Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,533,045 C→T intergenic (+93/‑7) ydcC → / → pptA H repeat‑associated putative transposase/4‑oxalocrotonate tautomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,533,0450CT98.7% 234.5 / NA 75intergenic (+93/‑7)ydcC/pptAH repeat‑associated putative transposase/4‑oxalocrotonate tautomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (37/37);  minor base A (1/0);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTG  >  NC_000913/1532912‑1533178
                                                                                                                                     |                                                                                                                                     
aCTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAAt                                                                                                                                  >  1:263294/1‑139 (MQ=255)
    ccTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATg                                                                                                                              >  2:938504/1‑139 (MQ=255)
        gCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGc                                                                                                                          >  1:499758/1‑139 (MQ=255)
              gTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCg                                                                                                                    <  1:733123/139‑1 (MQ=255)
                  ttACGTGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACAt                                                                                                                <  2:499758/139‑1 (MQ=255)
                        ggAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATg                                                                                                          >  1:1048545/1‑139 (MQ=255)
                          aGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGtt                                                                                                        >  2:177581/1‑139 (MQ=255)
                           gCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGttt                                                                                                       >  1:940196/1‑139 (MQ=255)
                            cGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGtttt                                                                                                      <  1:425864/139‑1 (MQ=255)
                                cTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCcgc                                                                                                  <  1:633528/139‑1 (MQ=255)
                                 tttCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGTCATTAAATGTTTTCcgcg                                                                                                 <  2:585459/139‑1 (MQ=255)
                                    cGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGa                                                                                              <  1:80438/139‑1 (MQ=255)
                                    cGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGa                                                                                              <  1:679047/139‑1 (MQ=255)
                                             gCCCTGGGCATTATTGTCGTATAAACGCTTTACCAGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGa                                                                                     >  1:1046232/1‑139 (MQ=255)
                                                   ggCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAacaaca                                                                               >  2:474470/1‑139 (MQ=255)
                                                       ttattGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACaaaaa                                                                           <  1:970115/139‑1 (MQ=255)
                                                             tCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAgcagca                                                                     >  2:911022/1‑139 (MQ=255)
                                                               gTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACt                                                                   >  1:791380/1‑139 (MQ=255)
                                                                 ataAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtg                                                                 <  2:471417/139‑1 (MQ=255)
                                                                     aCGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctgc                                                             <  1:515206/139‑1 (MQ=255)
                                                                      cGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCa                                                            <  1:938504/139‑1 (MQ=255)
                                                                          ttACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGata                                                        >  2:46761/1‑139 (MQ=255)
                                                                          ttACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGata                                                        >  1:42496/1‑139 (MQ=255)
                                                                           tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGatat                                                       >  2:516887/1‑139 (MQ=255)
                                                                           tACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGatat                                                       >  2:858326/1‑139 (MQ=255)
                                                                              cGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTAc                                                    <  1:46761/139‑1 (MQ=255)
                                                                              cGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTAc                                                    <  1:858326/139‑1 (MQ=255)
                                                                              cGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTAc                                                    <  2:791380/139‑1 (MQ=255)
                                                                                 acacATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa                                                 <  1:516887/139‑1 (MQ=255)
                                                                                  cacaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctg                                                              >  2:414069/1‑125 (MQ=255)
                                                                                  cacaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgctg                                                              <  1:414069/125‑1 (MQ=255)
                                                                                   acaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACt                                                                   <  2:31542/119‑1 (MQ=255)
                                                                                   acaTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGAGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACt                                                                   >  1:31542/1‑119 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGc                                                                                                                             <  2:539773/59‑1 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGc                                                                                                                             >  1:539773/1‑59 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAagca                                                                        >  1:871745/1‑112 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAagca                                                                        <  2:871745/112‑1 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTActg                                                  >  1:882933/1‑132 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTActg                                                  <  2:882933/134‑3 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa                                                 <  2:157453/135‑1 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa                                                 >  1:157453/1‑135 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGtt                                             >  2:1038418/1‑139 (MQ=255)
                                                                                     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGtt                                             >  1:189930/1‑139 (MQ=255)
                                                                                       gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                           >  1:612037/1‑139 (MQ=255)
                                                                                       gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                           <  2:925623/139‑1 (MQ=255)
                                                                                       gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                           <  2:189930/139‑1 (MQ=255)
                                                                                                  tgtgtgTTAATTTTACTGACATCTATCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCCCTTGCTGCAGATATTACTGACGTTATTATTCGTCATc                                >  2:276428/1‑139 (MQ=255)
                                                                                                    tgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATc                                                                                                                     <  1:883088/52‑1 (MQ=255)
                                                                                                    tgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATc                                                                                                                     >  2:883088/1‑52 (MQ=255)
                                                                                                    tgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTg                              >  1:195734/1‑139 (MQ=255)
                                                                                                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                             <  1:119142/139‑1 (MQ=255)
                                                                                                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                             >  2:947449/1‑139 (MQ=255)
                                                                                                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                             <  2:1046232/139‑1 (MQ=255)
                                                                                                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                             <  1:911022/139‑1 (MQ=255)
                                                                                                      tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATc                                                                                                                     <  2:516251/50‑1 (MQ=255)
                                                                                                      tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATc                                                                                                                     >  1:516251/1‑50 (MQ=255)
                                                                                                      tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGaa                            >  1:475260/1‑139 (MQ=255)
                                                                                                        ttAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAagca                                                                        <  2:468648/93‑1 (MQ=255)
                                                                                                        ttAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAagca                                                                        >  1:468648/1‑93 (MQ=255)
                                                                                                              ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGaa                                                                                    <  2:51358/75‑1 (MQ=255)
                                                                                                              ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGaa                                                                                    >  1:51358/1‑75 (MQ=255)
                                                                                                              ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgct                                                               >  2:260148/1‑96 (MQ=255)
                                                                                                              ttACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTtgct                                                               <  1:260148/96‑1 (MQ=255)
                                                                                                               tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCAt                                 >  2:85842/1‑125 (MQ=255)
                                                                                                               tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCAt                                 <  1:85842/125‑1 (MQ=255)
                                                                                                               tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGa                   >  1:893670/1‑139 (MQ=255)
                                                                                                                aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGAc                  >  2:805579/1‑139 (MQ=255)
                                                                                                                      aTCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACACAAAAGACAGTTCg            >  2:98785/1‑139 (MQ=255)
                                                                                                                         tttCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTActg                                                  >  2:892093/1‑96 (MQ=255)
                                                                                                                         tttCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTActg                                                  <  1:892093/98‑3 (MQ=255)
                                                                                                                           tCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAg       >  1:454640/1‑139 (MQ=255)
                                                                                                                             aaaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCa     <  2:9644/139‑1 (MQ=255)
                                                                                                                              aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAt    <  1:975152/139‑1 (MQ=255)
                                                                                                                              aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGGTCGATAAGTAt    <  2:612037/139‑1 (MQ=255)
                                                                                                                                aGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTg  <  2:263294/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
ACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTGCCCTGGGCATTATTGTCGTATAAACGCTTTACCGGACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTG  >  NC_000913/1532912‑1533178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: