                                  aaaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagc                                                                          <  2:898709/117‑1 (MQ=255)
                                                                                  aaaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagc                                                                          >  1:898709/1‑117 (MQ=255)
                                                                                  aaaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAgg                                                    >  1:490173/1‑139 (MQ=255)
                                                                                   aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTttg                                                                                                        <  1:619446/86‑1 (MQ=255)
                                                                                   aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTttg                                                                                                        >  2:619446/1‑86 (MQ=255)
                                                                                   aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                       <  1:598180/103‑1 (MQ=255)
                                                                                   aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGca                                                                                       >  2:598180/1‑103 (MQ=255)
                                                                                   aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagcagc                                                                       >  2:20153/1‑119 (MQ=255)
                                                                                   aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagcagc                                                                       <  1:20153/119‑1 (MQ=255)
                                                                                   aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAggg                                                   >  1:268471/1‑139 (MQ=255)
                                                                                               cTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTATGTTGTTGACCATATCGCACAACATAGAGAGCAACATTAACCGTACTTTAAAGGGAGAATGACTaaa                                       <  1:505815/139‑1 (MQ=255)
                                                                                                tGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAac                                      >  2:268832/1‑139 (MQ=255)
                                                                                                  cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAacac                                    >  1:354703/1‑139 (MQ=255)
                                                                                                  cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAacac                                    >  1:803800/1‑139 (MQ=255)
                                                                                                        aGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTa                                                                                                                       >  2:557129/1‑50 (MQ=255)
                                                                                                        aGCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTa                                                                                                                       <  1:557129/50‑1 (MQ=255)
                                                                                                         gCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATa                             <  2:354703/139‑1 (MQ=255)
                                                                                                         gCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATa                             <  2:490173/139‑1 (MQ=255)
                                                                                                         gCAGGAGTGAAGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATa                             <  1:545642/139‑1 (MQ=255)
                                                                                                                  aaGCGGCGTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTg                    >  2:484132/1‑139 (MQ=255)
                                                                                                                    gcggcgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGaa                  <  1:12224/139‑1 (MQ=255)
                                                                                                                       gcgTACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtc               >  1:755288/1‑139 (MQ=255)
                                                                                                                          tACCGCGACGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcatt            >  1:195649/1‑139 (MQ=255)
                                                                                                                                 aCGCCGTTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGa     >  2:296264/1‑139 (MQ=255)
                                                                                                                                  cgccgtTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                               >  2:496865/1‑112 (MQ=255)
                                                                                                                                  cgccgtTATTTATCGGAAAGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                               <  1:496865/112‑1 (MQ=255)
                                                                                                                                   gccgtTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGAcc   <  1:484132/139‑1 (MQ=255)
                                                                                                                                    ccgtTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGAcct  >  2:383371/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGACCT  >  NC_000913/852734‑853004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑