Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,533,129	C→T	T26T (ACC→ACT)	pptA →	4‑oxalocrotonate tautomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,533,129	0	C	T	100.0%	213.0 / NA	65	T26T (ACC→ACT)	pptA	4‑oxalocrotonate tautomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/29);  total (36/29)

ACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAA  >  NC_000913/1532993‑1533261                                                                                                                                         |                                                                                                                                     acacATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa                                                                                                                                    <  1:516887/139‑1 (MQ=255)     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa                                                                                                                                    >  1:157453/1‑135 (MQ=255)     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa                                                                                                                                    <  2:157453/135‑1 (MQ=255)     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGtt                                                                                                                                >  1:189930/1‑139 (MQ=255)     aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGtt                                                                                                                                >  2:1038418/1‑139 (MQ=255)       gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                                                                                                              >  1:612037/1‑139 (MQ=255)       gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                                                                                                              <  2:189930/139‑1 (MQ=255)       gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                                                                                                              <  2:925623/139‑1 (MQ=255)                  tgtgtgTTAATTTTACTGACATCTATCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCCCTTGCTGCAGATATTACTGACGTTATTATTCGTCATc                                                                                                                   >  2:276428/1‑139 (MQ=255)                    tgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTg                                                                                                                 >  1:195734/1‑139 (MQ=255)                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                <  1:911022/139‑1 (MQ=255)                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                <  2:1046232/139‑1 (MQ=255)                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                >  2:947449/1‑139 (MQ=255)                     gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                <  1:119142/139‑1 (MQ=255)                      tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGaa                                                                                                               >  1:475260/1‑139 (MQ=255)                               tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCAt                                                                                                                    >  2:85842/1‑125 (MQ=255)                               tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCAt                                                                                                                    <  1:85842/125‑1 (MQ=255)                               tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGa                                                                                                      >  1:893670/1‑139 (MQ=255)                                aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGAc                                                                                                     >  2:805579/1‑139 (MQ=255)                                      aTCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACACAAAAGACAGTTCg                                                                                               >  2:98785/1‑139 (MQ=255)                                           tCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAg                                                                                          >  1:454640/1‑139 (MQ=255)                                             aaaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCa                                                                                        <  2:9644/139‑1 (MQ=255)                                              aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAt                                                                                       <  1:975152/139‑1 (MQ=255)                                              aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGGTCGATAAGTAt                                                                                       <  2:612037/139‑1 (MQ=255)                                                aGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTg                                                                                     <  2:263294/139‑1 (MQ=255)                                                   agtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGctc                                                                                  >  1:350450/4‑139 (MQ=255)                                                    gtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGAt                                                                                             <  2:396141/125‑1 (MQ=255)                                                    gtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGAt                                                                                             >  1:396141/3‑127 (MQ=255)                                                    gtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGctct                                                                                 >  1:435678/3‑139 (MQ=255)                                                     tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                >  2:986383/2‑107 (MQ=255)                                                     tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                <  1:986383/106‑1 (MQ=255)                                                     tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTTCTCTa                                                                                >  2:865069/2‑139 (MQ=255)                                                     tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTa                                                                                >  2:477997/2‑139 (MQ=255)                                                             tGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc                                                                                                                         <  2:340341/90‑1 (MQ=255)                                                             tGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc                                                                                                                         >  1:340341/1‑90 (MQ=255)                                                              gCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                <  1:823712/98‑1 (MQ=255)                                                              gCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                >  2:823712/1‑98 (MQ=255)                                                              gCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGAtt                                                                       >  1:205677/1‑139 (MQ=255)                                                               ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc                                                                                                                         >  1:133817/1‑88 (MQ=255)                                                               ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc                                                                                                                         <  2:133817/88‑1 (MQ=255)                                                               ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTc                                                                      >  1:219866/1‑139 (MQ=255)                                                               ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTc                                                                      >  1:646504/1‑139 (MQ=255)                                                                 gCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCaa                                                                    >  1:526044/1‑139 (MQ=255)                                                                    caTCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                                                                                                              >  2:1004790/1‑78 (MQ=255)                                                                    caTCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat                                                                                                                              <  1:1004790/78‑1 (MQ=255)                                                                       cGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc                                                                                                                         >  1:320227/1‑80 (MQ=255)                                                                       cGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc                                                                                                                         <  2:320227/80‑1 (MQ=255)                                                                       cGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGaa                                                              <  1:1038418/139‑1 (MQ=255)                                                                               aaTGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCa                                                      <  2:1048545/139‑1 (MQ=255)                                                                                    tttCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTa                                                 >  2:196279/1‑139 (MQ=255)                                                                                          cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAAc                                                                                                              <  2:615929/72‑1 (MQ=255)                                                                                          cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAAc                                                                                                              >  1:615929/1‑72 (MQ=255)                                                                                          cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCaa                                                     <  1:615087/129‑1 (MQ=255)                                                                                          cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCaa                                                     >  2:615087/1‑129 (MQ=255)                                                                                          cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTggg                                           >  2:317913/1‑139 (MQ=255)                                                                                          cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTggg                                           >  2:72720/1‑139 (MQ=255)                                                                                              aaCTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGc                                       >  1:243783/1‑139 (MQ=255)                                                                                                   gACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                >  1:763227/1‑61 (MQ=255)                                                                                                   gACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa                                                                                                                <  2:763227/61‑1 (MQ=255)                                                                                                           acaaAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGccc                          <  2:350450/139‑1 (MQ=255)                                                                                                                           gctgcAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGa          >  2:189757/1‑139 (MQ=255)                                                                                                                               cAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATaaa      <  1:177581/139‑1 (MQ=255)                                                                                                                                 gATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGa    <  1:474470/139‑1 (MQ=255)                                                                                                                                 gATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGa    <  1:72720/139‑1 (MQ=255)                                                                                                                                   tatTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGaaa  <  2:195734/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     ACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAA  >  NC_000913/1532993‑1533261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑