Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,673,314 A→G L63P (CTG→CCG)  mdtJ ← multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,673,3140AG100.0% 154.0 / NA 43L63P (CTG→CCG) mdtJmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

ACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCG  >  NC_000913/1673181‑1673446
                                                                                                                                     |                                                                                                                                    
aCGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCa                                                                                                                                 <  1:64772/139‑1 (MQ=255)
  gggTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATa                                                                                                                               >  1:123782/1‑139 (MQ=255)
       ccTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTa                                                                                                                          >  1:1011069/1‑139 (MQ=255)
       ccTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTa                                                                                                                          >  2:37698/1‑139 (MQ=255)
               tATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGgcgc                                                                                                                                  <  1:133949/123‑1 (MQ=255)
               tATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGgcgc                                                                                                                                  >  2:133949/1‑123 (MQ=255)
                     cacaATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGAtt                                                                                                            >  1:404722/1‑139 (MQ=255)
                         aTCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGAtttttt                                                                                                        >  2:507267/1‑139 (MQ=255)
                          tCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGAttttttt                                                                                                       >  1:975793/1‑139 (MQ=255)
                            ccGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTaa                                                                                                     <  1:274110/139‑1 (MQ=255)
                             cgccGACCAGGATGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCAAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAAc                                                                                                    >  2:612107/4‑139 (MQ=255)
                                cGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGc                                                                                                 >  2:1047673/1‑139 (MQ=255)
                                   ccAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGaa                                                                                              <  1:1047673/139‑1 (MQ=255)
                                      gggTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAaga                                                                                           <  2:975793/139‑1 (MQ=255)
                                      gggTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAaga                                                                                           <  2:864528/139‑1 (MQ=255)
                                       ggtggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagag                                                                                          <  1:37698/139‑1 (MQ=255)
                                           gtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGaa                                                                                      <  1:507267/139‑1 (MQ=255)
                                                cccGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAatata                                                                                 <  2:123782/139‑1 (MQ=255)
                                                cccGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAatata                                                                                 <  1:986178/139‑1 (MQ=255)
                                                   ggCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATAc                                                                              >  1:408940/1‑139 (MQ=255)
                                                                    aTAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAc                                                             <  1:348563/139‑1 (MQ=255)
                                                                                   ataacataacGCTAAAATAGGTATAAAATAAAGTACCAATCCCTTCCCACGGCCCATAGGCTAATGCTAAGGCGATTTTTTTAAATGCAAATGATAGAAATAGAGACGACAGAGAAATCATCACCTTCATTAAAATaaa                                              <  2:746077/139‑1 (MQ=255)
                                                                                   aTAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAATCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                            <  2:720229/125‑1 (MQ=255)
                                                                                   aTAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                            >  1:720229/1‑125 (MQ=255)
                                                                                            cGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                            <  1:554104/116‑1 (MQ=255)
                                                                                            cGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                            >  2:554104/1‑116 (MQ=255)
                                                                                               tAAACAAGGTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTcc                                  >  2:385714/1‑139 (MQ=255)
                                                                                                       gTAATAAATAAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCCCACTGa                          >  1:541386/1‑139 (MQ=255)
                                                                                                         aataaataAAATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCCCACTGACg                        <  2:408940/139‑1 (MQ=255)
                                                                                                           taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGTCGATTTTTTTAACGGCGAAAGAGAGAAatata                                                                                 <  1:186390/80‑1 (MQ=255)
                                                                                                           taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAgagaga                                                                                       >  1:510020/1‑74 (MQ=255)
                                                                                                           taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAgagaga                                                                                       <  2:510020/74‑1 (MQ=255)
                                                                                                           taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAatata                                                                                 >  2:186390/1‑80 (MQ=255)
                                                                                                           taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTccc                                 <  2:566951/128‑1 (MQ=255)
                                                                                                           taaataaaATACCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTccc                                 >  1:566951/1‑128 (MQ=255)
                                                                                                                 aaataCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGaaa                                                                                             >  2:631945/1‑62 (MQ=255)
                                                                                                                 aaataCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGaaa                                                                                             <  1:631945/62‑1 (MQ=255)
                                                                                                                  aataCCGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCCCACTGACGCTCGCCCAt               >  1:448725/1‑139 (MQ=255)
                                                                                                                       cGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCCCACTGACGCTCGCCCATTTCAt          >  1:882133/1‑139 (MQ=255)
                                                                                                                       cGATACCTTCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCCCACTGACGCTCGCCCATTTCAt          >  1:653378/1‑139 (MQ=255)
                                                                                                                               tCCCACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCCCACTGACGCTCGCCCATTTCATTGACAGCg  <  2:1011069/139‑1 (MQ=255)
                                                                                                                                 ccACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagagag                                                                                        >  2:46371/1‑51 (MQ=255)
                                                                                                                                 ccACGGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAagagag                                                                                        <  1:46371/51‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
ACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCG  >  NC_000913/1673181‑1673446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: