Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,096,689	C→T	R283W (CGG→TGG)	ycdU →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,096,689	0	C	T	100.0%	158.5 / NA	50	R283W (CGG→TGG)	ycdU	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/22);  total (28/22)

TGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGCGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTATTTCCGTTGGGGGCATTTTAATA  >  NC_000913/1096558‑1096824                                                                                                                                    |                                                                                                                                        tGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTg                                                                                                                                  >  2:471180/1‑139 (MQ=255)             gTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTa                                                                                                                      >  2:830437/1‑139 (MQ=255)             gTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTa                                                                                                                      >  1:224424/1‑139 (MQ=255)               gAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTAtt                                                                                                                    <  2:224424/139‑1 (MQ=255)                 aaaTGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTAtttt                                                                                                                  <  2:820459/139‑1 (MQ=255)                    tGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTa                                                                                                               <  2:346969/139‑1 (MQ=255)                    tGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTa                                                                                                               <  2:995145/139‑1 (MQ=255)                    tGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTa                                                                                                               <  2:1015104/139‑1 (MQ=255)                      cGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  1:707654/139‑1 (MQ=255)                      cGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  1:732526/139‑1 (MQ=255)                      cGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  1:830437/139‑1 (MQ=255)                      cGTTGTCGACCTTTTTGGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  1:343708/139‑1 (MQ=255)                       gTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTActc                                                                                                            <  2:39958/139‑1 (MQ=255)                          gTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGc                                                                                                         >  2:621321/1‑139 (MQ=255)                                     ttGCCAAGCCGCTTTGGCTTCGGTTATTCTTATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGCTTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTg                                                                                              >  2:227125/1‑139 (MQ=255)                                            gCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAAtt                                                                                       <  2:789201/139‑1 (MQ=255)                                                       ttCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTAtttttt                                                                            >  1:809334/1‑139 (MQ=255)                                                       ttCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTAtttttt                                                                            <  2:29835/139‑1 (MQ=255)                                                                    atTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  2:97383/93‑1 (MQ=255)                                                                    atTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             >  1:97383/1‑93 (MQ=255)                                                                     ttGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTCTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTcc                                                              >  2:703485/1‑139 (MQ=255)                                                                        cTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCctct                                                           >  2:875045/1‑139 (MQ=255)                                                                          ggTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGc                                                                                                                         >  1:471994/1‑75 (MQ=255)                                                                          ggTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGc                                                                                                                         <  2:471994/75‑1 (MQ=255)                                                                          ggTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  2:721352/87‑1 (MQ=255)                                                                          ggTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             >  1:721352/1‑87 (MQ=255)                                                                          ggTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTgg                                                         >  2:519923/1‑139 (MQ=255)                                                                             tATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCAt                                                      >  1:586969/1‑139 (MQ=255)                                                                                       aTGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTa                                            >  1:729943/1‑139 (MQ=255)                                                                                         gtgGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTatg                                          >  2:89364/1‑139 (MQ=255)                                                                                            gATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTatgatg                                       <  2:809334/139‑1 (MQ=255)                                                                                            gATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTatgatg                                       >  2:406504/1‑139 (MQ=255)                                                                                            gATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTatgatg                                       >  2:960648/1‑139 (MQ=255)                                                                                                 cGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCAtg                                  >  2:69853/1‑139 (MQ=255)                                                                                                 cGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCAtg                                  >  2:770533/1‑139 (MQ=255)                                                                                                 cGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCAtg                                  >  1:65584/1‑139 (MQ=255)                                                                                                  gCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCTTCTGGCATAACGATTTTATGATGGCAtgt                                 >  1:295773/1‑139 (MQ=255)                                                                                                      gtgtCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             >  1:805577/1‑59 (MQ=255)                                                                                                      gtgtCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  2:805577/59‑1 (MQ=255)                                                                                                      gtgtCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACa                             >  1:542197/1‑139 (MQ=255)                                                                                                       tgtCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             <  2:88315/58‑1 (MQ=255)                                                                                                       tgtCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTAct                                                                                                             >  1:88315/1‑58 (MQ=255)                                                                                                       tgtCGGTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACAt                            >  2:454136/1‑139 (MQ=255)                                                                                                           ggTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTAt                        >  1:273605/1‑139 (MQ=255)                                                                                                            gTATTGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTAtt                       <  1:89364/139‑1 (MQ=255)                                                                                                                tGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTatga                                         >  1:347354/1‑117 (MQ=255)                                                                                                                tGGCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTatga                                         <  2:347354/117‑1 (MQ=255)                                                                                                                  gCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTATTTCCGtt                 <  2:542197/139‑1 (MQ=255)                                                                                                                  gCAACGCTTGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTACTCTGGCATAACGATATTATGATGGCATGTGACATGTATTTCCGtt                 >  2:8208/1‑139 (MQ=255)                                                                                                                         ttGTAGAATGGTGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTATTTCCGTTGGGGGCa          <  1:770533/139‑1 (MQ=255)                                                                                                                                 tggtggATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTATTTCCGTTGGGGGCATTTtaata  >  2:265001/1‑139 (MQ=255)                                                                                                                                 tggtggATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTATTTCCGTTGGGGGCATTTtaata  >  1:495086/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        TGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATTGCTGGTTATTGAAGTGATGTGGATTACGCTGGTGTCGGTATTGGCAACGCTTGTAGAATGGCGGATTTGGTTTGAAGCCTATTTTTTACTCTGCTATGTACCGTGCTTAATTTACTATTTTTTCTATTGTCGATTCCTCTGGCATAACGATTTTATGATGGCATGTGACATGTATTTCCGTTGGGGGCATTTTAATA  >  NC_000913/1096558‑1096824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑