| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,493,219 | A→G | E250E (GAA→GAG) | trg → | methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,493,219 | 0 | A | G | 97.7% | 152.8 / ‑7.2 | 44 | E250E (GAA→GAG) | trg | methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/20); minor base C (0/1); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTG > NC_000913/1493085‑1493352 | ggCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcgg > 2:703009/1‑139 (MQ=255) gcgcGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc > 1:931885/1‑139 (MQ=255) cgcgTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggcg < 1:210116/139‑1 (MQ=255) cgTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCggg < 1:703009/139‑1 (MQ=255) gTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt < 2:952290/139‑1 (MQ=255) gTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt < 2:895978/139‑1 (MQ=255) gCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGaaa > 1:521530/1‑139 (MQ=255) cTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg < 1:733462/139‑1 (MQ=255) ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt > 2:602315/1‑139 (MQ=255) gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTa > 1:951692/1‑139 (MQ=255) gATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcgt < 2:951692/139‑1 (MQ=255) aTGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc > 2:496897/1‑98 (MQ=255) aTGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc < 1:496897/98‑1 (MQ=255) tGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAAt > 2:201202/1‑107 (MQ=255) tGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAAt < 1:201202/107‑1 (MQ=255) tGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTAGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCa > 1:569120/1‑139 (MQ=255) gCTACGTCTGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATg < 2:986712/139‑1 (MQ=255) cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgc > 2:940068/1‑139 (MQ=255) gATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑Ca > 1:622756/1‑139 (MQ=255) tCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACt < 1:929725/139‑1 (MQ=255) aTTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGa < 2:931885/139‑1 (MQ=255) tCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATg < 1:53022/139‑1 (MQ=255) cTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAg > 2:1045597/1‑139 (MQ=255) cTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAg > 1:446930/1‑139 (MQ=255) tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGACCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAgg < 2:674244/139‑1 (MQ=255) tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTAGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAgg < 2:569120/139‑1 (MQ=255) cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGa < 1:166574/139‑1 (MQ=255) cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca > 2:459838/1‑108 (MQ=255) cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca < 1:459838/108‑1 (MQ=255) cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGa > 2:902577/1‑139 (MQ=255) cacaACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACa > 2:300385/1‑139 (MQ=255) cacaACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACa < 2:521530/139‑1 (MQ=255) cacaACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACa > 1:744877/1‑139 (MQ=255) aaCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACAggg > 2:795303/1‑139 (MQ=255) cGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACAGGgcg > 2:30554/1‑139 (MQ=255) aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACt < 2:876248/104‑1 (MQ=255) aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACt > 1:876248/1‑104 (MQ=255) tCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTa > 2:325763/1‑139 (MQ=255) cGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCAcc < 1:300385/139‑1 (MQ=255) atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt > 2:1038129/1‑55 (MQ=255) atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt < 1:1038129/55‑1 (MQ=255) atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATg > 2:426718/1‑74 (MQ=255) atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATg < 1:426718/74‑1 (MQ=255) tgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTg > 1:567681/1‑139 (MQ=255) | GGCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATT‑‑CACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTG > NC_000913/1493085‑1493352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |