Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,621,542	G→A	D71N (GAC→AAC)	ydeE →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,621,542	0	G	A	100.0%	162.7 / NA	52	D71N (GAC→AAC)	ydeE	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

TGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTA  >  NC_000913/1621411‑1621674                                                                                                                                    |                                                                                                                                     tGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGaa                                                                                                                               <  2:709067/139‑1 (MQ=255)   ccATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAAc                                                                                                                             >  1:744063/1‑139 (MQ=255)       ttATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCta                                                                                                                         <  1:365066/139‑1 (MQ=255)        tATGACCATTTACTTGAGTCG‑CAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtata                                                                                                                       <  2:1051774/139‑1 (MQ=255)                tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACt                                                                                                                >  1:142192/1‑139 (MQ=255)                tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACt                                                                                                                >  1:92857/1‑139 (MQ=255)                          tCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTAcc                                                                                                      >  1:10778/1‑139 (MQ=255)                                          aGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtt                                                                                      >  2:730569/1‑139 (MQ=255)                                           gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGttt                                                                                     >  1:471565/1‑139 (MQ=255)                                            tgtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtttt                                                                                    <  2:119284/139‑1 (MQ=255)                                              tCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTAt                                                                                  >  1:247264/1‑139 (MQ=255)                                                gATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                                <  2:830095/91‑1 (MQ=255)                                                gATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                                >  1:830095/1‑91 (MQ=255)                                                gATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTg                                                                                >  1:182714/1‑139 (MQ=255)                                                  tCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGcc                                                                              <  2:740888/139‑1 (MQ=255)                                                      aTCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          <  1:730569/139‑1 (MQ=255)                                                      aTCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          >  2:666719/1‑139 (MQ=255)                                                        cGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                                        <  1:639715/139‑1 (MQ=255)                                                          gTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACttt                                                                      <  2:215055/139‑1 (MQ=255)                                                            tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTgg                                                                                                              >  2:1034601/1‑97 (MQ=255)                                                            tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTgg                                                                                                              <  1:1034601/97‑1 (MQ=255)                                                            tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTa                                                                     >  2:980402/1‑138 (MQ=255)                                                            tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTa                                                                     <  1:980402/138‑1 (MQ=255)                                                            tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAg                                                                    >  1:813491/1‑139 (MQ=255)                                                              tGCGATGACAATTGCGCTCACTATTGGCGTCGGTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTg                                                                  >  2:774922/1‑139 (MQ=255)                                                                          tGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACg                                                      >  2:173941/1‑139 (MQ=255)                                                                            cgcTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGt                                                          >  1:206771/1‑133 (MQ=255)                                                                            cgcTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGt                                                          <  2:206771/133‑1 (MQ=255)                                                                             gcTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGc                                                                                                             >  2:693305/1‑81 (MQ=255)                                                                             gcTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGc                                                                                                             <  1:693305/81‑1 (MQ=255)                                                                              cTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTgg                                                  >  1:135921/1‑139 (MQ=255)                                                                               tCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGt                                                 >  1:1000378/1‑139 (MQ=255)                                                                                cACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGtt                                                <  1:666719/139‑1 (MQ=255)                                                                                     ttGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGct                                           <  2:751272/139‑1 (MQ=255)                                                                                           tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                                        >  2:338559/1‑104 (MQ=255)                                                                                           tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                                        <  1:338559/104‑1 (MQ=255)                                                                                             gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg                                   >  1:425930/1‑139 (MQ=255)                                                                                             gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg                                   <  2:471565/139‑1 (MQ=255)                                                                                                        cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          >  2:390707/1‑89 (MQ=255)                                                                                                        cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          <  1:390707/89‑1 (MQ=255)                                                                                                                 tATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTAt               <  1:774922/139‑1 (MQ=255)                                                                                                                   tCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTgg                                                  <  1:2666/102‑1 (MQ=255)                                                                                                                   tCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTgg                                                  >  2:2666/1‑102 (MQ=255)                                                                                                                     cTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          >  1:884263/1‑76 (MQ=255)                                                                                                                     cTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          <  2:884263/76‑1 (MQ=255)                                                                                                                       ggCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGcc                                 >  1:256519/1‑115 (MQ=255)                                                                                                                       ggCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGcc                                 <  2:256519/115‑1 (MQ=255)                                                                                                                             tAAGTTCAACAAGAAACGCTATATGTTACTGGCACTTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTgg                                                  >  2:522651/1‑92 (MQ=255)                                                                                                                             tAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTgg                                                  <  1:522651/92‑1 (MQ=255)                                                                                                                             tAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCt   >  2:603871/1‑139 (MQ=255)                                                                                                                             tAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCt   >  2:819024/1‑139 (MQ=255)                                                                                                                              aaGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTa  <  1:173941/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     TGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTA  >  NC_000913/1621411‑1621674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑