Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	131,552	T→C	intergenic (‑292/‑63)	yacH ← / → acnB	DUF3300 family protein/aconitate hydratase 2, aconitase B, 2‑methyl‑cis‑aconitate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	131,552	0	T	C	100.0%	145.4 / NA	40	intergenic (‑292/‑63)	yacH/acnB	DUF3300 family protein/aconitate hydratase 2, aconitase B, 2‑methyl‑cis‑aconitate hydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

AAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGATTGCGCCCAAACCCCTGGATGC  >  NC_000913/131420‑131685                                                                                                                                     |                                                                                                                                      aaaCAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTcaca                                                                                                                                 <  1:1017069/139‑1 (MQ=255)        ctctAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGt                                                                                                                          <  2:701440/139‑1 (MQ=255)                     ttAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGcc                                                                                                             >  2:154418/1‑139 (MQ=255)                        aCGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGc                                                                                                          <  1:914567/139‑1 (MQ=255)                          gATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        <  2:41542/139‑1 (MQ=255)                          gATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        <  2:109121/139‑1 (MQ=255)                          gATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        <  1:99610/139‑1 (MQ=255)                            ttCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCa                                                                                                      >  2:395776/1‑139 (MQ=255)                             tCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCAc                                                                                                     >  2:762479/1‑139 (MQ=255)                             tCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCAc                                                                                                     >  1:85080/1‑139 (MQ=255)                                             gTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCg                                                                                     <  1:762479/139‑1 (MQ=255)                                               tGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCgag                                                                                   >  1:388821/1‑139 (MQ=255)                                               tGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCATACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGc                                                                                                          <  1:689357/116‑1 (MQ=255)                                               tGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTAGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGc                                                                                                          >  2:689357/1‑116 (MQ=255)                                                 cTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCgagga                                                                                 >  1:646962/1‑139 (MQ=255)                                                        gTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACcgt                                                                          <  1:720588/139‑1 (MQ=255)                                                                aTTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCa                                                                                                      <  2:845634/103‑1 (MQ=255)                                                                aTTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCa                                                                                                      >  1:845634/1‑103 (MQ=255)                                                                aTTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTag                                                                  >  2:382838/1‑139 (MQ=255)                                                                        cTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGc                                                                     <  1:723524/128‑1 (MQ=255)                                                                        cTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGc                                                                     >  2:723524/1‑128 (MQ=255)                                                                          cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTcaca                                                                                                                                 <  1:877267/66‑1 (MQ=255)                                                                          cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTcaca                                                                                                                                 >  2:877267/1‑66 (MQ=255)                                                                          cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGa                                                                                                >  1:311773/1‑99 (MQ=255)                                                                          cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGa                                                                                                <  2:311773/99‑1 (MQ=255)                                                                          cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCg                                                                                     >  1:400574/1‑110 (MQ=255)                                                                          cGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCg                                                                                     <  2:400574/110‑1 (MQ=255)                                                                            tCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTa                                                      <  1:619794/139‑1 (MQ=255)                                                                                                    tGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        <  2:501862/65‑1 (MQ=255)                                                                                                    tGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        >  1:501862/1‑65 (MQ=255)                                                                                                    tGCGGTACTGGGCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTg                              >  2:10552/1‑139 (MQ=255)                                                                                                              ggCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGcc                                                                                                             <  2:253006/50‑1 (MQ=255)                                                                                                              ggCATTTACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGcc                                                                                                             >  1:253006/1‑50 (MQ=255)                                                                                                                   ttACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        <  2:272406/50‑1 (MQ=255)                                                                                                                   ttACCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        >  1:272406/1‑50 (MQ=255)                                                                                                                     aCCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGc                                                                                                          >  2:667913/1‑46 (MQ=255)                                                                                                                     aCCCTACAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGc                                                                                                          <  1:667913/46‑1 (MQ=255)                                                                                                                          aCAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        <  1:838212/43‑1 (MQ=255)                                                                                                                          aCAAACTGCTGCCTCACAGGAGCGTGAAGAGAATCGCCTGCCg                                                                                                        >  2:838212/1‑43 (MQ=255)                                                                                                                               ctgctgCCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGATTGCGCCCAAACCCCTGGATg   <  1:10552/139‑1 (MQ=255)                                                                                                                                tgctgcCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGATTGCGCCCAAACCCCTGGATGc  >  2:480192/1‑139 (MQ=255)                                                                                                                                tgctgcCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGATTGCGCCCAAACCCCTGGATGc  >  2:81320/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                      AAACAATCTCTAGACCATCCTTAACGATTCAGCCACTTTTTTATGTTGCTTTTTTGTAAACAGATTAACACCTCGTCAAAATCCTGCTATTCTGCCCGTTGCGGTACTGGGCATTTACCCTACAAACTGCTGTCTCACAGGAGCGTGAAGAGAATCGCCTGCCGCACTATGACAATGAGAGCGAGGAGAACCGTCGTGCTAGAAGAATACCGTAAGCACGTAGCTGAGCGTGCCGCTGAGGGGATTGCGCCCAAACCCCTGGATGC  >  NC_000913/131420‑131685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑