Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	896,680	A→G	T183A (ACC→GCC)	potH →	putrescine ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	896,680	0	A	G	100.0%	212.1 / NA	59	T183A (ACC→GCC)	potH	putrescine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)

CCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAA  >  NC_000913/896553‑896815                                                                                                                                |                                                                                                                                        ccTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGcc                                                                                                                              >  2:401306/1‑139 (MQ=255)   tGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGt                                                                                                                            >  2:288528/1‑139 (MQ=255)   tGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGt                                                                                                                            >  2:564394/1‑139 (MQ=255)              aCCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCa                                                                                                                 <  1:1011675/139‑1 (MQ=255)               ccTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCAt                                                                                                                <  2:414009/139‑1 (MQ=255)               ccTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCAt                                                                                                                >  2:580414/1‑139 (MQ=255)                  cGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCa                                                                                                                 >  1:542046/1‑135 (MQ=255)                  cGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCa                                                                                                                 <  2:542046/135‑1 (MQ=255)                     ttCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGtt                                                                                                            <  2:273715/137‑1 (MQ=255)                     ttCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGtt                                                                                                            >  1:273715/1‑137 (MQ=255)                          aTCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCtt                                                                                                     <  2:342975/139‑1 (MQ=255)                            ccGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTAc                                                                                                   >  1:545329/1‑139 (MQ=255)                               cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                >  1:982803/1‑139 (MQ=255)                               cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                >  1:911759/1‑139 (MQ=255)                               cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  1:580414/139‑1 (MQ=255)                               cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  2:982803/139‑1 (MQ=255)                                    aTGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAAt                                                                                                                                    >  1:643345/1‑98 (MQ=255)                                    aTGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAAt                                                                                                                                    <  2:643345/98‑1 (MQ=255)                                              gggAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACGATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 <  1:863702/139‑1 (MQ=255)                                              gggAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 <  2:833337/139‑1 (MQ=255)                                               ggAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGc                                                                                <  2:323059/139‑1 (MQ=255)                                                gAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGGATGCCAATCTGGCCGTTTATATCgg                                                                                                                   >  2:674679/1‑103 (MQ=255)                                                gAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCgg                                                                                                                   <  1:674679/103‑1 (MQ=255)                                                   tatTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGAt                                                                            <  1:216996/139‑1 (MQ=255)                                                     ttAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  1:486831/117‑1 (MQ=255)                                                     ttAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGAttt                                                                          <  1:401306/139‑1 (MQ=255)                                                     ttAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACATg                                                                                                >  2:486831/1‑117 (MQ=255)                                                      tAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  2:148827/116‑1 (MQ=255)                                                      tAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                >  1:148827/1‑116 (MQ=255)                                                      tAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTta                                                                         >  1:67447/1‑139 (MQ=255)                                                             caacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCGAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACcgcg                                                                  >  2:607589/1‑139 (MQ=255)                                                              aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTatat                                                                                                                      >  1:55382/1‑86 (MQ=255)                                                              aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTatat                                                                                                                      <  2:55382/86‑1 (MQ=255)                                                              aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGcc                                                                                              >  2:962684/1‑110 (MQ=255)                                                              aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGcc                                                                                              <  1:962684/110‑1 (MQ=255)                                                              aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGt                                                                 >  1:877038/1‑139 (MQ=255)                                                              aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGt                                                                 >  2:55940/1‑139 (MQ=255)                                                               acGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGtt                                                                >  2:236228/1‑139 (MQ=255)                                                                  gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  2:917044/139‑1 (MQ=255)                                                                  gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  2:911759/139‑1 (MQ=255)                                                                  gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  1:55940/139‑1 (MQ=255)                                                                  gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  1:564394/139‑1 (MQ=255)                                                                     tgCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCg                                                          >  2:985887/1‑139 (MQ=255)                                                                          aataatTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTg                                                     >  2:637924/1‑139 (MQ=255)                                                                           ataatTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTa                                                                                         <  2:411309/102‑1 (MQ=255)                                                                           ataatTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTa                                                                                         >  1:411309/1‑102 (MQ=255)                                                                                tttCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGattatt                                               <  1:906299/139‑1 (MQ=255)                                                                                      ctgTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCtgg                                         >  2:95390/1‑139 (MQ=255)                                                                                                 ggTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATTTTGGCATTGTTTGCGCTTACGTGCCGTTTATGGTATTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAgcg                              <  2:258038/139‑1 (MQ=255)                                                                                                     aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCt                                                                                                      <  1:1004125/63‑1 (MQ=255)                                                                                                     aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCt                                                                                                      >  2:1004125/1‑63 (MQ=255)                                                                                                     aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 <  1:797736/84‑1 (MQ=255)                                                                                                     aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 >  2:797736/1‑84 (MQ=255)                                                                                                     aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGattatt                                               <  1:81143/118‑1 (MQ=255)                                                                                                     aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGattatt                                               >  2:81143/1‑118 (MQ=255)                                                                                                     aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTgg                          >  1:116735/1‑139 (MQ=255)                                                                                                                            gCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             >  1:979283/1‑81 (MQ=255)                                                                                                                            gCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  2:979283/81‑1 (MQ=255)                                                                                                                             cATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGaaaa  <  1:95390/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                        CCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAA  >  NC_000913/896553‑896815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑