Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,600	G→A	*147* (TAG→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,600	0	G	A	100.0%	135.4 / NA	42	*147* (TAG→TAA)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

TAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTA  >  NC_000913/1245465‑1245730                                                                                                                                        |                                                                                                                                   taGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTAAAACACTTACCCGTAGAAATGACTCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAaaaa                                                                                                                                 <  2:674450/139‑1 (MQ=255)  aGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaa                                                                                                                                >  2:234036/1‑139 (MQ=255)    ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                              >  2:100578/1‑139 (MQ=255)       aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                                           >  1:904158/1‑139 (MQ=255)       aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                                           <  1:379010/139‑1 (MQ=255)            ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                                      <  2:70034/139‑1 (MQ=255)             gCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTa                                                                                                                     <  1:856893/139‑1 (MQ=255)              cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAg                                                                                                                        <  2:908678/135‑1 (MQ=255)              cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAg                                                                                                                        >  1:908678/1‑135 (MQ=255)              cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTAt                                                                                                                    >  2:1042113/1‑139 (MQ=255)                   aTCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGgtgt                                                                                                               >  2:140243/1‑139 (MQ=255)                        cGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCt                                                                                                          <  2:904158/139‑1 (MQ=255)                            tgatgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCg                                                                                                      <  1:140243/139‑1 (MQ=255)                                               aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACa                                                                                                   >  2:66049/1‑123 (MQ=255)                                               aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGAACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACa                                                                                                   <  1:66049/123‑1 (MQ=255)                                                     aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                             >  1:40500/1‑139 (MQ=255)                                                         aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCAt                                                                                                                          <  1:807184/90‑1 (MQ=255)                                                         aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCAt                                                                                                                          >  2:807184/1‑90 (MQ=255)                                                           gACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGtt                                                                       >  2:296066/1‑139 (MQ=255)                                                                 aTCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                >  2:49003/1‑108 (MQ=255)                                                                 aTCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                <  1:49003/108‑1 (MQ=255)                                                                              aCGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                    <  1:296066/139‑1 (MQ=255)                                                                                 tATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAg                                                 >  1:845716/1‑139 (MQ=255)                                                                                     gCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGgtgt                                             >  2:115007/1‑139 (MQ=255)                                                                                        aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTggg                                          <  2:1033665/139‑1 (MQ=255)                                                                                            tttGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtgt                                      <  1:115007/139‑1 (MQ=255)                                                                                                  cAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                <  1:108544/139‑1 (MQ=255)                                                                                                  cAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                <  1:234036/139‑1 (MQ=255)                                                                                                  cAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                >  1:349455/1‑139 (MQ=255)                                                                                                     tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGa                                                                               >  2:440132/1‑89 (MQ=255)                                                                                                     tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGa                                                                               <  1:440132/89‑1 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa                        >  1:599264/1‑139 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACACCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcg                                                          >  1:526527/1‑105 (MQ=255)                                                                                                          cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACACCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcg                                                          <  2:526527/105‑1 (MQ=255)                                                                                                               cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                >  2:454129/1‑126 (MQ=255)                                                                                                               cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                <  1:454129/126‑1 (MQ=255)                                                                                                                  gACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAg                                                                                   <  2:94174/72‑1 (MQ=255)                                                                                                                  gACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAg                                                                                   >  1:94174/1‑72 (MQ=255)                                                                                                                        aaaaaCCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTg          <  1:1042113/139‑1 (MQ=255)                                                                                                                            aCCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGa      >  2:735831/1‑139 (MQ=255)                                                                                                                               gTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                <  1:425421/46‑1 (MQ=255)                                                                                                                               gTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                >  2:425421/1‑46 (MQ=255)                                                                                                                                tCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTa  <  1:565430/139‑1 (MQ=255)                                                                                                                                 cTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAACCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGt                    <  2:557865/120‑1 (MQ=255)                                                                                                                                 cTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAACCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGt                    >  1:557865/1‑120 (MQ=255)                                                                                                                                        |                                                                                                                                   TAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTA  >  NC_000913/1245465‑1245730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑