Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,708,533	C→T	Q539* (CAG→TAG)	rsxC →	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,533	0	C	T	100.0%	98.1 / NA	31	Q539* (CAG→TAG)	rsxC	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/16);  total (15/16)

AGAAACAGGCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC  >  NC_000913/1708423‑1708656                                                                                                               |                                                                                                                            agaAACAGGCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACgc                                                                                                 <  2:436188/139‑1 (MQ=255)   aaaCAGGCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACgcag                                                                                               >  2:79195/1‑139 (MQ=255)       aGGCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAAc                                                                                           <  1:747079/139‑1 (MQ=255)            cAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTg                                                                                      <  1:973685/139‑1 (MQ=255)                    acaGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCaaa                                                                                                           <  2:830729/110‑1 (MQ=255)                    acaGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCaaa                                                                                                           >  1:830729/1‑110 (MQ=255)                        ccTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTaa                                                                                                       >  2:83046/1‑110 (MQ=255)                        ccTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTaa                                                                                                       <  1:83046/110‑1 (MQ=255)                        ccTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTa                                                                          >  2:926269/1‑139 (MQ=255)                        ccTACTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCATCAACCGTTGCTGATCCACGTa                                                                          >  2:614856/1‑139 (MQ=255)                               tgATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGc                                                                   <  2:177175/139‑1 (MQ=255)                                 aTTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCg                                                                 >  2:147783/1‑139 (MQ=255)                                   tAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGtt                                                               <  1:147783/139‑1 (MQ=255)                                   tAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGtt                                                               <  1:301636/139‑1 (MQ=255)                                      aGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGaa                                                            >  1:140018/1‑139 (MQ=255)                                       gCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAag                                                           <  1:783036/139‑1 (MQ=255)                                          ggCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAagcag                                                        >  2:1038191/1‑139 (MQ=255)                                                           cAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCaaa                                       <  1:197378/139‑1 (MQ=255)                                                                cAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAgcgcg                                  >  2:781339/1‑139 (MQ=255)                                                                cAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCACGCCAAAgcgcg                                  >  2:275218/1‑139 (MQ=255)                                                                   ttattGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCaa                               <  2:492734/139‑1 (MQ=255)                                                                           gcaCGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAAc                       >  2:500602/1‑139 (MQ=255)                                                                           gcaCGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAAc                       <  1:79195/139‑1 (MQ=255)                                                                            caCGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAag                                                           <  2:623236/102‑1 (MQ=255)                                                                            caCGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAag                                                           >  1:623236/1‑102 (MQ=255)                                                                                ggAAGCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCaa                  >  2:359597/1‑139 (MQ=255)                                                                                    gCCCGTAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAgg              >  1:614456/1‑139 (MQ=255)                                                                                         tAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGTCCGCGCCAAAgcgcg                                  <  2:164227/114‑1 (MQ=255)                                                                                         tAAAGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGTCCGCGCCAAAgcgcg                                  >  1:164227/1‑114 (MQ=255)                                                                                           aaGCGCAAGCCAGAGCGAAATAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGc       <  1:80532/139‑1 (MQ=255)                                                                                                cAAGCCAGAGCGAAATAGGCAGAACTGCAGCGAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc  <  2:656635/139‑1 (MQ=255)                                                                                                               |                                                                                                                            AGAAACAGGCCCAGGCTACACAGCCTATTGTGATTAAAGCGGGCGAACGCCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC  >  NC_000913/1708423‑1708656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑