Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,788	C→T	A281T (GCT→ACT)	sapD ←	antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,788	0	C	T	100.0%	107.7 / NA	34	A281T (GCT→ACT)	sapD	antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

TTCAGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTC  >  NC_000913/1352652‑1352914                                                                                                                                         |                                                                                                                               ttCAGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAgtgc                                                                                                                              >  2:1038697/1‑139 (MQ=255)     gCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCggg                                                                                                                          >  1:166736/1‑139 (MQ=255)      cGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGACAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGc                                                                                                                         >  1:433272/1‑139 (MQ=255)           aaTGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGt                                                                                                                    >  1:358747/1‑139 (MQ=255)                             gatTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATg                                                                                                  >  1:598955/1‑139 (MQ=255)                              atTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCCTTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGc                                                                                                 >  2:988723/1‑139 (MQ=255)                                    cGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATc                                                                                           >  2:897275/1‑139 (MQ=255)                                     gCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATcg                                                                                          >  2:764541/1‑139 (MQ=255)                                             cAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCg                                                                                  <  1:826458/139‑1 (MQ=255)                                             cAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTACCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCg                                                                                  <  1:454692/139‑1 (MQ=255)                                               aaCGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGaa                                                                                <  2:358747/139‑1 (MQ=255)                                                   tGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCt                                                                            >  1:547581/1‑139 (MQ=255)                                                      cGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGt                                                                         >  2:814271/1‑139 (MQ=255)                                                                   tctcGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCa                                                            >  2:717960/1‑139 (MQ=255)                                                                    ctcGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAg                                                           <  1:814271/139‑1 (MQ=255)                                                                      cGTTGTGCATACGGGCAACGTGGCCCCAGACGACAGCCATTCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCg                                                                                  <  2:597236/114‑1 (MQ=255)                                                                      cGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCg                                                                                  >  1:597236/1‑114 (MQ=255)                                                                         tgtgCATACGGGCAACGTGGCCCCAGACGCCAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCt                                                      <  1:1038697/139‑1 (MQ=255)                                                                         tgtgCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGTGCCt                                                      <  1:764541/139‑1 (MQ=255)                                                                         tgtgCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCt                                                      <  2:433272/139‑1 (MQ=255)                                                                         tgtgCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCt                                                      <  1:229733/139‑1 (MQ=255)                                                                            gCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTggg                                                   <  1:717960/139‑1 (MQ=255)                                                                               tACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAg                                                           <  1:842028/128‑1 (MQ=255)                                                                               tACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAg                                                           >  2:842028/1‑128 (MQ=255)                                                                                     cAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGgat                                          >  2:254772/1‑139 (MQ=255)                                                                                       aCGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGgatga                                        >  1:280835/1‑139 (MQ=255)                                                                                        cGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGgatgat                                       <  2:598955/139‑1 (MQ=255)                                                                                                             aTCGGTAACTGTTCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCt                  >  2:844474/1‑139 (MQ=255)                                                                                                                        ttCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGc                                                                                                            <  2:559573/38‑1 (MQ=255)                                                                                                                        ttCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGc                                                                                                            >  1:559573/1‑38 (MQ=255)                                                                                                                        ttCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGGGACTTTTATGCGACATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGgc          >  1:705726/1‑136 (MQ=255)                                                                                                                        ttCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGcgcg       <  2:280835/139‑1 (MQ=255)                                                                                                                        ttCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGcgcg       >  2:934064/1‑139 (MQ=255)                                                                                                                        ttCCAGCAGTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGACATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGgc          <  2:705726/136‑1 (MQ=255)                                                                                                                             gcagTGGGATAGTGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTc  >  1:1002123/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                               TTCAGCGGGAAATGACAGGCATAGAGATGATTTTTCGCCCCCGTCAAACGTGGCGTCACAATGCATTCTCGTTGTGCATACGGGCAACGTGGCCCCAGACGACAACCAATCGGTAACTGTTCCAGCAGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTC  >  NC_000913/1352652‑1352914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑