Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,274,973 C→T T397M (ACG→ATG)  ychO → putative invasin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,274,9730CT100.0% 79.2 / NA 26T397M (ACG→ATG) ychOputative invasin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

TAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCAA  >  NC_000913/1274841‑1275108
                                                                                                                                    |                                                                                                                                       
tAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCggg                                                                                                                                   >  2:608115/1‑139 (MQ=255)
tAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCggg                                                                                                                                   >  1:859918/1‑139 (MQ=255)
          tCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGcc                                                                                                                         <  2:375963/139‑1 (MQ=255)
                     cgccgTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGa                                                                                                              >  1:156400/1‑139 (MQ=255)
                            ggATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTgg                                                                                                       <  2:408251/139‑1 (MQ=255)
                               tCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg                                                                                                    <  1:608115/139‑1 (MQ=255)
                                         ggCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGc                                                                                          >  1:94736/1‑139 (MQ=255)
                                                cAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATGAAGTTTGATGCCGGGCGCACAAGCCAATAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTg                                                                                   >  1:636508/1‑139 (MQ=255)
                                                   taccGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGACCATGCCTGACTGGCa                                                                                <  2:706358/137‑1 (MQ=255)
                                                      cGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGaa                                                                             <  2:156400/139‑1 (MQ=255)
                                                          gAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACggg                                                                         >  1:743506/1‑139 (MQ=255)
                                                                 cAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAgggg                                                                  <  2:443746/139‑1 (MQ=255)
                                                                     tCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGgcaag                                                              >  2:177069/1‑139 (MQ=255)
                                                                                tACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAgggg                                                                  <  1:278884/124‑1 (MQ=255)
                                                                                tACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAgggg                                                                  >  2:278884/1‑124 (MQ=255)
                                                                                      aTTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGt                                             <  2:579324/139‑1 (MQ=255)
                                                                                        tCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGa                                                 <  1:969691/133‑1 (MQ=255)
                                                                                        tCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGa                                                 >  2:969691/1‑133 (MQ=255)
                                                                                                tGATTTGGCAGGGCGATATGCAGATATTAATTTGGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGCCGCTGATCATGCCTGACTGGCAGAAGGGGGAAGGAGCAAGCAATCACTGGCGATTGCCTgtggtggt                                   <  2:950440/139‑1 (MQ=255)
                                                                                                           ggCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAAcc                        >  1:778806/1‑139 (MQ=255)
                                                                                                              gATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAgg                     <  2:859918/139‑1 (MQ=255)
                                                                                                                                 tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGgcaagcaa                                                           <  1:595420/82‑1 (MQ=255)
                                                                                                                                 tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGgcaagcaa                                                           >  2:595420/1‑82 (MQ=255)
                                                                                                                                 tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCaa  >  1:396250/1‑139 (MQ=255)
                                                                                                                                 tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCaa  >  1:344312/1‑139 (MQ=255)
                                                                                                                                 tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCaa  >  2:85828/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCAA  >  NC_000913/1274841‑1275108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: