Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 956,917 A→G E52E (GAA→GAG)  ycaP → UPF0702 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913956,9170AG100.0% 273.6 / NA 74E52E (GAA→GAG) ycaPUPF0702 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/38);  total (36/38)

CCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAAGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTTATACCGCCTGGTAATGTGGTTGATGGCG  >  NC_000913/956782‑957049
                                                                                                                                       |                                                                                                                                    
ccGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGtt                                                                                                                                   >  1:279276/1‑139 (MQ=255)
   tATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTa                                                                                                                                >  1:719954/1‑139 (MQ=255)
   tATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTa                                                                                                                                >  1:740293/1‑139 (MQ=255)
      ggCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTCTAATc                                                                                                                             >  1:667276/1‑139 (MQ=255)
        cATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCAt                                                                                                                           >  1:244523/1‑139 (MQ=255)
            tGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTg                                                                                                                       <  2:771316/139‑1 (MQ=255)
                 aaGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCt                                                                                                                  <  2:740293/139‑1 (MQ=255)
                  aGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGTGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTg                                                                                                                 >  1:335919/1‑139 (MQ=255)
                      ccTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGTGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGAt                                                                                                             <  2:335919/139‑1 (MQ=255)
                      ccTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGAt                                                                                                             >  2:452172/1‑139 (MQ=255)
                       cTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATc                                                                                                            <  2:279276/139‑1 (MQ=255)
                         tttGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAg                                                                                                          >  1:331793/1‑139 (MQ=255)
                          ttGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAgc                                                                                                         <  2:937428/139‑1 (MQ=255)
                          ttGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAgc                                                                                                         <  2:331793/139‑1 (MQ=255)
                             aTTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAgcggc                                                                                                      <  2:162919/139‑1 (MQ=255)
                              tttCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTaa                                                                                                                               >  2:154600/1‑113 (MQ=255)
                              tttCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTaa                                                                                                                               <  1:154600/113‑1 (MQ=255)
                                          aGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCg                                                                                         <  2:479299/139‑1 (MQ=255)
                                           gTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGt                                                                                        >  1:1029336/1‑139 (MQ=255)
                                            ttGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGtt                                                                                       <  2:213323/139‑1 (MQ=255)
                                            ttGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGtt                                                                                       >  2:729055/1‑139 (MQ=255)
                                            ttGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTCTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGtt                                                                                       <  2:667276/139‑1 (MQ=255)
                                               cACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTa                                                                                    <  1:729055/139‑1 (MQ=255)
                                                         tCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGAtgtg                                                                          <  1:649708/139‑1 (MQ=255)
                                                              ataCCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAg                                                                                                          <  2:576613/102‑1 (MQ=255)
                                                              ataCCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAg                                                                                                          >  1:576613/1‑102 (MQ=255)
                                                                aCCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATgg                                                                   >  1:421485/1‑139 (MQ=255)
                                                                    ttGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTatgatgat                                                                             <  1:54074/125‑1 (MQ=255)
                                                                    ttGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTatgatgat                                                                             >  2:54074/1‑125 (MQ=255)
                                                                     tGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCg                                                                                         >  2:243186/1‑112 (MQ=255)
                                                                     tGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCg                                                                                         <  1:243186/112‑1 (MQ=255)
                                                                     tGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCg                                                              >  1:702150/1‑139 (MQ=255)
                                                                     tGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCg                                                              >  1:405655/1‑139 (MQ=255)
                                                                      gTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGCTGATGTGCCGATGGTCTCgg                                                             <  1:716058/139‑1 (MQ=255)
                                                                       tACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGt                                                            >  1:598437/1‑139 (MQ=255)
                                                                           ggTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACtt                                                        >  1:669175/1‑139 (MQ=255)
                                                                            gTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTa                                                       >  1:976843/1‑139 (MQ=255)
                                                                            gTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTa                                                       >  2:519592/1‑139 (MQ=255)
                                                                            gTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTa                                                       >  2:1041853/1‑139 (MQ=255)
                                                                            gTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTa                                                       >  1:1053802/1‑139 (MQ=255)
                                                                            gTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTa                                                       >  1:541230/1‑139 (MQ=255)
                                                                                ttttGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGt                                                   <  1:363824/139‑1 (MQ=255)
                                                                                 tttGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGTCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTc                                                  >  1:416780/1‑139 (MQ=255)
                                                                                 tttGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCt                                                                                                                  <  2:655180/75‑1 (MQ=255)
                                                                                 tttGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCt                                                                                                                  >  1:655180/1‑75 (MQ=255)
                                                                                 tttGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTc                                                  >  2:188467/1‑139 (MQ=255)
                                                                                      tCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACg                                                                                                                    >  2:772746/1‑68 (MQ=255)
                                                                                      tCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACg                                                                                                                    <  1:772746/68‑1 (MQ=255)
                                                                                       ccTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGt                                                                  >  2:1040178/1‑117 (MQ=255)
                                                                                       ccTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGt                                                                  <  1:1040178/117‑1 (MQ=255)
                                                                                         tCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTAc                                          <  2:174668/139‑1 (MQ=255)
                                                                                              aTGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGTGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTgg                                     <  2:719954/139‑1 (MQ=255)
                                                                                              aTGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTgg                                     <  2:1029336/139‑1 (MQ=255)
                                                                                              aTGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTgg                                     <  1:452172/139‑1 (MQ=255)
                                                                                              aTGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTgg                                     <  1:912142/139‑1 (MQ=255)
                                                                                                  ccGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCgtt                                 >  1:174826/1‑139 (MQ=255)
                                                                                                        gACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTtata                           <  2:405655/139‑1 (MQ=255)
                                                                                                        gACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTtata                           <  2:1053802/139‑1 (MQ=255)
                                                                                                        gACGCGGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTtata                           <  2:702150/139‑1 (MQ=255)
                                                                                                           gcgGTGTGCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTTATACCg                        <  1:188467/139‑1 (MQ=255)
                                                                                                                gtgCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTTATACCGCCTgg                   <  2:244523/139‑1 (MQ=255)
                                                                                                                 tgCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAgc                                                                                                         >  1:610953/1‑52 (MQ=255)
                                                                                                                 tgCGGCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAgc                                                                                                         <  2:610953/52‑1 (MQ=255)
                                                                                                                     gCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTcc                                                                >  1:674063/1‑89 (MQ=255)
                                                                                                                     gCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTcc                                                                <  2:674063/89‑1 (MQ=255)
                                                                                                                     gCAGATGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTTATACCGCCTGGTAAtg              >  2:411350/1‑139 (MQ=255)
                                                                                                                         aTGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTatg                                                                                  >  1:126494/1‑67 (MQ=255)
                                                                                                                         aTGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTatg                                                                                  <  2:126494/67‑1 (MQ=255)
                                                                                                                         aTGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCgg                                                             <  2:478607/88‑1 (MQ=255)
                                                                                                                         aTGTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCgg                                                             >  1:478607/1‑88 (MQ=255)
                                                                                                                           gTCGCTGTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTTATACCGCCTGGTAATGTGGTTg        <  1:721181/139‑1 (MQ=255)
                                                                                                                                 gTTTGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTTATACCGCCTGGTAATGTGGTTGATGgcg  <  2:416780/139‑1 (MQ=255)
                                                                                                                                  tttGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTcc                                                                >  1:137310/1‑76 (MQ=255)
                                                                                                                                  tttGAGGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTcc                                                                <  2:137310/76‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CCGTATGGCATTTGATAAAGTGCCTTTTGATTTCCTTGGCGAAGTTGCACTACGTAGTCTTTATACCTTTGTACTGGTCTTTTTGTTCCTCAAAATGACCGGAAGACGCGGTGTGCGGCAGATGTCGCTGTTTGAAGTTTTAATCATTCTGACGCTGGGATCAGCGGCGGGAGATGTGGCGTTTTATGATGATGTGCCGATGGTCCCGGTACTTATCGTCTTTATTACTCTGGCGTTGTTATACCGCCTGGTAATGTGGTTGATGGCG  >  NC_000913/956782‑957049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑