Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	855,796	A→G	intergenic (‑52/‑167)	ldtB ← / → ybiT	L,D‑transpeptidase linking Lpp to murein/ABC‑F family putative regulatory ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	855,796	0	A	G	100.0%	158.9 / NA	44	intergenic (‑52/‑167)	ldtB/ybiT	L,D‑transpeptidase linking Lpp to murein/ABC‑F family putative regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

ATAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGC  >  NC_000913/855664‑855931                                                                                                                                     |                                                                                                                                        aTAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGaa                                                                                                                                   >  1:151625/1‑139 (MQ=255)     gTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc                                                                                                                               <  1:166065/139‑1 (MQ=255)     gTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  <  2:635662/136‑1 (MQ=255)     gTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  >  1:635662/1‑136 (MQ=255)        aCCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCAtt                                                                                                                            >  2:182270/1‑139 (MQ=255)          cGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat                                                                                                                          >  2:891544/1‑139 (MQ=255)          cGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat                                                                                                                          >  2:522643/1‑139 (MQ=255)               agGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGtt                                                                                                                     <  1:323769/139‑1 (MQ=255)                 gCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCa                                                                                                                   >  2:802167/1‑139 (MQ=255)                          cAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTc                                                                                                          <  2:151625/139‑1 (MQ=255)                           aaaaGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTca                                                                                                         <  1:182270/139‑1 (MQ=255)                               gCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGt                                                                                                     <  2:105246/139‑1 (MQ=255)                                       caGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg                                                                                             <  2:635572/139‑1 (MQ=255)                                                 cGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGa                                                                                   >  2:985209/1‑139 (MQ=255)                                                  gCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  <  1:110801/91‑1 (MQ=255)                                                  gCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  >  2:110801/1‑91 (MQ=255)                                                    tGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg                                                                                             >  1:142522/1‑126 (MQ=255)                                                    tGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg                                                                                             <  2:142522/126‑1 (MQ=255)                                                      cGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATca                                                                              <  1:660741/139‑1 (MQ=255)                                                         ataatGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  <  1:200679/84‑1 (MQ=255)                                                         ataatGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  >  2:200679/1‑84 (MQ=255)                                                                        tCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCt                                                            <  1:891544/139‑1 (MQ=255)                                                                        tCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCt                                                            <  2:790420/139‑1 (MQ=255)                                                                         cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat                                                                                                                          >  1:918752/1‑76 (MQ=255)                                                                         cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat                                                                                                                          <  2:918752/76‑1 (MQ=255)                                                                         cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTcaca                                                                                                       >  1:971291/1‑95 (MQ=255)                                                                         cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTcaca                                                                                                       <  2:971291/95‑1 (MQ=255)                                                                         cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt                                                           <  2:379596/139‑1 (MQ=255)                                                                         cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt                                                           >  1:829343/1‑139 (MQ=255)                                                                         cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt                                                           <  2:505900/139‑1 (MQ=255)                                                                              tCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGACATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAgtg                                                      <  2:294671/139‑1 (MQ=255)                                                                              tCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAgtg                                                      <  2:19190/139‑1 (MQ=255)                                                                                  gTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGa                                                  >  2:478195/1‑139 (MQ=255)                                                                                        cGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTaa                                            >  2:398234/1‑139 (MQ=255)                                                                                                 gtgCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGc                                   >  1:133498/1‑139 (MQ=255)                                                                                                       ggCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGAtc                             >  1:886360/1‑139 (MQ=255)                                                                                                       ggCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGAtc                             <  2:886360/139‑1 (MQ=255)                                                                                                                    gTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACtt                <  2:829343/139‑1 (MQ=255)                                                                                                                              ttAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAAt      >  1:802125/1‑139 (MQ=255)                                                                                                                               tAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATg     >  2:330215/1‑139 (MQ=255)                                                                                                                                 gagTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcg   <  1:330215/139‑1 (MQ=255)                                                                                                                                  agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcgc  >  2:186962/1‑139 (MQ=255)                                                                                                                                  agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcgc  <  2:979103/139‑1 (MQ=255)                                                                                                                                  agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGATTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcgc  >  1:979103/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        ATAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGC  >  NC_000913/855664‑855931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑