Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,032,528	G→T	G130G (GGG→GGT)	hyaA →	hydrogenase 1, small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,032,528	0	G	T	100.0%	80.5 / NA	27	G130G (GGG→GGT)	hyaA	hydrogenase 1, small subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/11);  total (16/11)

TCCCTCGATTACGACGATACTTTGATGGCTGCCGCCGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGGATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCCGCG  >  NC_000913/1032394‑1032645                                                                                                                                       |                                                                                                                      tCCCTCGATTACGACGATACTTTGATGGCTGCCGCCGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGt                                                                                                                   <  2:122414/139‑1 (MQ=255)                               gccgccGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTg                                                                                     >  2:97752/1‑139 (MQ=255)                                              gCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTg                                                                      >  2:518789/1‑139 (MQ=255)                                                ggAAGAAGTCTTTGAAGACATCATCACACAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGcc                                                                    <  2:687341/139‑1 (MQ=255)                                                            tGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGcc                                                        <  1:628116/139‑1 (MQ=255)                                                                  catcatCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGcgcg                                                  >  2:834590/1‑139 (MQ=255)                                                                   atcatcACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGa                                                 >  2:126435/1‑139 (MQ=255)                                                                             aaTACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTg                                       >  2:1027130/1‑139 (MQ=255)                                                                               tACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTggg                                     >  2:381352/1‑139 (MQ=255)                                                                               tACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTggg                                     >  2:1008252/1‑139 (MQ=255)                                                                                   aTGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAAc                                 <  1:25592/139‑1 (MQ=255)                                                                                        aaaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            <  1:834590/139‑1 (MQ=255)                                                                                        aaaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            <  1:381352/139‑1 (MQ=255)                                                                                         aaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATcagcag                                                                                                      <  1:821647/64‑1 (MQ=255)                                                                                         aaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATcagcag                                                                                                      >  2:821647/1‑64 (MQ=255)                                                                                         aaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcgc                           >  2:902391/1‑139 (MQ=255)                                                                                                ccTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTgg                    >  1:950143/1‑139 (MQ=255)                                                                                                     cAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTg               >  2:833173/1‑139 (MQ=255)                                                                                                                  tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            <  1:607359/113‑1 (MQ=255)                                                                                                                  tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            >  2:607359/1‑113 (MQ=255)                                                                                                                  tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCcgcg  >  2:147305/1‑139 (MQ=255)                                                                                                                  tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCcgcg  >  2:358610/1‑139 (MQ=255)                                                                                                                  tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCcgcg  <  1:1027130/139‑1 (MQ=255)                                                                                                                       cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            <  1:988181/108‑1 (MQ=255)                                                                                                                       cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            <  2:502697/108‑1 (MQ=255)                                                                                                                       cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            >  1:502697/1‑108 (MQ=255)                                                                                                                       cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg                            >  2:988181/1‑108 (MQ=255)                                                                                                                                       |                                                                                                                      TCCCTCGATTACGACGATACTTTGATGGCTGCCGCCGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGGATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCCGCG  >  NC_000913/1032394‑1032645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑